One-measurement double-index rapid qualitative and quantitative detection method of Guanxinkang granules for treatment of cardiovascular and cerebrovascular diseases

A quantitative detection method, the technology of Guanxinkang, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not checking the quality of Guanxinkang granules, shorten the identification time, reduce costs, and expand the scope of popularization and promotion Effect

Active Publication Date: 2020-09-08
承德燕峰药业有限责任公司
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  • Application Information

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Problems solved by technology

[0004] At present, there is no literature report on the quality of Guanxinkang Granules, which is simple, fast, one plate with two medicinal materials, and one test with two indicators.

Method used

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  • One-measurement double-index rapid qualitative and quantitative detection method of Guanxinkang granules for treatment of cardiovascular and cerebrovascular diseases
  • One-measurement double-index rapid qualitative and quantitative detection method of Guanxinkang granules for treatment of cardiovascular and cerebrovascular diseases
  • One-measurement double-index rapid qualitative and quantitative detection method of Guanxinkang granules for treatment of cardiovascular and cerebrovascular diseases

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Experimental program
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Embodiment

[0051] (1) Rapid TLC identification method

[0052] ①Take 2.0g of Guanxinkang granule powder, add 5ml of methanol, sonicate for 10 minutes, centrifuge, and use the supernatant as the test solution; take 0.2g of the reference medicinal material of Radix Paeoniae Rubra and 0.3g of the reference medicinal material of Salvia miltiorrhiza, add 2ml of methanol respectively , sonicated for 10 minutes, centrifuged, and the supernatants were used as the respective control medicinal solution; draw 3-5 μl of the control medicinal solution, 8-10 μl of the test solution, and spot on the same silica gel GF 254 On a thin-layer board, use ethyl acetate-butanone-formic acid-water at a volume ratio of 15:2:1:0.3 as a developer, develop, take out, dry with hot air, and spray with 5% vanilla at a volume ratio of 1:6 The mixed solution of aldehyde sulfuric acid solution-ethanol was heated at 105°C until the spots were clearly colored, and then observed, in the chromatogram of the test product, the...

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Abstract

The invention relates to a one-measurement double-index rapid qualitative and quantitative detection method of Guanxinkang granules for treatment of cardiovascular and cerebrovascular diseases. The method is characterized in that thin-layer chromatography is adopted, the root of red-rooted salvia, root of common peony, the rhizome of chuanxiong and rosewood are identified on two thin-layer plateswith the same test solution, and two traditional Chinese medicinal materials are identified on each thin-layer plate, wherein red spots of the root of red-rooted salvia and bright green fluorescent spots of rosewood after fluorescence enhancement with a 10% sulfuric acid ethanol solution are reported for the first time; the content of salvianic acid A sodium and paeoniflorin in a sample is simultaneously determined at 203nm by adopting common isocratic elution and taking acetonitrile-methanol-0. 1% phosphoric acid with the volume ratio of 7: 4.5: 88.5 as a mobile phase, and the peak retentiontime of salvianic acid A sodium is about 5 minutes, and the peak retention time of paeoniflorin is about 30 minutes. The method is simple, convenient, quick, accurate, reproducible and easy to popularize, the detection efficiency is effectively improved, the detection cost is reduced, and the environmental pollution is reduced.

Description

technical field [0001] The invention relates to a rapid qualitative and quantitative detection method of Guanxinkang Granules with one measurement and two indexes. The content includes rapid thin-layer identification and one-test double-indicator content determination, which belongs to the field of thin-layer identification and content determination. Background technique [0002] Guanxinkang Granules (sucrose-free type) are obtained by changing the drug specifications and excipients from the sucrose-containing type. On the basis of the same preparation process, the original specification is 10g per bag, one bag at a time, three times a day, and changed to no After the sucrose type, 6g per bag, one bag at a time, 3 times a day. The daily dose of crude drug remains unchanged, but there is no need to take a large amount of sucrose, and the dose is reduced, which improves the safety of medication for middle-aged and elderly people and diabetics. [0003] Guanxinkang Granules (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95G01N30/02G01N30/06G01N30/34G01N30/74G01N30/89
CPCG01N30/90G01N30/94G01N30/95G01N30/02G01N30/06G01N30/34G01N30/74G01N30/89
Inventor 张鹏徐达王立伟金艳敏申玉龙安丽娜蔡金凯韩治忠
Owner 承德燕峰药业有限责任公司
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