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A way to deal with cellular aging

An anti-aging and composition technology, which is used in pharmaceutical formulations, medical preparations containing active ingredients, antidote, etc.

Active Publication Date: 2022-08-09
FARMAR LICENSING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no evidence or public information revealing the anti-aging, whitening or moisturizing effects of S.

Method used

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  • A way to deal with cellular aging
  • A way to deal with cellular aging
  • A way to deal with cellular aging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1. Chromatographic analysis of the alcohol extract of Radix Radix et Rhizoma

[0046] Chemical analysis of ethanolic extracts of Heliotrope by high performance liquid chromatography, operating on a Hitachi 5160 The end-capping of star RP-18 was performed by tube (5 μm) (150 x 4.6 mm), and its mobile phase consisted of solvent A (0.2 wt% phosphoric acid, H 3 PO 4 ) with solvent B (methanol, MeOH) using the following gradient sequence: 0-5 min, 30% solvent B; 5-20 min, 30-60 wt% solvent B; 20-30 min, 60-80 wt% 30-35 minutes, 80% solvent B, injected at a flow rate of 1.0 ml / min, and the injection volume and UV detector were set to 10 μl and 203 nm, respectively. The results showed that there is a main component in the alcohol extract of Sanxuecao, called 8-O-acetylharpagide (8-O-acetylharpagide) ( figure 1 ).

Embodiment 2

[0047] Example 2. Establishment of human dermal fibroblast (HDF) cell model

[0048] A human dermal fibroblast cell model was established, and the aging phenomenon was assessed using the cell population doubling formula (PDL) ( figure 2 A). Aging human dermal fibroblasts become flattened and enlarged ( figure 2 C), and the proliferation rate is slow, and the cytoskeleton also expands with the increase of the number of passages ( figure 2 B), In addition, in addition to changes in cell growth rate and cell morphology, cell cycle distribution was also assessed for young and aged cells. Young human dermal fibroblasts had a G1 / G0 phase ratio of 72.8%, while aged The G1 / G0 stage ratio of human dermal fibroblasts was 89.1% ( figure 2 D), the results showed that the proportion of cells entering the cell arrest phase increased in aged cells. In addition, aging-associated galactosidase (SA-β-gal) staining was also performed to confirm that human dermal fibroblasts of passage Pn...

Embodiment 3

[0049] Example 3. Cytotoxicity of the alcohol extract of Radix Radix et Rhizoma

[0050] The MTT assay was used to analyze the cytotoxicity of the alcohol extract of Radix Radix et Rhizoma on human dermal fibroblasts, and the results were as follows: image 3 It was shown that the cell viability of human dermal fibroblasts treated with low-concentration ethanolic extract of Radix Radix et Rhizoma was even slightly improved, in addition, the lethal concentration (IC50) of ethanolic extract of Radix Radix et Rhizoma was about 1500μg / ml , so in the subsequent experiments of the present invention, the concentrations were selected as 50 μg / ml and 100 μg / ml.

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PUM

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Abstract

The present invention provides an alcohol extract of Ajuga taiwanensis Nakai ex Murata, which can significantly inhibit the aging phenomenon of human dermal fibroblasts. In addition, the alcohol extract of Ajuga taiwanensis can inhibit cocoa-1 ( Cofilin-1), a protein involved in actin dynamics and cell morphology. At an appropriate concentration, the alcohol extract of Sanxuecao of the present invention can more effectively inhibit the aging of cells without excessively inhibiting the viability and growth of cells.

Description

technical field [0001] The present invention relates to an extract of Ajuga taiwanensis Nakai ex Murata and a method for treating cell senescence. More particularly, the method comprises administering to the subject an effective dose of an anti-aging composition, wherein the anti-aging composition comprises an alcoholic extract of Taiwan Sanxuecao. Background technique [0002] Cellular senescence is an irreversible physiological process, because telomeres are gradually lost as cells divide, and in addition, cells become larger and flatter in shape and enter the G0 phase of the cell cycle. Avoid DNA replication. Although telomere loss in aging cells cannot be directly controlled, many efforts have highlighted the ability to temporarily reverse the aging phenotype by helping aging cells replenish growth factors, or using growth factors to reshape the cell shape of aging cells. [0003] The onset of aging can be easily detected by looking at changes in skin structure, as thi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/56A61K36/53A61P39/06
CPCA61K36/53A61P39/06A61K2236/333A61K2236/55A61P43/00A61K31/7048
Inventor 李易展林云莲黄重生蔡政翰张淳湲林秉泽杜元亨许伟祥罗品荷
Owner FARMAR LICENSING
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