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Method for treatment of cellular senescence

An anti-aging and extraction technology, applied in pharmaceutical formulas, medical preparations containing active ingredients, anti-toxic agents, etc.

Active Publication Date: 2020-11-24
FARMAR LICENSING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no evidence or public information revealing the anti-aging, whitening or moisturizing effects of S.

Method used

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  • Method for treatment of cellular senescence
  • Method for treatment of cellular senescence
  • Method for treatment of cellular senescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the chromatographic analysis of Herba Sanxae alcohol extract

[0046] Chemical Analysis of the Alcoholic Extract of Herba Sinensis by High Performance Liquid Chromatography Analysis, Operated on a Hitachi 5160 The end-capping tube (5μm) (150 x 4.6mm) of star RP-18 was carried out, and its mobile phase was made of solvent A (0.2wt% phosphoric acid, H 3 PO 4 ) with solvent B (methanol, MeOH), using the following gradient sequence: 0-5 minutes, 30% solvent B; 5-20 minutes, 30-60wt% solvent B; 20-30 minutes, 60-80wt% Solvent B; 30-35 minutes, 80% solvent B was injected at a flow rate of 1.0ml / min, and the injection volume and UV detector were set to 10μl and 203nm, respectively. The results showed that there is a main component in the alcohol extract of Sanxuecao, called 8-O-acetylharpagide (8-O-acetylharpagide) ( figure 1 ).

Embodiment 2

[0047] Embodiment 2, establish human dermal fibroblast (HDF) cell model

[0048] A human dermal fibroblast cell model was established to evaluate aging phenomena using the population doubling formula (PDL) ( figure 2 A). Aging human dermal fibroblasts become flattened and enlarged ( figure 2 C), and the proliferation rate is slow, and the cytoskeleton also expands with the increase of the number of generations ( figure 2 B), In addition, in addition to the changes in cell growth rate and cell morphology, the cell cycle distribution of young and aged cells was also evaluated. The G1 / G0 phase ratio of young human dermal fibroblasts was 72.8%, while aging The G1 / G0 phase ratio of human dermal fibroblasts was 89.1% ( figure 2 D), The results show that the proportion of cells entering cell arrest is increased in aged cells. In addition, aging-associated galactosidase (SA-β-gal) staining was also carried out to confirm that the human dermal fibroblasts at generation Pn+12 ha...

Embodiment 3

[0049] Embodiment 3, Cytotoxicity of Herba Sanxae Alcohol Extract

[0050] MTT test was used to analyze the cytotoxicity of the alcohol extract of Sanxuecao to human dermal fibroblasts, and the results were as follows: image 3 It shows that the survival rate of human dermal fibroblasts treated with a low concentration of Herba Sinensis alcohol extract is even slightly increased. In addition, the semi-lethal concentration (IC50) of the S. Sinensis Alcohol Extract is about 1500 μg / ml , so in the follow-up experiments of the present invention, the concentrations were selected as 50 μg / ml and 100 μg / ml.

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PUM

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Abstract

The present invention provides an alcohol extract of Ajuga taiwanensis Nakai ex Murata which exhibits significant effects on inhibiting the senescence in human WI-38 lung fibroblasts and human dermalfibroblasts. Moreover, the alcohol extract of A. taiwanensis is able to suppress the expression of the cofilin-1, a protein involved in actin dynamics and cell morphology and found to be increased insenescent cells. Suppression effect of cell senescence by this herb extract is more efficient in mild concentration without over-inhibition of cell viability and growth.

Description

technical field [0001] The present invention relates to an extract of Ajuga taiwanensis Nakai ex Murata and a method for treating cell aging. More particularly, the method comprises administering to the subject an effective dose of an anti-aging composition, wherein the anti-aging composition comprises an alcoholic extract of S. taiwanensis. Background technique [0002] Cell senescence is an irreversible physiological process, because the telomeres of chromosomes will gradually lose with the continuous division of cells. In addition, the shape of cells will also become larger and flattened, and enter the G0 phase of the cell cycle to Avoid DNA replication. Although it is impossible to directly control the loss of telomeres in aging cells, many works have highlighted the possibility of temporarily reversing the aging phenotype of aging cells by helping them replenish growth factors, or using growth factors to reshape the cell shape of aging cells. [0003] The onset of agi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P39/06
CPCA61K36/53A61P39/06A61K2236/333A61K2236/55A61P43/00A61K31/7048
Inventor 李易展林云莲黄重生蔡政翰张淳湲林秉泽杜元亨许伟祥罗品荷
Owner FARMAR LICENSING
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