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Animal experiment hyperbaric chamber for argon-oxygen gas and animal experiment method thereof

An animal experiment, hyperbaric chamber technology, applied in medical science, veterinary instruments, etc., can solve the problems of high use cost, low degree of automation, expensive argon gas, etc., to avoid carbon dioxide poisoning, improve work efficiency, and reduce material costs. Effect

Inactive Publication Date: 2020-11-13
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method mainly has the following disadvantages: 1. The oxygen concentration in the cabin cannot be dynamically detected; 2. When the oxygen in the cabin is consumed, it cannot be replenished in time and accurately, which may easily cause hypoxia or even death of experimental animals; 3. The carbon dioxide in the cabin cannot be absorbed in time, which will easily lead to carbon dioxide poisoning of animals; 4. The temperature in the cabin cannot be maintained stably; 5. The argon in the cabin cannot be effectively recovered. Due to the high cost of argon, this device has a high cost of use; 6. Low Efficiency, due to the lack of automated control devices, special personnel are required to be on duty when exposed to argon-oxygen mixture for a long time; 7. Most of the existing animal cabins are hypoxic or atmospheric pressure cabins, and the degree of automation is low

Method used

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  • Animal experiment hyperbaric chamber for argon-oxygen gas and animal experiment method thereof
  • Animal experiment hyperbaric chamber for argon-oxygen gas and animal experiment method thereof
  • Animal experiment hyperbaric chamber for argon-oxygen gas and animal experiment method thereof

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Embodiment

[0029] A total of 48 male C56BL / 6 mice were selected. After isoflurane anesthesia, the common carotid artery, internal carotid artery and external carotid artery were bluntly separated. The external carotid artery was cut off, inserted into the internal carotid artery to the middle artery with silicone-coated No. 6 nylon thread, and ligated and fixed. After 1 hour, the nylon thread was pulled out to achieve blood reperfusion, and a mouse cerebral ischemia-reperfusion model was established to simulate the cerebral ischemia and reperfusion injury caused by thrombectomy or thrombolysis in patients with cerebral vascular embolism in clinical practice.

[0030] The mice were randomly divided into model group (MCAO), 50% argon (50% Ar) treatment group, 60% argon treatment (60% Ar) and 75% argon treatment group (50% Ar), each group 12, put the treatment group mice in the animal experiment hyperbaric chamber according to the present invention, close the hatch, set the temperature at 2...

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Abstract

The invention provides an animal experiment hyperbaric chamber for argon-oxygen gas and an animal experiment method of the animal experiment hyperbaric chamber. The animal experiment hyperbaric chamber comprises a chamber body arranged on a base, wherein a chamber door is arranged on a shell of the chamber body, and an oxygen concentration detector, a carbon dioxide concentration detector, a pressure gauge and a temperature probe which are electrically connected with a control system are arranged in the chamber body; an argon inlet pipe one end of which is connected with the cabin body, and the other end of which is connected with an argon bottle; an oxygen inlet pipe one end of which is connected with the chamber body, the other end of which is connected with the oxygen bottle, wherein anelectromagnetic valve is arranged on the oxygen inlet pipe; and an exhaust pipe one end of which is connected with the chamber body, and the other end of which is arranged outside the chamber body. According to the animal experiment hyperbaric cabin for the argon-oxygen gas and the animal experiment method of the animal experiment hyperbaric cabin, automatic regulation and control over the mixingproportion of oxygen, carbon dioxide and argon in the cabin are achieved, meanwhile, the temperature, brightness and pressure in the chamber can be adjusted, visualization of the living state of animals in the cabin is achieved, guarding by specially-assigned persons is not needed, and the working efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to an argon-oxygen gas animal experiment hyperbaric chamber and an animal experiment method thereof. Background technique [0002] Argon is an inert gas that exists in nature. It was previously considered a physiologically inert gas that would not affect the physiological functions of the human body. However, recent studies have found that argon can alleviate ischemic brain injury, reduce the body's inflammatory response, increase the polarization of microglia / macrophages, etc., and its protective effect is concentration (gas partial pressure) dependent. . Based on the advantages of high blood-brain barrier permeability, low side effects and convenient administration of argon gas, many researchers have begun to explore the potential protective effect and mechanism of argon gas under different pressures on various diseases, especially neurological diseases. , to provide a t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 彭彬李霞陈苗苗姜正林
Owner NANTONG UNIVERSITY
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