Construction method of novel coronavirus N-His recombinant protein chip platform

Abstract

The invention provides a construction method of a novel coronavirus N-His recombinant protein chip platform. The construction method comprises the following steps: firstly, preparing N recombinant protein with good antigenicity through pET28a N plasmids; and then determining the optimal dilution of the first antibody and the second antibody by using the N recombinant protein, and finally determining the detection limit and the linear range of the chip by using the constructed chip to detect the serum subjected to gradient dilution, thereby completing the construction of the N gene recombinantprotein chip platform. Compared with an RT-PCR detection method, the chip has more stable detection performance, also has the characteristics of high throughput, microminiaturization and the like, isnot easily influenced by virus variation, and can more accurately and quickly realize large-batch screening of 2019-nCoV.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing a novel coronavirus N-His recombinant protein chip platform. Background technique [0002] In late December 2019, a group of patients with pneumonia of unknown cause appeared. After sequencing the samples of patients with pneumonia, a new type of coronavirus was discovered, which was subsequently named 2019-nCoV. The initial symptoms of patients with novel coronavirus (2019-nCoV) are mostly fever, fatigue and dry cough, and gradually develop severe manifestations such as dyspnea. Most patients have a good prognosis, and some severe cases may develop acute respiratory distress syndrome or septic shock, and even die. At present, there is no specific treatment for the disease, and the incubation period is long, with an average of 14 days. During the incubation period, it is still contagious, mainly through droplet transmission and contact transmission, and the...

AI Technical Summary

Problems solved by technology

At present, the main means of detecting 2019-nCoV in the whole country is RT-PCR. Although RT-PCR technology has the characteristics of high sensitivity and high specificity, the experiment has high requirements for the detection personnel and the experimental operation steps need frequent heating and cooling processes , requires expensive instruments, and may easily affect the sensitivity of RT-PCR detection due to possible mutations during virus transmission