Preparation method of small and dense low-density lipoprotein cholesterol detection kit

Abstract

The invention relates to the technical field of medical detection, and discloses a preparation method of a small and dense low-density lipoprotein cholesterol detection kit. The method comprises the following steps of S1, obtaining components required by a reagent R1, wherein the components include 100 mmol / L of an MES buffer solution I, 1.6 KU / L of cholesterol esterase, 0.6 KU / L of cholesterol oxidase, 2.7 KU / L of phospholipase, 1.2 KU / L of catalase I, 2 mmol / L of Toos and 0.3 g / L of agar gel, the concentration of polyethylene glycol (PEG) is 1 g / L, and the concentration of phosphotungstic acid is 4.5 g / L; and S2, obtaining components required by a reagent R2, wherein the components include 100 mmol / L of an MES buffer solution II, 5 KU / L of peroxidase II, 4 mmol / L of 4-aminoantipyrine and0.05% of sodium azide. The method is used for quantitatively determining the content of small and dense low-density lipoprotein cholesterol in human serum in vitro. The peroxidase used in the kit improves the sensitivity and specificity of detection.

Description

technical field [0001] The invention relates to the technical field of medical detection, in particular to a preparation method of a small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Elevated low-density lipoprotein cholesterol (LDL-C) level is an independent risk factor for atherosclerosis [1], and sdLDL-C, as the main component of LDL, is relatively different from large and light low-density lipoprotein (L LDL ), sdLDL-C is more atherogenic due to its high invasiveness to the vessel wall, low affinity for LDL receptors, longer plasma half-life, and low tolerance to oxidative stress hardening. [0003] Recent studies have shown that sdLDL-C is an important marker of cardiovascular disease and can predict the risk of coronary heart disease. Relevant clinical or laboratory testing methods are: enzymatic method. [0004] The first step: under the action of cholesterol lipase (CHE), cholesterol oxidase (CO), phospholipase and ca...

AI Technical Summary

Problems solved by technology

[0006] However, the existing small and dense low-density lipoprotein cholesterol detection kits have the problems of cumbersome operation, long time consumption, excessive waste of resources, and unsatisfactory detection sensitivity and specificity in the process of use, which is not convenient for people to use.

Images