System, master control device, microcontroller and method for transferring substances into cells

Abstract

The invention provides a system, a master control device, a microcontroller and a method for transmitting substances into cells, and the system comprises a micro-fluidic chip used for fixing cells tobe detected; the objective table is used for placing a micro-fluidic chip fixed with cells to be detected; the objective lens of the microscope is used for optically sampling the to-be-detected cell under the control of the master control device to obtain a sampling picture; the master control device is used for carrying out three-dimensional reconstruction processing on the sampling picture to obtain a three-dimensional reconstruction body corresponding to the to-be-detected cell, and performing geometric analysis on the three-dimensional reconstruction body to obtain an optimal transmissionposition; the microcontroller is used for controlling the microneedle and / or the objective table to move according to the position adjustment instruction, so that the microneedle reaches the optimal transmission position in the to-be-detected cell; and the micro-injector is used for providing ejection pressure for the micro-needle under the control of the master control device and transmitting theexternal substances to be transmitted in the micro-needle into the cells. According to the technical scheme, substances can be efficiently and accurately conveyed into cells, and the conveying success rate is increased.

Description

technical field [0001] The invention relates to the technical field of intracellular transport processing, in particular to a system, a general control device, a microcontroller and a method for transporting substances into cells. Background technique [0002] In microassembly and gene editing, such as transfection of DNA and RNA, it involves the step of transporting foreign substances into the interior of the cell. The current intracellular delivery scheme has the following problems: [0003] Cells need to be fixed before transfer operation to prevent cell movement during operation. Traditional fixation methods, such as the use of microneedles to adsorb cells, cannot fix and arrange more cells in a short period of time, resulting in low intracellular transport efficiency. [0004] Traditional intracellular transport methods are generally biological and chemical transport methods, such as vesicles, viruses, etc. as carriers, and physical methods: electroporation, microflui...

AI Technical Summary

Problems solved by technology

Fatigue and experience will inevitably lead to experimental failure, reducing the efficiency of cell transfer

[0007] In summary, the existing solutions for transporting foreign substances into cells have the following problems: low transmission accuracy, low efficiency, and low success rate

[0008] For the above problems, no effective solutions have been proposed so far

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