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Preparation method and application of Heinz Sargassum fucoidan sulfate

A technology of Sargassum Fucoidan Sulfate and Sargassum Heinz, which is applied in the direction of antiviral agents, can solve the problems of unclear pharmacodynamic material basis and mechanism of action, achieve novel mechanism of action, ensure safety, and definite curative effect Effect

Active Publication Date: 2022-01-07
TIANJIN MEDICAL UNIV
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  • Description
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Problems solved by technology

Although some scholars have carried out chemical research on the polysaccharide components of Sargassum heinzii in recent years, due to the complexity of the polysaccharide structure and the limitation of analysis methods, the research on the polysaccharides of Sargassum heinzii is limited to the extraction process, The research on the composition of monosaccharides, the pharmacological activity is therefore limited to the research level of crude polysaccharides, and its pharmacodynamic material basis and mechanism of action are still unclear

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  • Preparation method and application of Heinz Sargassum fucoidan sulfate
  • Preparation method and application of Heinz Sargassum fucoidan sulfate
  • Preparation method and application of Heinz Sargassum fucoidan sulfate

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preparation example Construction

[0052] Prepare the preparation method of Heinz Sargassum fucoidan sulfate ester, the specific steps are as follows:

[0053] Step 1: Take the dried Sargassum henley medicinal material, reflux the hot water, concentrate the obtained solution or obtain an extract;

[0054] Step 2: adding ethanol to the extract for alcohol precipitation, collecting the alcohol precipitate and redissolving it in water, dialysis with deionized water, and lyophilizing the dialyzed internal solution to obtain the crude polysaccharide SHCP from Sargassum heinzii;

[0055] Step 3: Take Heinz Sargassum crude polysaccharide SHCP to make a crude polysaccharide solution, and obtain Heinz Sargassum fucoidan sulfate SHAP through multiple column chromatography purification;

[0056] Wherein, the specific purification steps in step three are as follows:

[0057] Step A prepares the crude polysaccharide SHCP solution of Sargassum Heinzii, passes through an anion exchange column, and uses H 2 O, 0.5mol / L NaCl,...

Embodiment 1

[0071] Example 1 Extraction, separation and purification of Heinz Sargassum fucoidan sulfate

[0072] The extraction and separation flow chart of Heinz Sargassum Fucoidan Sulfate is as follows figure 1 shown. Take the dried medicinal material of Sargassum Heinz, add 3 times the volume of absolute ethanol and degrease at room temperature for 24 hours, filter the dregs to remove the alcohol smell, add 30 times the quality of water, extract 3 times with hot water reflux, 2 hours each time, combine three times The filtrate was concentrated under reduced pressure. Add 98% ethanol to the concentrated solution to a final concentration of 80%. Depending on the situation, 95% ethanol or absolute ethanol can also be used, and put it at 4°C overnight for alcohol precipitation. The alcohol precipitate was redissolved in deionized water, dialyzed against deionized water with a 12000Da molecular weight dialysis bag, and the solution in the bag was collected, concentrated under reduced pre...

Embodiment 2

[0076] Example 2 Heinz Sargassum Fucoidan Sulphate SHAP-1 and SHAP-2 Structural Characteristic Analysis

[0077] 1. Homogeneity analysis and molecular weight determination

[0078] Take 5mg of pullulan series standard substances (molecular weights are 642, 337, 194, 107, 47.1, 21.1, 9.6, 6.1kD respectively), fully dissolve in 1 mL of ultrapure water, and prepare 5mg·mL respectively -1 Pullulan series standard solution, pass through 0.22μm filter membrane, set aside. Take 10 μL of the standard solution and inject it into a high-performance gel chromatograph (HPGPC), and use 0.02M CH 3 COONH 4 Elution, the flow rate is 0.6mL·min -1 , column temperature 40°C. Take the retention time A as the ordinate, and the molecular weight C as the abscissa to draw the standard curve A=-1.449C+19.868, R 2 = 0.9968. Take 5 mg of a single component sample of Sargassum polysaccharide from Heinz, fully dissolve it in 1 mL of ultrapure water, and configure it to 5 mg·mL -1 The sample solutio...

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Abstract

The invention provides Heinz sargassum fucoidan sulfate, which is composed of fucose and galactose and has a molecular weight of 4.0×10 5 -6.0×10 5 , Sargassum Heinzii Fucoidan Sulfate is extracted from Sargassum Heinzli; by extracting the crude polysaccharide from Sargassum Heinzii, and then purifying the crude polysaccharide through ion exchange chromatography and molecular sieve column for many times, it can be used for anti-herpes virus research Heinz Sargassum Fucosan Sulfate. This protocol can isolate natural polysaccharides with a novel antiviral mode of action, low toxicity and high efficiency, and safety and easy acceptance from marine medicinal resources Sargassum henzii. Research basis: Heinz Sargassum fucoidan sulfate can play an antiviral role by effectively inhibiting the adsorption of herpes virus to the surface of host cells. Its site of action is the cell surface and can act on the first step of virus infection. Entering cells, the possibility of causing toxic side effects is relatively small.

Description

technical field [0001] The invention belongs to the field of antiviral drugs, in particular to a preparation method and application of Heinz Sargassum fucoidan sulfate. Background technique [0002] Herpes simplex virus type II (HSV-2) can cause infectious diseases such as genital herpes and congenital herpetic encephalitis, and is also an important factor affecting human immunodeficiency virus (Human immunodeficiency virus, HIV) infection. The current treatment methods for HSV-2 infection mainly include vaccine immunization and antiviral drug therapy. Since 1990, the six clinical trials of HSV-2 vaccine development have all ended in failure. There are still many problems to be solved in HSV-2 vaccine research Further solving, it may take a long time to finally come out, therefore, drug therapy is still the main means for the treatment of HSV-2 infection at present. [0003] Due to the late start of the development of antiviral drugs, currently clinically effective drugs fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P31/22
CPCC08B37/006C08B37/0003A61P31/22
Inventor 董彩霞李轶邓菲
Owner TIANJIN MEDICAL UNIV
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