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Method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells

A tetraploid and chimera technology, applied in plant cells, botanical equipment and methods, horticultural methods, etc., can solve problems such as induction failure, achieve the effect of improving doubling efficiency and shortening the breeding cycle

Active Publication Date: 2020-09-11
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chimerism is a common problem encountered in polyploid breeding at present. The doubled tissue or cells cannot be separated from the chimera in time, and will be surrounded by vigorously growing normal diploid cells, and eventually disappear, and the plant returns to its original state. ploidy, resulting in induction failure

Method used

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  • Method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells
  • Method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells
  • Method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells

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Embodiment Construction

[0040] The implementation of the present invention will be described in detail below with examples, so as to fully understand and implement the implementation process of how the present invention uses technical means to solve technical problems and achieve technical effects.

[0041] The invention discloses a method for efficiently obtaining tetraploid magnolia bark plants and cells without chimera, comprising the following steps:

[0042] Step 1. Prepare embryogenic cell mass induction material with a pore size of 200-100 μm:

[0043] Such as figure 1 As shown, about 500 mg of Magnolia officinalis embryogenic callus that was subcultured every 2 weeks and well developed was picked and put into 20 ml of M2 liquid medium (without 2,4-D ) into a 50ml Erlenmeyer flask; add a magnetic stirrer (size 7mm*30mm) cleaned with 75% alcohol, and place it on an IKA C-MAG HS7 magnetic stirrer at 0°C and 1250rpm to break and disperse the cell mass for 10 minutes Remove them later and pass t...

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Abstract

The invention discloses a method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells. The method comprises the following steps of preparing embryogenic cellinduction materials of which the pore diameter is 200-100[mu]m, treating the embryogenic cells of 200-100[mu]m with colchicine of different concentrations, enabling the treated embryogenic cells to grow and plants to regrow, identifying tetraploid cells and plants, and domesticating and transplanting cortex magnoliae officinalis tetraploid cell differentiation embryos, somatic embryo differentiation plants and tetraploid cortex magnoliae officinalis tissue culture seedlings. According to the method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells, the redoubling efficiency of cortex magnoliae officinalis diploid can be notably improved, a key technology difficult problem that in the polyploid breeding process, chimeras are easy to generate, is solved, the polyploid breeding efficiency is greatly improved, and large-scale application of a polyploid breeding technique to generate the chimera-free tetraploid cortex magnoliae officinalis plants and cells is possible to realize.

Description

technical field [0001] The invention belongs to the technical field of biological breeding, and in particular relates to a method for efficiently obtaining tetraploid magnolia bark plants and cells without chimera. Background technique [0002] Magnolia officinalis Rehd. et Wils. is a plant of the genus Magnolia in the family Magnoliaceae. Magnolia officinalis bark, root bark, branch bark and flowers and fruits can be used as medicine. Magnolia officinalis bark is a traditional Chinese medicine with a history of more than 2,000 years of medicinal use in my country. The main active ingredients of magnolia bark include magnolol, honokiol, magnolol, volatile oil, β-cineole, etc., wherein the main active ingredients magnolol (C18H18O2) and honokiol (C18H18O2) have antitumor , Antibacterial, anti-ulcer, anti-depressant and anti-caries effects. Magnolia officinalis not only has important medicinal value, but also has high ornamental value. Due to its medicinal value, wild Magn...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04C12N15/01
CPCA01H4/001A01H4/005A01H4/008C12N15/01C12N5/04
Inventor 林亮贾艳霞高彦粉马俊超郁步竹徐倩李唯奇
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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