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Detection method of wheat scab grain ZEN toxin

A technology of wheat scab and detection method, applied in the field of analytical chemistry, can solve the problem of high cost of detection and quantification of zearalenone, and achieve the effect of good recovery rate, convenient operation and high linear relationship

Inactive Publication Date: 2020-09-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the technical problem of high cost of detection and quantification of existing zearalenone, the present invention provides a detection method of ZEN toxin in wheat scab grains

Method used

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  • Detection method of wheat scab grain ZEN toxin
  • Detection method of wheat scab grain ZEN toxin
  • Detection method of wheat scab grain ZEN toxin

Examples

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Embodiment 1

[0047] A method for detecting ZEN toxin in wheat scab grains, comprising the steps of:

[0048] S1, prepare zearalenone standard solution;

[0049] Dissolve 5 mg of zearalenone standard substance in methanol and dilute to 100ml to prepare a 50ug / ml mother solution, then take 10ml of the mother solution to a 100ml volumetric flask to prepare a 5ug / ml working solution for later use, and dilute the mobile phase to 0.02 ug / ml, 0.05ug / ml, 0.1ug / ml, 0.5ug / ml, 1.0ug / ml concentration for use.

[0050] S2, preparation need testing solution

[0051] Wheat head blight grains are extracted, column purified, dried, diluted with mobile phase, passed through an organic filter, and the filtrate collected is the test solution, as follows:

[0052] Weigh 25g of ground wheat scab grains, add 100ml of acetonitrile-water mixed solvent, ultrasonicate for 30min, let stand and filter, absorb 8mL of filtrate, transfer 80ul of acetic acid to Mycosep#226 multifunctional purification column tube, colle...

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Abstract

The invention relates to the technical field of analytical chemistry, in particular to a detection method of wheat scab grain ZEN toxin. The method comprises the following steps: preparing a zearalenone standard substance solution; preparing a test solution, extracting wheat scab seeds, performing column purification, performing drying, performing diluting with a mobile phase, performing filteringwith an organic filter membrane, and collecting the filtrate, thereby obtaining the test solution; carrying out the HPLC-DAD detection, wherein chromatographic conditions are as follows: a chromatographic column is a C18 chromatographic column with the size of 250 mm * 4.6 mm and the size of 5 [mu]m, a mobile phase is a water-acetonitrile-methanol solution, and the volume ratio of water to acetonitrile to methanol is (45-46): (45-46): (8-10), the flow rate is 0.8 to 1.0 ml / min, the detection wavelength lambda is equal to 270nm, the column temperature is 38-40 DEG C, and the sample size is 18to 20 [mu]l; measuring the peak area of the zearalenone standard substance solution, drawing a standard curve, calculating a regression equation, measuring the peak area of the test solution, and obtaining the content of the ZEN toxin in the wheat scab grains through the standard regression equation. The detection method is good in recovery rate, high in linear relation, convenient to operate andeasy to popularize.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for detecting ZEN toxin in wheat scab grains. Background technique [0002] Zearalenone, also known as F2 toxin, white crystals, molecular formula C18H22O5, chemical name 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-β-lycine- Lactone, relative molecular mass 318.36, melting point 164-165°C, insoluble in water and carbon tetraoxide, slightly soluble in petroleum ether, soluble in alkaline aqueous solution, ethyl acetate and alcohols. ZEN methanol solution is blue-green under ultraviolet light irradiation, and has good heat resistance. [0003] ZEN toxin is mainly produced by Fusarium fungus, which was first isolated by Stob in 1962 from moldy corn. ZEN widely pollutes moldy corn, barley, wheat and other grains and related products, and ZEN contamination in feed raw materials and feed ingredients is common. ZEN toxin contamination is also common in daily necessit...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8675G01N2030/065
Inventor 陈璨卢杰梁坤司红起马传喜
Owner ANHUI AGRICULTURAL UNIVERSITY
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