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Growth-promoting strain screening method based on high-throughput sequencing and application of method

A high-throughput, sequencing technology, applied in the field of soil microbial isolation, can solve the problem that the utilization potential of soil microbial resources cannot be fully utilized

Active Publication Date: 2020-10-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stress conditions are ubiquitous in terrestrial ecosystems, and unfavorable environmental conditions can not only inhibit the population of beneficial soil microorganisms, but also affect their functions, so that the utilization potential of soil microbial resources cannot be fully utilized

Method used

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  • Growth-promoting strain screening method based on high-throughput sequencing and application of method
  • Growth-promoting strain screening method based on high-throughput sequencing and application of method
  • Growth-promoting strain screening method based on high-throughput sequencing and application of method

Examples

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Effect test

Embodiment 1

[0033] Example 1 Screening method for growth-promoting fungal strains based on high-throughput sequencing

[0034] This example uses seven soil pHs as functional gradients to provide a screening method for growth-promoting fungal strains based on high-throughput sequencing. The specific experimental methods and results are as follows:

[0035] 1. Experimental method

[0036] A screening method for growth-promoting fungal strains based on high-throughput sequencing, comprising the following steps:

[0037] S1. Collect the soil 0-20cm above the ground from acidic red soil orchards in subtropical regions, pass it through a 2mm sieve after air-drying, and set aside.

[0038] S2. Adjust soil pH: take 7 parts of 2kg dry soil, add 1.0g / kg and 2.0g / kg sulfur powder to 2 parts of soil respectively, add 1.5g / kg, 3.0g / kg, 4.5g / kg to 4 parts of soil respectively , 7.5g / kg of calcium hydroxide, mix thoroughly, and the last part of soil is the original soil. 7 soils were cultivated in larg...

Embodiment 2

[0054] Example 2 Screening method for growth-promoting bacterial strains based on high-throughput sequencing

[0055] This embodiment uses seven soil pHs as functional gradients to provide a screening method for growth-promoting bacterial strains based on high-throughput sequencing. The specific experimental methods and results are as follows:

[0056] 1. Experimental method

[0057] A screening method for growth-promoting bacterial strains based on high-throughput sequencing, comprising the following steps:

[0058] S1. Collect the soil 0-20cm above the ground from acidic red soil orchards in subtropical regions, pass it through a 2mm sieve after air-drying, and set aside.

[0059] S2. Adjust soil pH: take 7 parts of 2kg dry soil, add 1.0g / kg and 2.0g / kg sulfur powder to 2 parts of soil respectively, add 1.5g / kg, 3.0g / kg, 4.5g / kg to 4 parts of soil respectively , 7.5g / kg of calcium hydroxide, mix thoroughly, and the last part of soil is the original soil. 7 soils were cult...

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Abstract

The invention discloses a growth-promoting strain screening method based on high-throughput sequencing and an application of the method. According to the method, an effective soil function screening gradient is constructed so as to form a gradient change difference between the growth of a test plant and the composition of a soil microbial community, and the community composition of the microorganisms in the soil is analyzed by combining a widely-used, low-cost and high-accuracy high-throughput sequencing technology; and by carrying out regression analysis through the absolute abundance of thedominant strain OTU and the biomass of the test plant, whether the dominant strain is a potential growth-promoting strain or not is judged, the dominant potential growth-promoting strain with high absolute abundance is separated, and a tie-back test is carried out to verify the growth-promoting effect of the dominant strain. According to the method, the growth-promoting strains are screened directly depending on the growth-promoting effect, and uncertainty and false positive of the strains screened according to functional genes and a functional selection culture medium in practical applicationcan be effectively avoided; and in addition, the method is clear in target, higher in success rate and wide in application prospect in screening growth-promoting strains.

Description

technical field [0001] The invention belongs to the technical field of soil microorganism separation. More specifically, it relates to a method for screening growth-promoting strains based on high-throughput sequencing and its application. Background technique [0002] Soil microorganisms are the most active and important part of the soil due to their large number and variety, and are known as the engine of the biogeochemical cycle process of the earth's key elements. For plants, some soil microorganisms can improve their nutritional status, enhance their tolerance to adversity, reduce the occurrence of diseases and insect pests, and promote plant growth and development. For example, mycorrhizal fungi. As a widely distributed soil fungus, arbuscular mycorrhizal fungi (AMF) can establish a mutually beneficial symbiotic relationship with more than 80% of plant roots on land. Once the symbiotic relationship is established, AMF can greatly improve the host plant's uptake of v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/04G16B40/30G16B40/20G16B25/00C12R1/645C12R1/01
CPCC12Q1/6869G16B25/00G16B40/20G16B40/30C12Q2535/122C12Q2531/113
Inventor 姚青冯曾威刘晓迪朱红惠
Owner SOUTH CHINA AGRI UNIV
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