Use of shikonin or its derivatives as an antifouling agent for preventing macrofouling organisms from adhering

A technology for fouling organisms and antifouling agents, applied in the field of natural products as antifouling agents, can solve problems such as the absence of shikonin, and achieve the effects of prolonging the antifouling period, good lipophilicity, and avoiding the phenomenon of violent release.

Active Publication Date: 2021-10-01
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no report of shikonin as marine antifouling agent

Method used

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  • Use of shikonin or its derivatives as an antifouling agent for preventing macrofouling organisms from adhering
  • Use of shikonin or its derivatives as an antifouling agent for preventing macrofouling organisms from adhering
  • Use of shikonin or its derivatives as an antifouling agent for preventing macrofouling organisms from adhering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Detection of Shikonin's Inhibitory Effect on Ocean Large Fouling Plants

[0037] The biological detection model of marine large-scale fouling plants adopts the common fouling plants in the ocean-Enteromorpha, which is a common fouling plant in my country's coastal areas. Put the collected Enteromorpha into fresh seawater to obtain spores, and dilute the spore liquid to a spore density of 1×10 6 cells mL -1 to spare. Using a six-hole plate (the inner diameter of the six-hole plate is 37mm) as the experimental equipment, the shikonin experimental group was set to 5, 10 and 50 μg mL -1 Concentration gradient (dissolved in methanol), 3 parallel samples in each group, 5mL system in each well of the six-well plate solution, including 500μL spore solution, 50μL sample solution, 4450μL membrane-filtered seawater (filtered through a 0.22μm membrane), set Membrane filtered seawater control group (ck1), 1% (volume fraction) methanol seawater solution control group (ck2)...

Embodiment 2

[0039] Example 2 Detection of the inhibitory effect of shikonin on marine macrofouling animals

[0040] A bioassay model of marine macrofouling fauna employs a common fouling shellfish - the emerald mussel. Emerald mussels secrete silk to attach to marine facilities, so the inhibition of the silk secretion of emerald mussels can prove that the compound has antifouling activity. Select emerald mussels (shell length is about 1cm), gently cut off the silk, clean and set aside. Dissolve shikonin in methanol with a concentration gradient setting of 0.5, 1, 5, 10 and 50 μg mL -1 , set membrane filter seawater control group (ck1), 1% methanol seawater solution control group (ck2). There were 10 parallel samples in each group. Take a clean 24-well plate, first add 1980 μL of membrane-filtered seawater (filtered through a 0.22 μm membrane) to the wells, and then add 20 μL of the prepared solution. Gently place the emerald mussels into the holes, one per hole. After standing for 24...

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Abstract

The invention discloses the use of shikonin or a derivative thereof as an antifouling agent for preventing the attachment of large fouling organisms. The natural product shikonin or its derivatives in comfrey can prevent large-scale fouling organisms, especially marine large-scale fouling organisms, from attaching to the surface of artificial facilities in the sea such as ships, buoys, docks, marine monitoring equipment or aquaculture equipment. A natural and environmentally friendly antifouling agent that can be used in antifouling coatings.

Description

technical field [0001] The invention relates to an antifouling agent, especially the use of a natural product extracted from comfrey as an antifouling agent. Background technique [0002] In the marine environment, the surface of artificial facilities will be attached by marine organisms. A large number of fouling organisms adhere to the surface of artificial facilities, which will cause serious harm to marine transportation, marine engineering, and mariculture. Large-scale marine fouling organisms such as enteromorpha and mussels adhere to the bottom of the ship, which will increase the resistance of the ship, reduce the speed of the ship, increase fuel consumption, and aggravate the emission of greenhouse gases. When the fouling area of ​​the ship is 50%, the operating cost will increase by 77% %. In addition, marine fouling organisms will accelerate the corrosion of metals and shorten the service life; they will also interfere with the signal transmission of marine moni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D5/16
CPCC09D5/1625
Inventor 苏培冯丹青柯才焕
Owner XIAMEN UNIV
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