Animal experiment hyperbaric chamber for argon-oxygen gas and animal experiment method thereof
An animal experiment, hyperbaric chamber technology, applied in medical science, veterinary instruments, etc., can solve the problems of high use cost, low degree of automation, expensive argon gas, etc., to avoid carbon dioxide poisoning, improve work efficiency, and reduce material costs. Effect
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[0029] A total of 48 male C56BL / 6 mice were selected. After isoflurane anesthesia, the common carotid artery, internal carotid artery and external carotid artery were bluntly separated. The external carotid artery was cut off, inserted into the internal carotid artery to the middle artery with silicone-coated No. 6 nylon thread, and ligated and fixed. After 1 hour, the nylon thread was pulled out to achieve blood reperfusion, and a mouse cerebral ischemia-reperfusion model was established to simulate the cerebral ischemia and reperfusion injury caused by thrombectomy or thrombolysis in patients with cerebral vascular embolism in clinical practice.
[0030] The mice were randomly divided into model group (MCAO), 50% argon (50% Ar) treatment group, 60% argon treatment (60% Ar) and 75% argon treatment group (50% Ar), each group 12, put the treatment group mice in the animal experiment hyperbaric chamber according to the present invention, close the hatch, set the temperature at 2...
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