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A high compliance tissue engineered blood vessel preparation template and tissue engineered blood vessel

A tissue engineering and compliance technology, which is applied in the direction of blood vessels, tissue regeneration, and human tubular structure devices, can solve the problems of stimulating thrombus formation, prone to sliding, and intimal hyperplasia, so as to enhance anti-kink performance and reduce fiber exposure. , to ensure the effect of uniformity

Active Publication Date: 2022-08-05
领博生物科技(杭州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, the above-mentioned previous studies also reflected the following problems: First, some skeleton fibers in the tissue-engineered vascular lumen prepared after subcutaneous implantation using the above-mentioned templates were exposed, which would stimulate thrombus formation; The outer surface of the prepared tissue-engineered blood vessel is relatively smooth, and it is easy to slip after implantation, which reduces the integration rate of the blood vessel and the implantation site; third, the compliance of the blood vessel prepared by the above method is not good; fourth, the in vivo implantation intimal hyperplasia
[0005] Studies have found that one of the main reasons for intimal hyperplasia after implantation of artificial blood vessels is the mismatch between the compliance of artificial blood vessels and the compliance of natural blood vessels, which in turn causes the mechanical stimulation of the regenerated smooth muscle cells on the artificial blood vessels to be different from that of the natural blood vessels. The mechanical stimulation received does not match, causing excessive proliferation of smooth muscle cells, resulting in intimal hyperplasia

Method used

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  • A high compliance tissue engineered blood vessel preparation template and tissue engineered blood vessel
  • A high compliance tissue engineered blood vessel preparation template and tissue engineered blood vessel
  • A high compliance tissue engineered blood vessel preparation template and tissue engineered blood vessel

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preparation example Construction

[0050] The invention also discloses a preparation method of a skeleton main body, comprising the following steps:

[0051] Step 1, using conventional stent preparation technology to prepare a support layer;

[0052] In step 2, a degradable layer is prepared and arranged on the outside of the support layer by using a conventional stent preparation technology;

[0053] In step 3, the support layer is prepared again outside the degraded layer by using a conventional stent preparation technology;

[0054] Step 4. Repeat steps 2-3 until the desired number of layers is reached.

[0055] The invention also discloses a high compliance tissue engineering blood vessel, which is prepared by using the high compliance tissue engineering blood vessel preparation template as a template.

[0056] The highly compliant tissue engineering blood vessel includes an acellular matrix layer and a support fiber; the support fiber is the fiber in the template support layer, and the acellular matrix l...

Embodiment 1

[0074] The high compliance tissue engineering blood vessel preparation template in this embodiment includes a skeleton body and an inner core; the inner core is arranged inside the skeleton body, and the skeleton body includes a support layer and a degradation layer; the degradation layer is arranged on the Between two adjacent support layers; the degradation layer is 3 layers.

[0075] The template preparation process includes the following steps:

[0076] 1. A silicone tube with an outer diameter of 2 mm and an inner diameter of 1 mm is used as the inner core, and the outer diameter of the inner core determines the inner diameter of the prepared high compliance tissue engineered blood vessel.

[0077] 2. Using polycaprolactone (PCL) as raw material, using melt spinning technology to form a first support layer on the surface of the inner core, the fiber diameter in the first support layer is 20 μm, and the cross angle between fibers in the first support layer is 30°, and the...

Embodiment 2

[0089] The high compliance tissue engineering blood vessel preparation template in this embodiment includes a skeleton body and an inner core; the inner core is arranged inside the skeleton body, and the skeleton body includes a support layer and a degradation layer; the degradation layer is arranged on the Between two adjacent support layers; the degradation layer is 2 layers.

[0090] The template preparation process includes the following steps:

[0091] 1. A polyurethane tube with an outer diameter of 4 mm and an inner diameter of 2 mm is used as the inner core, and the outer diameter of the inner core determines the inner diameter of the prepared tissue engineered blood vessel.

[0092] 2. Using poly(lactide-caprolactone) copolymer (PLCL) as a raw material, a first support layer (2) is formed on the surface of the inner core by melt spinning technology, and the fiber diameter of the first support layer is 40 μm, The crossing angle between fibers of the first support laye...

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Abstract

The invention belongs to the field of tissue engineering, and in particular relates to a high-compliance tissue engineering blood vessel preparation template, comprising a skeleton main body (1) and an inner core (2); Inside, the outer diameter of the inner core (2) matches the inner diameter of the skeleton main body (1); the skeleton main body (1) main body includes a supporting layer (11) and a degradation layer (12) arranged alternately, and all the The inner and outer surfaces of the skeleton main body are both supporting layers (11); the degradable layer (12) is made of degradable materials; the high-compliance tissue engineering blood vessel preparation template is used for implantation under the skin of an animal or after tissue culture in vitro, and then extracted. After being separated from the inner core (2), a highly compliant tissue engineered blood vessel can be prepared after decellularization treatment. The beneficial effect of the present invention is that the tissue engineered blood vessel prepared by using the high compliance tissue engineered blood vessel preparation template has good compliance and can inhibit intimal hyperplasia.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a high-compliance tissue engineered blood vessel preparation template and a tissue engineered blood vessel. Background technique [0002] Vascular diseases are the most lethal diseases in the world, and are mainly caused by stenosis or blockage of blood vessels leading to reduced blood flow and nutrient deprivation, resulting in tissue or organ damage. It usually manifests as cerebrovascular disease, coronary heart disease, and peripheral arterial disease. According to the World Health Organization, by 2030, the number of people dying from cardiovascular-related diseases worldwide will increase to 23.3 million annually. Vascular transplantation is still the conventional method for the treatment of these diseases. At present, the use of the patient's own blood vessels (such as the great saphenous vein, bilateral internal thoracic arteries, radial arteries, etc.) is s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/36A61L27/58A61F2/06A61L27/50A61L27/18A61L27/24
CPCA61L27/507A61L27/50A61L27/58A61L27/3604A61F2/06A61L27/18A61L27/24A61L2430/40
Inventor 王恺张杰许鹏赟
Owner 领博生物科技(杭州)有限公司
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