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Composition for antioxidation, anti-inflammation, or osteoclast differentiation inhibition

A composition and anti-oxidation technology, applied in the functions of food ingredients, medical preparations containing active ingredients, applications, etc., can solve problems such as effective research or reporting of osteoclast differentiation inhibition ability

Active Publication Date: 2020-09-11
NATURALENDO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, there is no composite extract of Baishouwu, Han Dieshuan, Korean Angelica or its fractions effective for anti-oxidation, anti-inflammatory effect and osteoclast differentiation inhibition ability by inhibiting nitrite (NO) production. research or report

Method used

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  • Composition for antioxidation, anti-inflammation, or osteoclast differentiation inhibition
  • Composition for antioxidation, anti-inflammation, or osteoclast differentiation inhibition
  • Composition for antioxidation, anti-inflammation, or osteoclast differentiation inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Preparation of compound hot water extracts of Baishouwu, Han Dieshuan, and Korean Angelica

[0056] 125g of Baishouwu, 125g of Hanxuan and 135g of After the Angelica sinensis, it was extracted by refluxing hot water for 8 hours with water corresponding to 10 times (w / v, 1 / 10) the weight of the mixed crude drug. After that, the filtered filtrate was concentrated under reduced pressure to 20 to 40 brix by using an ultrafiltration membrane with a molecular weight cut-off of 10 μm, and then freeze-dried to obtain 172 g of Baishouwu, Han Dieshuan, and Korean Angelica in powder form. Complex hot water extract.

Embodiment 2

[0057]Embodiment 2: Preparation of water and organic solvent fractions

[0058] 2-1. Hexane fraction

[0059] 100g of the compound hot water extracts of Radix Polygoni Multiflori, Han Diessus, and Angelica sinensis prepared in the above steps were completely suspended in 500mL of water, and 500mL of hexane was added to repeatedly extract 4 times, and the hexane fraction was collected and passed through The solvent was completely removed by concentration under reduced pressure to obtain 0.5 g of a hexane fraction.

[0060] 2-2. Ethyl acetate fraction

[0061] After repeating extraction four times by adding 500 mL of ethyl acetate to the remaining layer (lower layer) except for the hexane fraction in 2-1 above, the ethyl acetate fraction was collected and concentrated under reduced pressure to completely remove the solvent to obtain 3.2 g of ethyl acetate fractions were obtained.

[0062] 2-3. Butanol fraction

[0063] After adding 500 mL of butanol to the remaining layer (l...

Embodiment 3

[0066] Example 3: Determination of 1,1-diphenyl-2-picrylhydrazine free radical scavenging activity

[0067] The antioxidant activity of radical scavenging ability was measured by confirming the ability to reduce or neutralize 1,1-diphenyl-2-picrylhydrazine group as a radical, that is, the oxidation inhibitory activity. 1, 1-diphenyl-2-picrylhydrazine is a relatively stable free radical in deep purple, which is reduced and decolorized by the sample, so it is used to measure the antioxidant activity. Specifically, 180 μl of 100 μM 1,1-diphenyl-2-picrylhydrazine solution was added to 20 μl of complex hot water extracts and fractions of Radix Polygoni Multiflori, Dipseuthes sativa, and Angelica sinensis, and reacted in dark vision for 30 Absorbance was measured at 517 nm after 1 minute, thereby confirming the concentration-dependent 1,1-diphenyl-2-picrylhydrazyl radical scavenging ability. Vitamin C was used as a positive control comparison substance, and the radical scavenging a...

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Abstract

The present invention relates to a composition for antioxidation, anti-inflammation, or osteoclast differentiation inhibition and a composition for arthritis treatment, each of the compositions containing a fraction of a complex extract of Cynanchum wilfordii, Phlomis umbrosa, and Angelica gigas as an active ingredient. The compositions of the present invention can be effectively used for osteoclast differentiation inhibition, antioxidation, or anti-inflammation, and arthritis treatment.

Description

technical field [0001] This application claims priority to Korean Patent Application No. 10-2017-0141519 filed with the Korean Patent Office on October 27, 2017, and the disclosures of the above patent application are incorporated herein by reference. [0002] The present invention relates to an anti-oxidation, anti-inflammation or composition for inhibiting osteoclast differentiation comprising the compound hot water extract fractions of Radix Polygoni Multiflori, Dipsacus and Angelicae sinensis as active ingredients. Background technique [0003] Osteoclasts are cells that resorb bone, are activated by various factors in the body, and maintain good bone quality by balancing with osteoblasts that produce bone. An imbalance between osteoclasts and osteoblasts induces various bone diseases, and in particular, an increase in osteoclasts induces not only osteoporosis but also rheumatoid arthritis, periodontitis, and the like. Osteoclasts originate from hematopoietic stem cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61K36/53A61K36/232A23L33/105
CPCA23L33/105A61K36/232A61K36/27A61K36/53A61K2300/00A61K2236/39A23V2002/00A23V2200/306A61K36/24A61K2236/331
Inventor 金载洙李权泽李镕旭
Owner NATURALENDO TECH
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