Preparation and hemostasis medical application of asiatic toddalia root extract containing rich furocoumarin

A technology of furanocoumarin and dragon's palm blood, applied in the field of natural medicinal chemistry, can solve the problems of uncertain clinical efficacy, lack of objective and reliable monitoring indicators, and incomplete disclosure of relationships, etc.

Inactive Publication Date: 2018-03-06
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Feilongzhang blood was first recorded in "Plant Mingshi Tukao". It is mainly distributed in Guizhou, Sichuan, Guangxi, Hunan and other places in my country. Also known as Jianxuefei, Xuejiansorrow, Jianxuesan, etc., but its pharmacological research has not fully revealed the relationship between the active ingredients of traditional Chinese medicine and the pharmacological action and mechanism of hemostasis, lack of objective and reliable monitoring indicators, and the clinical efficacy is uncertain

Method used

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  • Preparation and hemostasis medical application of asiatic toddalia root extract containing rich furocoumarin
  • Preparation and hemostasis medical application of asiatic toddalia root extract containing rich furocoumarin
  • Preparation and hemostasis medical application of asiatic toddalia root extract containing rich furocoumarin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation process of the Furocoumarin-rich Pterodactyl japonicus blood extract, the steps are as follows:

[0045] (1) Pulverize the dried medicinal material of Feilongzhangxue to obtain coarse powder;

[0046] (2) Cold soaking extraction, adding mass fraction to the dried medicinal material of Feilongzhang blood treated in step (1) is 70% ethanol aqueous solution, the weight ratio of dried medicinal material of Feilongzhangxue and ethanol is 1:6, each cold soaking extraction 3 days, a total of 6 times of cold-dipping extraction, the combined cold-dipping extract was concentrated into extract 1, and the dregs were set aside;

[0047] (3) Reflux extraction, the medicinal dregs processed through step (1) are reflux extracted with a mass fraction of 30% ethanol aqueous solution, the weight ratio of medicinal residues and ethanol aqueous solution is 1:3, each reflux extraction is 1.5h, and a total of 2 times, the combined reflux extract was concentrated to obtain ext...

Embodiment 2

[0053] The preparation process of the Furocoumarin-rich Pterodactyl japonicus blood extract, the steps are as follows:

[0054] (1) Pulverize the dried medicinal material of Feilongzhangxue and sieve to obtain fine powder;

[0055] (2) Cold soaking and extracting, adding mass fraction to the dried medicinal material of Feilongzhang blood treated in step (1) is 80% ethanol aqueous solution, the weight ratio of dried medicinal material of Feilongzhangxue and ethanol is 1:10, each cold soaking extraction 5 days, a total of 8 times of cold soaking and extraction, the combined cold soaking extract was concentrated into extract 1, and the dregs were set aside;

[0056] (3) Reflux extraction, the medicinal dregs processed through step (1) are reflux extracted with a mass fraction of 50% ethanol aqueous solution, the weight ratio of medicinal dregs and ethanol aqueous solution is 1:5, and each reflux extraction is 2h, and a total of 3 extractions are performed , combined and refluxed...

Embodiment 3

[0060] Example 3 Effects of different extraction methods on the hemostatic activity of Feilongzhangxue

[0061] 1.1 Experimental object: Kunming mice

[0062] 1.2 Investigation indicators: Determination of bleeding time, bleeding volume, and coagulation time of mice

[0063] 1.3 Before use, dissolve the impregnated extract in 0.5% CMC-Na to make three concentration solutions of 0.2g / mL, 0.1g / mL, 0.05g / mL, high, medium and low, and reflow the extract to make 0.1g / mL, 0.05 g / mL, 0.025g / mL, high, middle and low concentration solutions, the volume of intragastric administration is 10mL / kg.

[0064] 1.4 KM mice were randomly divided into groups, that is, the blank group (0.5g.kg -1 normal saline), Yunnan Baiyao group (0.5g·kg -1 ), the high, middle and low dose groups of Feilongzhangxue extract to be tested, 6 rats in each group. Oral administration, once a day, for 7 days in a row. 60 minutes after the last administration, the mice were cut transversely along the tip of the t...

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Abstract

The invention discloses preparation and hemostasis medical application of asiatic toddalia root extract containing rich furocoumarin. The preparation comprises the steps: smashing a dry medicinal material asiatic toddalia root; adding ethyl alcohol into the dry medicinal material asiatic toddalia root, quenching and extracting for multiple times, combining quenching and extracting solutions and concentrating into extractum (1); then utilizing ethyl alcohol reflux to extract decoction dregs for multiple times, combining reflux extracting solutions and concentrating to obtain extractum (2); sequentially extracting the extractum (1), the extractum (2) or combined extractum of the extractum (1) and the extractum (2) by an extracting solvent to obtain effective parts and treating the effectiveparts to obtain extract. As the obtained extract contains rich furoconmarin natural active compound, the extract can be applied to preparing novel hemostasis medicines.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to the preparation technology of the extract rich in furanocoumarin and the medical application of the extract in hemostasis. Background technique [0002] Hemorrhage generally refers to the phenomenon that blood flows out from the local damaged part after the human skin or tissue is injured. Generally, there is a hemostasis mechanism in the human body. Locally damaged blood vessels contract reflexively or platelets in damaged blood vessels aggregate to form clots, so that bleeding can be stopped by itself in the case of slow and small bleeding. But for the human body whose hemostatic mechanism is abnormal, it will cause a series of serious bleeding diseases, such as hemophilia. The application of hemostatic drugs to treat hemorrhagic diseases is the main means of clinical application at present. [0003] Feilongzhang blood was first recorded in "Plant Mingshi Tukao". It...

Claims

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Application Information

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IPC IPC(8): A61K36/75A61P7/04
CPCA61K36/75A61K2236/333A61K2236/39A61K2236/51
Inventor 周威郝小燕孙文博梁妍张晓燕
Owner GUIZHOU MEDICAL UNIV
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