Method for detecting new coronavirus SARS-CoV-2 N protein by adopting nucleic acid aptamer

Abstract

The invention discloses a method for detecting a new coronavirus SARS-CoV-2 N protein by adopting a nucleic acid aptamer. The aptamer is N Aptamer 1, N Aptamer 2 or N Aptamer 3, can be specifically combined with SARS-CoV-2 virus N protein, and can also be used for specifically detecting N protein diluted in human serum and N protein in a protein electrophoresis sample. By means of the mode, nucleic acid extraction or antibody detection is not needed, meanwhile, the method has the advantages of being high in affinity with target molecules, high in specificity, easy to prepare, low in immunogenicity and toxicity, stable in chemical structure and the like, and conditions are created for early detection of 2019 SARS-CoV-2 pathogens.

Description

technical field [0001] The invention relates to the technical field of biomedical applications, in particular to a method for detecting the N protein of the new coronavirus SARS-CoV-2 using a nucleic acid aptamer. Background technique [0002] The novel coronavirus, with its high infectivity and strong pathogenicity, quickly caused a pandemic of new coronary pneumonia around the world, posing a serious threat to human health and social economy. Rapid and accurate diagnosis of suspected cases, effective isolation of infected persons and active treatment are important foundations for epidemic prevention and control. [0003] At present, the routine methods used in virological diagnosis include: 1. Virus isolation and culture (gold standard for virus characteristics, morphology, staining and biochemical identification); 3. Molecular biology techniques (nucleic acid molecular hybridization, PCR, nucleic acid sequencing, etc.). [0004] However, each detection method has its di...

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Problems solved by technology

[0004] However, each detection method has its disadvantages: 1. Virus isolation and cultivation: The isolation, cultivation and identification of viruses is the gold standard for virus diagnosis, but its methods are complicated, require high technology and facilities, and take a long time. There are difficulties in widespread implementation; 2. Immunological techniques: At present, the new clinical guidelines point out that the detection of new coronavirus-specific IgM in patients begins 3 to 5 days after onset

IgG is even later, starting to appear on 10-21 days, and it will increase 4 times in the recovery period, so this method can only be used for the diagnosis of the middle and late stages of the disease, and due to the methodological reasons of antibody detection, the detection results are accurate 3. Molecular biology technology-nucleic acid detection: According to reports, the false negative rate of new coronavirus nucleic acid detection can reach as high as 30-50%. At the same time, kit detection The results are not only related to the quality of the kit and the upper and lower limits of detection, but are also affected by factors such as the characteristics of the new coronavirus itself, sampling sites, sampling volume, transportation and storage links, as well as laboratory testing conditions and personnel operations.

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