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High performance liquid chromatography analysis method of hexaflumuron

A high-performance liquid chromatography and analysis method technology, which is applied in analysis materials, material separation, measurement devices, etc., can solve problems such as inability to cover, and achieve the effects of simple detection, guaranteed accuracy, high measurement sensitivity and precision

Pending Publication Date: 2020-11-17
江苏省农产品质量检验测试中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Hexaflumuron is a specific insecticide with high insecticidal activity and broad insecticidal spectrum. It can be used to control various Lepidoptera pests in vegetables, cotton, fruit trees, forest trees and other crops. Pests resistant to esters and pyrethroids have good results; high performance liquid chromatography is one of the most commonly used quantitative analysis methods in the field of pesticide analysis. This method is simple, fast, wide linear range, high accuracy and sensitivity, Has been widely used in the production, quality control and testing standards of hundreds of pesticides; at present, there is no national standard or industry standard for hexaflumuron pesticide products in China, and the relevant literature only involves the high performance liquid chromatography analysis of hexaflumuron in one preparation Method, in the existing analytical method, methanol is selected as the solvent, and 240nm is selected as the detection wavelength. The selections such as solvent and wavelength are only applicable to the detection of emulsifiable concentrate formulations, but cannot cover the detection of baits, bait tablets, suspending agents, microemulsions, water-dispersible granules, Testing requirements for various content dosage forms such as wettable powder and original drug

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  • High performance liquid chromatography analysis method of hexaflumuron
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  • High performance liquid chromatography analysis method of hexaflumuron

Examples

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Embodiment 1

[0032] The present embodiment is a high performance liquid chromatography analysis method of hexaflumuron, comprising the following steps:

[0033] (1) Preparation of standard sample solution: use the mixed solution of dimethylformamide+methanol to dissolve and dilute the standard sample of hexaflumuron to obtain the standard sample solution for later use; specifically: weigh 0.02 g of the standard sample of hexaflumuron, Accurate to 0.0001g, place in a 50mL volumetric flask, dilute to the mark with a mixed solution of dimethylformamide + methanol, cool with ultrasonic vibration, pipette 5mL of the above solution into a 50mL volumetric flask, and then dilute with methanol To the scale, after shaking well, the standard sample solution is prepared; wherein, the volume ratio of dimethylformamide and methanol in the mixed solution of dimethylformamide+methanol is 1:9;

[0034] (2) Preparation of sample solution: use the mixed solution of dimethylformamide+methanol to dissolve the ...

Embodiment 2

[0049] The present embodiment is a high performance liquid chromatography analysis method of hexaflumuron, comprising the following steps:

[0050] (1) Preparation of standard sample solution: use the mixed solution of dimethylformamide+methanol to dissolve and dilute the standard sample of hexaflumuron to obtain the standard sample solution for later use; specifically: weigh 0.02 g of the standard sample of hexaflumuron, Accurate to 0.0001g, place in a 50mL volumetric flask, dilute to the mark with a mixed solution of dimethylformamide + methanol, cool with ultrasonic vibration, pipette 5mL of the above solution into a 50mL volumetric flask, and then dilute with methanol To the scale, after shaking well, the standard sample solution is prepared; wherein, the volume ratio of dimethylformamide and methanol in the mixed solution of dimethylformamide+methanol is 1:9;

[0051] (2) Prepare the sample solution: use the mixed solution of dimethylformamide+methanol to dissolve the hex...

Embodiment 3

[0066] This example is based on Example 1. In this example, weigh 0.02g of hexaflumuron standard sample, accurate to 0.0001g, place it in a 50mL volumetric flask, and use the mixed solution of dimethylformamide + methanol Dilute to the mark, ultrasonically oscillate and cool, pipette 5 mL of the above solution into a 50 mL volumetric flask, dilute with methanol to the mark, and shake well to prepare the standard solution; accurately pipette the above standard solution 1.0 mL, 3.0 mL, 5.0mL, 7.0mL, 10.0mL to 50mL volumetric flasks, dilute to volume with methanol and shake well; The mass concentration of chilumuron is the abscissa, and the peak area of ​​flulumuron is the ordinate, and its linear regression equation is measured, and the linear regression equation is measured as: y=59.702x+88.554, and the correlation coefficient R 2 = 0.9996, proving that hexaflumuron has good linearity in the concentration range of 8.60-85.97mg / L, which meets the requirements of the NY / T 2887-20...

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Abstract

The invention discloses a high performance liquid chromatography analysis method of hexaflumuron. The method comprises the following steps: (1) preparing a standard sample solution; (2) preparing a sample solution; (3) carrying out quantitative analysis on the hexaflumuron sample by using high performance liquid chromatography, wherein a high performance liquid chromatograph with a variable-wavelength ultraviolet detector is adopted; and (4) after the baseline of the high performance liquid chromatograph is stable, continuously injecting a plurality of standard sample solutions, calculating the peak area of hexaflumuron of each needle, and when the relative change of the peak area ratio of hexaflumuron of two adjacent needles is less than 1.5%, performing determining according to the sequence of the standard sample solution, the sample solution, the sample solution and the standard sample solution, and calculating the mass percent content of hexaflumuron in the hexaflumuron sample by using an external standard method; the method is simpler, more convenient, quicker, safer and more accurate in detection of the hexaflumuron pesticide, has higher measurement sensitivity and precision,ensures the detection accuracy, realizes accurate detection of the hexaflumuron pesticide, and has important significance in production and quality detection of pesticide products.

Description

technical field [0001] The invention relates to a method for determining the content of hexaflumuron in pesticides by using high-performance liquid chromatography, in particular to a high-performance liquid chromatography analysis method for hexaflumuron, which belongs to the technical field of pesticide analysis. Background technique [0002] Hexaflumuron is a specific insecticide with high insecticidal activity and broad insecticidal spectrum. It can be used to control various Lepidoptera pests in vegetables, cotton, fruit trees, forest trees and other crops. Pests resistant to esters and pyrethroids have good results; high performance liquid chromatography is one of the most commonly used quantitative analysis methods in the field of pesticide analysis. This method is simple, fast, wide linear range, high accuracy and sensitivity, Has been widely used in the production, quality control and testing standards of hundreds of pesticides; at present, there is no national stand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/74
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/74G01N2030/324
Inventor 徐成辰
Owner 江苏省农产品质量检验测试中心
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