Method for simultaneously detecting concentrations of various antipsychotic drugs in serum

Abstract

The invention provides a method for simultaneously detecting the concentrations of various antipsychotic drugs in serum. According to the method, 16 kinds of antipsychotic drugs can be detected at a time; target drugs and metabolites are monitored at the same time; the method is high in sensitivity, high in specificity, good in accuracy and simple in pretreatment process; separation and detectionof the antipsychotic drugs in serum are completed within 5.0 minutes, and the accuracy and precision of the method basically meet requirements; and the method can be used for quantitative analysis ofantipsychotic drugs in serum clinically, and a simple and rapid detection method is provided for concentration monitoring of antipsychotic drugs clinically.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a method for simultaneously detecting the concentration of multiple antipsychotic drugs in serum, wherein the antipsychotic drugs are respectively: N-desmethylclozapine (N-Desmethylclozapine, NCZP), olanzapine (Olanzapine, OZP), Chlorpromazine (Chlorpromazine, CPZ), Clozapine (Clozapine, CZP), Sulpiride (SPR), Amisulpride (Amisulpride, ASP), Haloperidol (Haloperidol, HLP) , Quetiapine (QTP), Norquetiapine (NQTP), Perphenazine (PPZ), Risperidone (RPD), Ziprasidone (ZPD), 9-hydroxy Risperidone (Paliperidone, PPD), Fluphenazine (Fluphenazine, FPN), Dehydroaripiprazole (DHAPZ), Aripiprazole (Aripiprazole, APZ) Background technique [0002] Antipsychotics are drugs indicated for the treatment of a variety of mental disorders, including schizophrenia, hallucinations, mania, sleep disturbances, and dementia. There are 9 categories and more than 40 kinds of antipsyc...

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Problems solved by technology

[0003] At present, the methods for detecting the concentration of antipsychotic drugs in vivo mainly include high-performance liquid chromatography and ultra-high performance liquid chromatography-tandem mass spectrometry. "mass spectrometry with atmospheric pressure chemical ionization" reported a method for detecting 15 psychotropic drugs and some metabolites in plasma, but the sample volume is large, requiring 0.5mL, and SPE is required before, the cost is high, and one needle collection takes 10 minutes, linear Narrow range, etc.; the literature "Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS" reports a method for the detection of multiple psychotropic drugs and metabolites in plasma, which has complex pretreatment, long sampling time, narrow linear range, etc.

Patent CN109668979A discloses a method: "a method for simultaneously detecting 17 antipsychotic drugs in blood samples". This method has a large amount of sample injection, 20 μL per needle; the pretreatment is complicated and requires nitrogen blowing to concentrate and redissolve; it needs to be filtered , the cost is high; the acquisition time is long, it takes 9 minutes

Patent CN110455945A discloses a method and kit for detecting 5 kinds of psychotropic drugs and their main metabolites in blood. This method requires a large sample size of 100 μL, and can only detect 5 kinds of drugs at a time, which cannot meet the high-pass requirements for such drugs. volume screening requirements

In addition, there are patent reports that detect one or several of these drugs, and the efficiency is relatively low

There are deficiencies such as long analysis time for a single sample, low sensitivity, unsuitable linear range, complicated sample pretreatment, and matrix effect interference.

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