A kind of indole alkaloids in traditional Chinese medicine Herba Spondyloides and its extraction and purification method and application

A purification method and alkaloid technology, applied in the field of pharmacy, can solve problems such as no hemostasis research reports, achieve the effect of shortening plasma recalcification time and solving thrombus formation

Active Publication Date: 2021-09-07
贵州中医药大学
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Literature research shows that there are not many reports on the chemical constituents and other related researches of S. [12] For the determination of the total flavonoid content of C. spondyloides, Zhao Limin et al. separated two trace phenolic glycosides--isoquercitrin and milk vetch from the n-butanol part of the water extract of C. spondyloides, and Zhang Mengke isolated 45 compounds from them. , Chen Qiong isolated 17 compounds from it, and Wang Heying et al. isolated 13 compounds from it, but there is no relevant research report on its hemostasis

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  • A kind of indole alkaloids in traditional Chinese medicine Herba Spondyloides and its extraction and purification method and application
  • A kind of indole alkaloids in traditional Chinese medicine Herba Spondyloides and its extraction and purification method and application
  • A kind of indole alkaloids in traditional Chinese medicine Herba Spondyloides and its extraction and purification method and application

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Experimental program
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Effect test

Embodiment 1

[0060] Extraction and purification method of indole alkaloids

[0061] After 9.3kg of dried C. chinensis whole herb was crushed into coarse powder, reflux extracted with 95% industrial ethanol for 3 times, each time for 2 hours, concentrated under reduced pressure, and recovered the solvent to obtain 1756g of alcohol extract; mix it 1:1 After being suspended in water and dispersed, sequentially extract 1:1 with petroleum ether, chloroform, ethyl acetate, and n-butanol, each polar solvent is extracted 6 times, and each extract is combined separately, and the solvent is recovered by a rotary evaporator , Obtain petroleum ether part extract (286.6g), chloroform part extract (244.9g), ethyl acetate part extract (188.6g), n-butanol part extract (205.1g) and water part.

[0062] The extract from the petroleum ether part is dissolved in a solvent, and then mixed with silica gel (100-200 mesh), the sample is dry-applied to a silica gel column, and the silica gel column chromatography ...

Embodiment 2

[0087] Extraction and purification method of indole alkaloids

[0088] After taking 7kg of dried whole herb of Herba Sinensis and crushing it into coarse powder, use 8% industrial ethanol to reflux extract twice, each time for 3 hours, concentrate under reduced pressure, recover the solvent, and obtain 1300g of alcohol extract; mix it 1:1 After being suspended in water and dispersed, sequentially extract 1:1 with petroleum ether, chloroform, ethyl acetate, and n-butanol, each polar solvent is extracted 8 times, and each extract is combined separately, and the solvent is recovered by a rotary evaporator , respectively obtain petroleum ether part extractum 210g, chloroform part extractum 180g, ethyl acetate part extractum 140g, n-butanol part extractum 150g and water part;

[0089] The extract from the petroleum ether part was dissolved in a solvent and mixed with silica gel, then applied to a silica gel column in a dry method, eluted with a 20:1-0:1 chromatographic petroleum et...

Embodiment 3

[0113] Extraction and purification method of indole alkaloids

[0114] After crushing 12kg of dried C. chinensis into coarse powder, use 98% industrial ethanol to reflux extract twice, each time for 1 hour, concentrate under reduced pressure, recover the solvent, and obtain 2300g of alcohol extract; mix it 1:1 After being suspended in water and dispersed, sequentially extract 1:1 with petroleum ether, chloroform, ethyl acetate, and n-butanol, each polar solvent is extracted 4 times, and each extract is combined separately, and the solvent is recovered by a rotary evaporator , obtain petroleum ether part extractum 360g, chloroform part extractum 320g, ethyl acetate part extractum 240g, n-butanol part extractum 265g and water part;

[0115] The extract from the petroleum ether part was dissolved in a solvent and mixed with silica gel, then applied to a silica gel column in a dry method, eluted with a 20:1-0:1 chromatographic petroleum ether-ethyl acetate gradient through the sil...

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Abstract

The present invention relates to an indole alkaloid in the traditional Chinese medicine Herba Sinensis and its extraction and purification method and application. The researches on the hemostatic activity, the separation of the chemical components of the hemostatic active part and the activity screening of the hemostatic material basis, etc. Through these studies, we have preliminarily grasped the hemostatic efficacy of S. sanctana, analyzed the chemical composition of the hemostatic active parts of S. sanctana, and determined the hemostatic material basis of S. sanctana. On this basis, we can scientifically evaluate the efficacy Folk medicine basis, at the same time provide a practical basis for the research and development of new hemostatic drugs, and the quality evaluation standards of S. sanguineia; provide a scientific basis for the development and utilization of S. sanguineia resources.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to an indole alkaloid in the traditional Chinese medicine Herba Spontifolia and its extraction and purification method and application. Background technique [0002] Hemostatic drugs are drugs that accelerate blood coagulation, reduce the permeability of capillaries, or promote the contraction of blood vessel ends to stop bleeding; they can be used to treat bleeding or bleeding diseases caused by various reasons. Hemostatic drugs are not only widely used clinically, but also widely used in daily life, which has important clinical significance and research value. [0003] According to relevant statistics, the amount of hemostatic drugs used in Chinese hospitals has basically maintained a relatively stable upward trend in the level of medication; if only Western medicine and Western medicine are used, it will often lead to hypercoagulable state and bring dangerous consequences. Such western m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/42A61P7/04A61K31/405
CPCA61P7/04C07B2200/13C07D209/42
Inventor 杨武德郭正红陶小艳於祥隋怡
Owner 贵州中医药大学
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