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Preparation method of edible fungus liquid strain

A technology of liquid strains and edible fungi, which is applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of cumbersome and complicated production processes, long production cycles, and high equipment requirements, and achieve shortened cultivation cycles and stable performance , the effect of reducing the pollution rate

Inactive Publication Date: 2020-11-20
平泉市希才应用菌科技发展有限公司
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  • Claims
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Problems solved by technology

[0004] Aiming at the problems that the production process of the existing solid edible fungus strains is cumbersome and complicated, the production cycle is long and the cost is high, and the production of liquid edible fungus strains requires high equipment requirements and the storage time is short, the invention provides a liquid edible fungus The preparation method of strain

Method used

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  • Preparation method of edible fungus liquid strain
  • Preparation method of edible fungus liquid strain
  • Preparation method of edible fungus liquid strain

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Preparation method of mushroom liquid strain (cultivated species)

[0038] 1. Materials and tools

[0039] Millet grains, glycerin, glucose, yeast powder, soybean meal powder, corn flour;

[0040] Bottle (750ml plastic original seed bottle), inoculation tools, ultra-clean bench, beater, liquid fermenter (800L liquid fermenter), liquid inoculation gun.

[0041] 2. Method

[0042] Use millet grains with full grains, no impurities, and a particle size (diameter) of 0.1-0.15 cm as a medium to produce the original seed of shiitake mushrooms. The production process is: soaking the millet grains, pouring water, and bottling (750ml plastic original seed bottle). After bottling, autoclave (keep at 121°C for more than 240 minutes) to ensure thorough sterilization. After the sterilization is completed, inoculate under sterile conditions when the temperature of the medium drops to about 25°C. The inoculation amount is: each 20×200mm inclined test tube with the mother species (th...

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Abstract

The invention relates to the technical field of edible fungus cultivation, and particularly discloses a preparation method of an edible fungus liquid strain. The preparation method of the edible fungus liquid strain comprises the following steps: a, taking grains as a culture medium to culture an edible fungus stock, and after hyphae overgrow, continuing to culture until the grains are softened soas to obtain a grain strain; b, performing low-temperature treatment on the grain strains at 0-4 DEG C, performing crushing under an aseptic condition, adding a glycerol solution, and performing stirring to form suspension slurry; and c, adding the suspension slurry into a culture solution, and carrying out liquid fermentation to obtain the liquid strain. The preparation method of the liquid strain has the advantages of simple process, short production period, low cost, low equipment requirement, long preservation time and the like, and can be widely popularized and used.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for preparing edible fungus liquid strains. Background technique [0002] Most of the edible fungus strains in my country are solid strains, such as shiitake mushroom strains. The shiitake mushroom strains are three-level system, which are mother species, original species and cultivated species, that is, the mother species is transferred to the original species, and the original species is transferred to Cultivated species, cultivated species are transferred to fruiting bags. In the production process of traditional solid edible fungus strains, the process is cumbersome and complicated, the production cycle is long, and the cost is high, and the production and cultivation process of edible fungi is almost controlled by experience. closely related. Compared with solid strains, liquid strains have significant advantages such as short production cycle, lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/40A01G18/20
Inventor 柳凤玉孟凡飞梁晓生张永峰李新
Owner 平泉市希才应用菌科技发展有限公司
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