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Standard detection sequence for DNA bar code of Leuciscus waleckii and application of standard detection sequence

A standard detection technology for crocodile, applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problem of no data, etc., and achieve accurate identification, high repeatability, and accurate detection Effect

Active Publication Date: 2017-11-17
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the endemism of many fishes in our country, there is not enough relevant data

Method used

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  • Standard detection sequence for DNA bar code of Leuciscus waleckii and application of standard detection sequence
  • Standard detection sequence for DNA bar code of Leuciscus waleckii and application of standard detection sequence

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Effect test

specific Embodiment approach 1

[0024] Specific embodiment 1: In this embodiment, the standard detection sequence of the DNA barcode of the crocodile veschina is shown in SEQ ID NO: 1 in the sequence listing.

specific Embodiment approach 2

[0025] Embodiment 2: This embodiment differs from Embodiment 1 in that: the standard detection sequence of the DNA barcode of the crocodile crocodile is the COI gene. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Specific embodiment three: The difference between this embodiment and specific embodiment one is: the primers used to detect the standard detection sequence of the barcode DNA barcode of the crocodile, including the forward primer 5'-TCAACCAACCACAAAGACATTGGCAC-3' and the reverse primer 5' -TAGACTTCTGGGTGGCCAAAGAATCA-3'. Others are the same as in the first embodiment.

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PUM

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Abstract

The invention relates to a standard detection sequence for a DNA bar code of Leuciscus waleckii and application of the standard detection sequence. The invention aims at providing the standard detection sequence for the DNA bar code of the Leuciscus waleckii and application of the standard detection sequence in species identification. The standard detection sequence is represented by SEQ ID NO:1 in a sequence table. By utilizing the standard detection sequence for the DNA bar code of the Leuciscus waleckii, the molecular identification of the Leuciscus waleckii can be realized, and the identification time can be effectively shortened. The standard detection sequence is used for identifying the Leuciscus waleckii.

Description

technical field [0001] The invention relates to a standard detection sequence of a DNA barcode of crocodile veschii and an application thereof. Background technique [0002] Leuciscus waleckii (Leuciscus waleckii), also known as Northeast Leuciscus waleckii, is commonly known as Huazi fish, slippery fish, and white fish. Smaller cold water economic fish. Long, fusiform, flattened sides, round abdomen, without skin ribs; the mouth is located at the end, the mouth row is inclined, the upper jaw is inclined, and it is longer than the lower jaw; the lips are thin, without beards, and without horny edges; the snout is blunt and slightly raised , snout length slightly longer than eye diameter; medium-sized eyes, short gill rakers, medium-large scales, complete lateral line, slightly curved ventrally, extending back to the central axis of caudal peduncle; dorsal fin without hard spines; body back and upper side grayish black , belly silvery white, paired and anal fins yellow, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2563/185
Inventor 孙博苏宝锋常玉梅梁利群
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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