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Nervate twayblade herb extracts and application thereof

A technology of blood serum and extract, applied in the field of blood serum extract, can solve the problem of not seeing active ingredients in effective parts of blood serum, and achieve good hemostatic effect

Inactive Publication Date: 2013-04-17
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no relevant reports on the effective parts and active ingredients of the serum

Method used

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  • Nervate twayblade herb extracts and application thereof
  • Nervate twayblade herb extracts and application thereof
  • Nervate twayblade herb extracts and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 See the preparation of serum ethanol extract

[0016] Take the serum medicinal material, crush it, add 85% ethanol to reflux extraction twice, each time for 3 hours, add 10 times the amount of medicinal material (W / V) ethanol each time, filter while it is hot, combine the filtrate, and recover until there is no alcohol smell After drying, the ethanol extract of the serum can be obtained.

[0017] During the extraction with ethanol, cold soaking, reflux, ultrasonic or microwave extraction can also be used.

Embodiment 2

[0018] Example 2 See the preparation of each effective fraction of serum

[0019] Take the ethanol extract of the serum prepared in Example 1, add water to suspend it, then extract with equal volumes of petroleum ether, diethyl ether, ethyl acetate, and n-butanol successively, extract each solvent 3 times, and collect the petroleum ether extract, Ether extract, ethyl acetate extract, n-butanol extract and raffinate, after recovering the solvent, dry under reduced pressure at 60°C to obtain the serum petroleum ether part, ethyl ether part, ethyl acetate part and n-butanol part respectively and the aqueous phase.

[0020] Will see the serum ether extract through repeated silica gel column chromatography, and gradient elution with different ratios of petroleum ether-ethyl acetate, petroleum ether: ethyl acetate (30:1) to obtain compound (1), petroleum ether: ethyl acetate (20:1) to obtain compound (2), petroleum ether: ethyl acetate (10:1) to obtain compound (3), and to apply a ...

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PUM

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Abstract

The invention discloses a nervate twayblade herb alcohol extract, a nervate twayblade herb ether extract, a nervate twayblade herb ethyl acetate, a nervate twayblade herb n-butyl alcohol extract, a nervate twayblade herb water phase extract and an extract comprising the ether extract, the ethyl acetate extract, the n-butyl alcohol extract and the water phase extract, and further discloses an application of the nervate twayblade herb extracts. The nervate twayblade herb extracts have good hemostatic activity, and can be widely used for treating in-vivo and in-vitro haemorrhage and operative hemorrhage.

Description

technical field [0001] The present invention relates to serum extract and its application. Background technique [0002] The serum is the whole herb of the orchid plant Liparis nervosa (Thunb.) Lindl., also known as "Lidihao", "Black Orchid", and "Short Plump". It is cold in nature, bitter in taste, cools blood to stop bleeding, Detoxification. See serum often grows in damp places such as mountains and streams, and is distributed in Sichuan, Hubei, Taiwan and other places. At present, the folk medicinal materials are all wild. [0003] So far, there are few domestic and foreign research reports on serum, and the literature records are not exhaustive enough. In the research on the hemostasis of Jianserum, Zhou Jiming et al. used Jianserum and other medicinal materials to formulate prescriptions, and found that the compound Jianserum hemostatic drug had a significant hemostatic effect on 54 cases of surgical incisions in 14 clinical categories, and the drugs could be absorbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/898A61P7/04
Inventor 宋芹杨伟峰苟小军陈封政颜军郭晓强
Owner CHENGDU UNIV
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