Method for preparing magnetic nano amino compound, and nucleic acid extraction method

Abstract

The invention discloses a method for preparing a magnetic nano amino compound, and a nucleic acid extraction method. The method comprises the following steps: step 1, purifying diatomite for later use; after cleaning, putting the diatomite into a flask, and adding deionized water; standing after stirring, carrying out sample separation and centrifugation on a supernatant, collecting precipitates,and repeating the steps by using ethanol instead of deionized water so as to further remove ions and protein impurities; and finally, drying the purified diatomite in a drying box, and storing in a reagent bottle for use; step 2, carrying out diatomite magnetization treatment; drying magnetic diatomite DE@Fe3O4 in a drying box; step 3, loading APTES on the magnetic diatomite, firstly dropwise adding the APTES into an ethanol solution, and stirring by using a magnetic stirrer; then, adding the magnetic diatomite into an obtained solution, and collecting DE@Fe3O4@APTES by using a magnet; then respectively washing with ethanol and deionized water; and finally, drying the DE @Fe3O4 @APTES in a drying oven, and storing the DE @Fe3O4 @APTES in a reagent bottle for use.

Description

technical field [0001] The invention relates to the technical field of nucleic acid extraction, in particular to a method for preparing a magnetic nano-amino complex and a nucleic acid extraction method. Background technique [0002] Nucleic acid amplification tests (NAATs) have unmatched sensitivity and specificity and are considered a powerful star method for diagnosing infectious and disease-associated pathogens. Extraction of high-quality nucleic acids is critical for subsequent processes such as polymerase chain reaction (PCR) or isothermal amplification detection. Since the outbreak of the novel coronavirus (SARS-CoV-2)-infected pneumonia, the rapid marketing and clinical application of ordinary real-time fluorescent quantitative PCR nucleic acid detection reagents have played an important role in the clinical diagnosis of patients and the screening of suspected patients. Restrictions such as tediousness, time-consuming, and the need for centralized sample delivery ca...

AI Technical Summary

Problems solved by technology

The current method has many limitations, such as the need to use chaotropic reagents or other dangerous chemicals that may inhibit PCR enzyme activity; cumbersome sample handling steps and strict cleaning steps; laboratory equipment such as centrifuges, vacuums, and vortexers are required, Labor-intensive and time-consuming; complex fabrication steps including membrane / silicon-based substrate / micro- or nanoscale substrate mounting and fabrication, etc.

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