Biosensor comprising linker material and quantum dot beads, and target antigen detection method using same

A detection method, quantum dot technology, applied in the field of biosensors, can solve problems such as limitations, and achieve the effect of amplifying the detection intensity, fast and easy detection and identification, and favorable price competitiveness

Pending Publication Date: 2020-11-24
ZEUS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, in order to be applied to a real lateral flow sensor, a cleaning step is required before adding new g...

Method used

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  • Biosensor comprising linker material and quantum dot beads, and target antigen detection method using same
  • Biosensor comprising linker material and quantum dot beads, and target antigen detection method using same
  • Biosensor comprising linker material and quantum dot beads, and target antigen detection method using same

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0080] [Preparation Example 1] Preparation of Quantum Dots Having Antibodies on Their Surfaces

[0081] (1) Preparation of fat-soluble quantum dots

[0082] In a three-necked flask, 1.0 g of zinc acetate (Zn(Ac) 2 ), 0.441 g of cadmium oxide (CdO), 20 mL of oleic acid, and 75 mL of octadecene (ODE) were mixed and at 150° C. for 1 hour under a nitrogen atmosphere to remove water. Subsequently, the resulting flask was heated to 300° C., and then 1 mL of trioctyl (TOP) and 0.045 g of selenium (Se) were injected and heated for 3 minutes, whereby quantum dot cores were formed.

[0083] Subsequently, 0.5 mL of dodecanethiol was added into the three-necked flask and reacted for 10 minutes. Then, a solution containing 1 mL of TOP and 0.025 g of sulfur (S) was added to the reaction vessel of the three-necked flask and reacted for 20 minutes, whereby a shell was formed. Then, the resulting core and shell were purified with a mixed solution of ethanol and toluene and dissolved in an o...

preparation Embodiment 2

[0093] [Preparation Example 2] Preparation of Quantum Dot Beads Having Antibodies on Their Surfaces

[0094] (1) Synthesis and surface modification of silica particle substrate

[0095] Silica-based nanoparticles were synthesized by the Stober method. First, the NH 4 OH, EtOH, and H 2 O was stirred in an Erlenmeyer flask at a ratio of 3:60:1 mL, 2 mL of tetraethylorthosilicate (TEOS) was added to the reactant, and the mixture was stirred and reacted at 50° C. for 18 hours or more. Here, the reaction time and mixing ratio can be adjusted according to the desired size. Subsequently, final samples were obtained by centrifugation using ethanol. Herein, silica beads of about 200 nm can be obtained.

[0096] Subsequently, for the reaction between the surface and the quantum dots, as reactive functional groups, 180 μL of 3-mercaptopropyltrimethoxysilane (MPTS) and NH 4 OH and react for 12 to 24 hours. After purification by centrifugation using ethanol, a surface-modified silic...

experiment Embodiment 1

[0106] [Experimental Example 1] Confirmation of Characteristics of Quantum Dots and Quantum Dot Beads

[0107] (1) Zeta potential of quantum dots and quantum efficiency of quantum dots and quantum dot beads

[0108] The zeta potential of the quantum dots of Preparation Examples 1-(2) and 1-(3) was measured using ELS-100ZS (Otsuka), and at figure 2 The results are shown in .

[0109] The quantum efficiencies of the quantum dots of Preparation Example 1-(2) and the quantum dot beads of Preparation Example 2-(3) were measured using QE 2000 (Otsuka Corp.), and image 3 The results are shown in . According to these results, according to one aspect of the present disclosure, the quantum dots and quantum dot beads prepared in Preparation Examples 1-(2) and 2-(3) exhibited quantum efficiencies of 92±3% and 83±3%, respectively And since both are greater than 80%, it shows an excellent effect.

[0110] (3) Confirmation of the size and shape of quantum dots and quantum dot beads

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Abstract

One aspect of the present disclosure relates to an immunochromatographic detection method for a target antigen in a biological sample, comprising the step of joining a linker having a first antibody and a quantum dot bead having a second antibody, with respect to the target antigen. By using the quantum dot beads and the linker, the method can successfully amplify detection strength and significantly increase detection sensitivity through a simple process without causing a loss of antigens participating in detection when using only quantum dot beads. Furthermore, the present disclosure can significantly amplify detection strength without an additional washing step, thus enabling excellent detection and identification of a physiological substance in a biological sample, even in an actual product, and can be used to provide a product with a competitive price.

Description

technical field [0001] The present disclosure relates to a biosensor including a linker fluorescent substance and a quantum dot bead and a target antigen detection method using the same. Background technique [0002] In recent years, diseases have become diverse and the relationship between physiological substances present in biological samples such as blood or urine and the disease or physical condition of a subject is being extensively studied and revealed. During this process, technologies that can quickly, accurately and easily detect and identify disease-related physiological substances in biological samples are required. [0003] As representative techniques for detecting physiological substances, there are immunoassay techniques using biomarkers for physiological substances, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), Western blotting, and the like. However, these techniques are complex, time-consuming and expensive, and require a lot o...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/558B01L3/00
CPCG01N33/558B01L3/00G01N33/551G01N33/588G01N33/54306G01N33/54346B01L3/5023G01N2800/00B01L2300/0825G01N33/54386G01N33/533G01N33/553G01N33/54389
Inventor 丁兴琇申圣荣金贤洙朴相绚李智英
Owner ZEUS
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