Preparation method of small and dense low-density lipoprotein cholesterol detection kit

A low-density lipoprotein and detection kit technology, applied in the field of medical detection, can solve the problems of unsatisfactory detection sensitivity and specificity, inconvenient use, cumbersome operation, etc., and achieve fast and sensitive determination, easy operation and good stability

Pending Publication Date: 2020-09-22
HONGKUI BIOLOGICAL CHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the existing small and dense low-density lipoprotein cholesterol detection kits have the problems of cumbersome operation, long time consu...

Method used

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  • Preparation method of small and dense low-density lipoprotein cholesterol detection kit
  • Preparation method of small and dense low-density lipoprotein cholesterol detection kit
  • Preparation method of small and dense low-density lipoprotein cholesterol detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method of a small and dense low-density lipoprotein cholesterol detection kit, comprising the following steps:

[0022] S1. Obtain the components required for reagent R1, MES buffer solution-100mmol / L cholesterol esterase 1.6KU / L cholesterol oxidase 0.6KU / L phospholipase 2.7KU / L catalase-1.2KU / L Toos 2mmol / L The agar gel of 0.3g / L; Concentration is the concentration of the polyethylene glycol (PEG) phosphotungstic acid of 1g / L is 4.5g / L;

[0023] S2. Obtain the components required for reagent R2, MES buffer solution II 100mmol / L peroxidase II 5KU / L 4-aminoantipyrine 4mmol / L sodium azide 0.05%;

[0024] S3, preparing reagent R1, dissolving the raw material of R1 in distilled water or double distilled water, mixing evenly to a constant volume;

[0025] S4. Prepare reagent R2. Dissolve 4-aminoantipyrine in R2 in MES buffer 2, mix well, then add peroxidase 2 and sodium azide to the solution, stir well, mix well, set The pH of both reagent R1 and reagent R2 i...

Embodiment 1

[0059] Table 5 Example 1 kit linearity test

[0060] table 5:

[0061]

[0062]

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Abstract

The invention relates to the technical field of medical detection, and discloses a preparation method of a small and dense low-density lipoprotein cholesterol detection kit. The method comprises the following steps of S1, obtaining components required by a reagent R1, wherein the components include 100 mmol/L of an MES buffer solution I, 1.6 KU/L of cholesterol esterase, 0.6 KU/L of cholesterol oxidase, 2.7 KU/L of phospholipase, 1.2 KU/L of catalase I, 2 mmol/L of Toos and 0.3 g/L of agar gel, the concentration of polyethylene glycol (PEG) is 1 g/L, and the concentration of phosphotungstic acid is 4.5 g/L; and S2, obtaining components required by a reagent R2, wherein the components include 100 mmol/L of an MES buffer solution II, 5 KU/L of peroxidase II, 4 mmol/L of 4-aminoantipyrine and0.05% of sodium azide. The method is used for quantitatively determining the content of small and dense low-density lipoprotein cholesterol in human serum in vitro. The peroxidase used in the kit improves the sensitivity and specificity of detection.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a preparation method of a small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Elevated low-density lipoprotein cholesterol (LDL-C) level is an independent risk factor for atherosclerosis [1], and sdLDL-C, as the main component of LDL, is relatively different from large and light low-density lipoprotein (L LDL ), sdLDL-C is more atherogenic due to its high invasiveness to the vessel wall, low affinity for LDL receptors, longer plasma half-life, and low tolerance to oxidative stress hardening. [0003] Recent studies have shown that sdLDL-C is an important marker of cardiovascular disease and can predict the risk of coronary heart disease. Relevant clinical or laboratory testing methods are: enzymatic method. [0004] The first step: under the action of cholesterol lipase (CHE), cholesterol oxidase (CO), phospholipase and ca...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/44C12Q1/42C12Q1/30C12Q1/28C12Q1/26
CPCC12Q1/60C12Q1/44C12Q1/42C12Q1/30C12Q1/28C12Q1/26G01N2333/916G01N2333/918G01N2333/904G01N2333/908C12Q2326/96
Inventor 王保全高洪元方洪帅崔学儒邢瑞王艳丽
Owner HONGKUI BIOLOGICAL CHINA CO LTD
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