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A kind of preparation method and application of liposome containing rose fermented liquid

A fermented liquid and liposome technology, which is applied in the direction of medical preparations containing active ingredients, skin care preparations, pharmaceutical formulas, etc., can solve the problems of easy degradation, poor cell penetration, low bioavailability, and poor stability and other problems, to achieve the effect of promoting penetration and remodeling the skin barrier, the preparation method is simple, and the production cost is low

Active Publication Date: 2022-06-28
上海洁士宝日化集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the polysaccharides and other components in the rose fermentation broth have disadvantages such as poor stability, low bioavailability, easy degradation and poor cell penetration, which limit its efficacy.

Method used

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  • A kind of preparation method and application of liposome containing rose fermented liquid
  • A kind of preparation method and application of liposome containing rose fermented liquid
  • A kind of preparation method and application of liposome containing rose fermented liquid

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0050] (1) Preparation of rose fermentation substrate: The dried rose buds were crushed and passed through a 100-mesh sieve, and water was added to prepare a mixed solution with a mass concentration of 10%, which was sterilized at 121° C. for 15 minutes to obtain a rose fermentation substrate.

[0051] (2) Bacterial activation: Dip Bacillus natto CICC 10262 with a sterilized bamboo stick, streak the solid activated medium according to the plate streak method, and invert aerobic cultivation in a constant temperature incubator at 37°C for 15-20h , to obtain an activated Bacillus natto CICC 10262 strain.

[0052] Dip Lactobacillus bulgaricus CICC 20271 with a sterilized bamboo stick, streak on the solid activated medium according to the plate streaking method, and invert anaerobic cultivation in a constant temperature incubator at 37°C for 15-20 hours to obtain the activated Lactobacillus bulgaricus CICC20271 strain .

[0053] Dip the Saccharomyces cerevisiae CICC 1252 with a st...

Embodiment 1

[0082] Utilize the rose fermentation liquid prepared in Preparation Example 1 to prepare liposomes, and the preparation method of described liposomes comprises the steps:

[0083] (a) Dissolve 100 g of complex phospholipids, 300 g of cholesterol and 30 g of ceramides in a rotary evaporation flask in an appropriate amount of absolute ethanol, and ultrasonically until completely dissolved, wherein the complex phospholipids are a mixture of soybean phospholipids and hydrogenated soybean phospholipids, soybean phospholipids and hydrogenated soybean phospholipids The mass ratio of phospholipids is 10:1;

[0084] (b) the dehydrated alcohol is evaporated with a rotary evaporator, and a uniform and transparent lipid film is formed on the wall of the rotary evaporator, the evaporation temperature is 55 ° C, the evaporation time is 2.5h, and the rotating speed is 50rpm;

[0085](c) 10g rose fermentation broth and 200g pH value are that phosphate buffered solution is added in the describ...

Embodiment 2

[0087] Utilize the rose fermentation broth prepared in Preparation Example 11 to prepare liposomes, and the preparation method of the liposomes comprises the following steps:

[0088] (a) Dissolve 350 g of complex phospholipids, 175 g of cholesterol and 65 g of ceramides in a rotary flask in an appropriate amount of anhydrous ethanol, and sonicate until completely dissolved, wherein the complex phospholipids are a mixture of soybean phospholipids and hydrogenated soybean phospholipids, soybean phospholipids and hydrogenated soybean phospholipids The mass ratio of phospholipids is 7.5:1;

[0089] (b) the dehydrated alcohol is evaporated with a rotary evaporator, a uniform and transparent lipid film is formed on the wall of the rotary evaporator, the evaporation temperature is 60° C., the evaporation time is 2h, and the rotating speed is 75rpm;

[0090] (c) 15g rose fermentation broth and 150g pH value of phosphate buffered solution of 7.4 were added to the rotary flask, mixed u...

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Abstract

The invention relates to a preparation method and application of a liposome containing rose fermented liquid. In the present invention, rose fermented liquid is added, and the rose fermented liquid is prepared by a specific method. The rose fermented liquid of the present invention has relatively high flavonoid and polysaccharide contents, and can regulate skin and scalp flora, and also contains tryptophan , can play the role of soothing and anti-allergic skin; the liposome of the present invention improves its encapsulation stability by adding complex phospholipids, reduces overflow, fully protects the rose fermentation liquid, and adds ceramide and other raw materials to increase skin compatibility , improve the transdermal absorptivity of liposomes, each raw material of the present invention plays the effect of synergism, fully brings into play the moisturizing and anti-aging effect of rose fermented liquid, and through the synergistic effect of rose fermented liquid and each raw material, plays the function of promoting penetration and Remodeling of the skin barrier function; the preparation method of the liposome in the present invention is simple, the production cost is low, and it can be produced on a large scale.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a preparation method and application of a liposome containing rose fermentation liquid. Background technique [0002] Rose not only has ornamental value, but also has certain nutritional and medicinal value, which contains many active ingredients (such as polysaccharides, flavonoids, aromatic oils, etc.) that are beneficial to the human body. The distillation and leaching methods often used in the extraction of rose active substances not only produce limited active substances but also generate a large amount of waste, so using a more environmentally friendly fermentation process can fully extract the active ingredients in roses. CN109806201A discloses that whitening, anti-wrinkle and moisturizing effects can be significantly improved through rose fermentation process. However, the polysaccharides and other components in the rose fermentation broth have disadvantage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/14A61K8/42A61K8/55A61K8/63A61K8/9789A61Q19/00A61Q19/08
CPCA61K8/9789A61K8/553A61K8/63A61K8/42A61K8/14A61Q19/005A61Q19/00A61Q19/08A61K2800/85
Inventor 张启清余海励盛俊娇孙绪友
Owner 上海洁士宝日化集团有限公司
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