Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Myrosinase microcapsule as well as preparation method and application thereof

A technology of myrosinase and microcapsules, which is applied in the directions of microcapsules, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of difficult time guarantee, decreased activity and the like, and achieve the effect of high yield of sulforaphane

Inactive Publication Date: 2020-11-06
NORTHWEST UNIV(CN)
View PDF6 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, although previous studies have reported that it can increase the production of sulforaphane, it is actually the data measured after the glucoraphanin is fully hydrolyzed by myrosinase under the appropriate enzymatic hydrolysis conditions set in the laboratory, and does not represent the actual consumption of sulforaphane by consumers. Such a high yield of sulforaphane can still be obtained, because on the one hand, the activity of myrosinase will decrease under normal temperature storage conditions, and on the other hand, suitable enzymatic hydrolysis conditions such as temperature, time, and pH are difficult to guarantee in the consumption environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Myrosinase microcapsule as well as preparation method and application thereof
  • Myrosinase microcapsule as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of myrosinase microcapsules, comprising the following steps:

[0028] (1) Broccoli seeds, cauliflower seeds, and cabbage seeds with full grains with a germination rate higher than 90% are selected as the source of myrosinase. In this example, the broccoli seeds are selected from Green Doll.

[0029] (2) Keep the selected enzyme source in a 45°C water bath for 10 minutes to inactivate epithelial thiospecific protein (ESP). Because ESP competes with myrosinase, ESP is able to convert glucoraphanin to sulforaphane nitrile, which is biologically inactive.

[0030] (3) The seeds of the inactivated ESP were pulverized, and the Tris-HCl buffer solution of pH 7.5 was added (the Tris-HCl buffer solution contained 0.2M Tris, 0.5mM EDTA, 1.5mM di Thiothreitol and 12.5g / L polyvinylpyrrolidone), after ice-bath extraction for 15min, centrifuge at 10,000 rpm / min for 10min at 4°C, take the supernatant, add ammonium sulfate to the supernatant to make the saturatio...

Embodiment 2

[0034] A preparation method of myrosinase microcapsules, comprising the following steps:

[0035] (1) Broccoli seeds, cauliflower seeds, and cabbage seeds with full grains with a germination rate higher than 90% are selected as the source of myrosinase. In this example, the broccoli seeds are selected from Green Doll.

[0036] (2) Keep the selected enzyme source in a 55°C water bath for 5 minutes to inactivate epithelial thiospecific protein (ESP). Because ESP competes with myrosinase, ESP is able to convert glucoraphanin to sulforaphane nitrile, which is biologically inactive.

[0037] (3) The seeds of inactivated ESP were pulverized, and Tris-HCl buffer solution of pH 7.5 was added with the ratio of solid-liquid mass ratio of 1:12 (the Tris-HCl buffer solution contained 0.2M Tris, 0.5mM EDTA, 1.5mM di Thiothreitol and 12.5g / L polyvinylpyrrolidone), after ice-bath extraction for 15min, centrifuge at 10,000 rpm / min for 10min at 4°C, take the supernatant, add ammonium sulfate...

Embodiment 3

[0041] A preparation method of myrosinase microcapsules, comprising the following steps:

[0042] (1) Broccoli seeds, cauliflower seeds, and cabbage seeds with full grains with a germination rate higher than 90% are selected as the source of myrosinase. In this example, the broccoli seeds are selected from Green Doll.

[0043] (2) Keep the selected enzyme source in a 50°C water bath for 8 minutes to inactivate epithelial thiospecific protein (ESP). Because ESP competes with myrosinase, ESP is able to convert glucoraphanin to sulforaphane nitrile, which is biologically inactive.

[0044] (3) The seeds of inactivated ESP were pulverized, and Tris-HCl buffer solution of pH 7.5 was added with the ratio of solid-liquid mass ratio of 1:10 (the Tris-HCl buffer solution contained 0.2M Tris, 0.5mM EDTA, 1.5mM di Thiothreitol and 12.5g / L polyvinylpyrrolidone), after ice-bath extraction for 15min, centrifuge at 10,000 rpm / min for 10min at 4°C, take the supernatant, add ammonium sulfate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention discloses a myrosinase microcapsule as well as a preparation method and application thereof.The average particle size of the myrosinase microcapsule prepared by the preparation method isless than 10 [mu]m, and the myrosinase microcapsule is suitable for being applied to food without obviously influencing the taste; moreover, the preparation method disclosed by the invention belongsto a normal-temperature preparation method, and the myrosinase microcapsule can be prepared without adopting relatively high temperature in a preparation process, so that the activity of the myrosinase can be maintained, and the influence of high temperature on the activity of the myrosinase is avoided. The activity of the myrosinase microcapsule prepared by the invention is 68% of the activity offree enzyme; the reduction of enzyme activity is 33-52% lower compared with that of free enzyme after the enzyme is placed for 3 months at normal temperature; therefore, the myrosinase microcapsule prepared by the method has high myrosinase activity, the activity of the myrosinase microcapsule can be kept for a long time and cannot be rapidly reduced, and the situation that the quantity of sulforaphane generated by broccoli bud seedlings or sprout powder is low due to rapid reduction of the myrosinase activity is avoided.

Description

technical field [0001] The invention specifically relates to a myrosinase microcapsule and its preparation method and application. Background technique [0002] Sulforaphane is a plant-derived chemical substance with strong anticancer activity discovered so far. Its precursor glucosinolate widely exists in cruciferous plants, and broccoli has the highest content. Studies have pointed out that consuming cruciferous vegetables 3-5 times a week can significantly reduce the risk of lung cancer and bowel cancer by 30-40%. Therefore, sulforaphane has a very good development prospect because of its powerful disease prevention effect. [0003] Broccoli sprouts are a good source of sulforaphane, which is rich in sulforaphane precursor glucoraphanin, which can produce sulforaphane after enzymatic hydrolysis by myrosinase, and then exert physiological effects. Broccoli buds are also rich in polyphenols, flavonoids, vitamins, minerals and other nutrients. Its anti-cancer, anti-cancer,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/47A61K36/31A61K9/50A61K9/46A61K9/00A61P35/00A61P37/04C12N9/24A01C1/08A01C1/02A01G31/00A23L2/39A23L2/52A23L33/10A61K36/736A61K33/30
CPCA61K38/47A61K36/31A61K36/736A61K33/30A61K9/50A61K9/2018A61K9/0095A61K9/0007A61P35/00A61P37/04C12N9/2402C12Y302/01147A01C1/08A01C1/02A01G31/00A23L2/39A23L2/52A23L33/10A23V2002/00A61K2300/00A23V2200/30A23V2250/032A23V2250/642A23V2250/1642
Inventor 吕新刚范代娣孟官丽曹炜汪浪红
Owner NORTHWEST UNIV(CN)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com