Cell block collection device and cell block collection method

Abstract

The invention discloses a cell block collection device and a cell block collection method, and belongs to the technical field of collection of cells in body fluid. The technical problem of low detection rate when cancer detection is carried out by adopting cell slices when the content of cancerous cells in the body fluid is relatively low is solved. The cell collection device comprises a supporting plate, a plurality of grooves are formed in the supporting plate, and an auxiliary material tube, a fixed supporting plate, a special centrifugal tube, a tissue embedding box and embedding paper areclamped in the grooves respectively. A use method of the cell collection device comprises the following steps: preparing raw materials; centrifugally filtering exfoliated cell sap; performing cell precipitation hardening; fixing exfoliated cell blocks; and embedding and paraffinizing the exfoliated cell blocks. The cell collection device can be used for collecting and preparing cell blocks of cast-off cells in samples such as pleural effusion, ascites, urine and sputum, has the advantages of efficient enrichment of a small number of cast-off cells and unsupported preparation of the cell blocks, facilitates later cell slicing, and improves the detection rate of later pathological detection.

Description

technical field [0001] The present application relates to the technical field of cell collection in body fluids, and more specifically, it relates to a cell block collection device and a cell block collection method. Background technique [0002] In the medical field, for the early diagnosis of lung cancer and liver cancer, at present, chest X-ray and chest CT scan are mainly used for initial diagnosis, followed by the acquisition, preparation and detection of tissue slices. However, the acquisition of tissue slices is often a damaged slice sample acquisition method, and the relevant tissue cells can only be obtained after wounds are made on the corresponding parts of the human body. Therefore, the subsequently developed non-destructive cell slice detection technology has been widely used by everyone. Non-destructive cell slicing is to extract the exfoliated cells of the target diagnostic site, and then slice and detect the extracted exfoliated cells after further processin...

AI Technical Summary

Problems solved by technology

Although this method can fix the exfoliated cells within a certain range, the enrichment effect of the cells is not good, especially the exfoliated cells in the exfoliated cell liquid collected at the beginning are not much, and the exfoliated cells with lesions will be less , when cell sectioning is performed, the obtained test sample may happen to not contain diseased exfoliated cells, which leads to the following: firstly, it is not conducive to the later stage of microscopic examination and pathological examination, resulting in inaccurate test results; secondly, if you want to To make the detection results more accurate, it is necessary to do more microscopic examination and detection of the same sample, resulting in a cumbersome detection process and high detection costs; Acquisition and enrichment of target cells of exfoliated cells in fluid and exfoliated cells in sputum), which in turn affects the accuracy of detection of exfoliated cells in the later stage

[0006] For the current various tumor detection, when the tumor is in the early stage and has not yet become cancerous, the amount of tumor cells in the form of exfoliated cells in the body fluid is relatively small, so the conventional method of collecting exfoliated cells is used to obtain In the sample, the amount of the target cells to be tested is very small, resulting in the final test result showing that the cell sample does not contain diseased cells, which is easy to cause false detection or missed detection.

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