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Serum-free medium for mesenchymal stem cells (MSCs) and preparation method of serum-free medium

A serum-free medium and stromal cell technology, applied in the field of serum-free medium for mesenchymal stem cells and their preparation, can solve the scientific research or clinical needs of mesenchymal stem cells, limit the application of serum-free medium, and slow the proliferation rate and other problems, to achieve the effect of helping cells maintain activity, avoid blood-borne contamination, and maintain a good growth state.

Active Publication Date: 2020-09-04
TIANXINHE SUZHOU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the use of serum-free medium for culturing mesenchymal stem cells is disclosed in the prior art, the current serum-free medium for mesenchymal stem cells has problems such as poor cell viability and slow proliferation rate, which cannot be used for scientific research on mesenchymal stem cells. Or clinical needs, which limit the application of serum-free medium in mesenchymal stem cell culture

Method used

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  • Serum-free medium for mesenchymal stem cells (MSCs) and preparation method of serum-free medium
  • Serum-free medium for mesenchymal stem cells (MSCs) and preparation method of serum-free medium
  • Serum-free medium for mesenchymal stem cells (MSCs) and preparation method of serum-free medium

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A serum-free medium for umbilical cord mesenchymal stem cells, including basal medium DMEM / F12, based on the final concentration of the medium, also includes the following components: recombinant human insulin 7.0mg / L, cholesterol 3.0mg / L, recombinant fibronectin 24.7ng / L, sodium linoleate 16.5μg / L, recombinant human transferrin 11.5mg / L, perilla seed protein 6.0mg / L, stilbene glycoside 9.0μg / L, white Veratrol 7.3μg / L, Ginkgo biloba extract 8.5μg / L.

[0028] The preparation method of the serum-free medium for umbilical cord mesenchymal stem cells comprises the following steps: adding recombinant human insulin, cholesterol, recombinant fibronectin, sodium linoleate, recombinant human transferrin, and perilla seed protein to the basal medium , stilbene glycosides, resveratrol, and ginkgo biloba extract, mixed evenly, filtered with a filter membrane with a pore size of 0.22 μm, and sterilized to obtain a serum-free medium for umbilical cord mesenchymal stem cells.

Embodiment 2

[0030] A serum-free medium for umbilical cord mesenchymal stem cells, including basal medium DMEM / F12, based on the final concentration of the medium, also includes the following components: recombinant human insulin 6.5mg / L, cholesterol 1.5mg / L, recombinant fibronectin 22.3μg / L, sodium linoleate 15.4μg / L, recombinant human transferrin 8.3mg / L, perilla seed protein 5.0mg / L, stilbene glycoside 8.5μg / L, white Veratrol 5.8μg / L, Ginkgo biloba extract 7.3μg / L.

[0031] The preparation method of the above serum-free medium for umbilical cord mesenchymal stem cells is the same as in Example 1.

Embodiment 3

[0033] A serum-free medium for umbilical cord mesenchymal stem cells, including basal medium DMEM / F12, based on the final concentration of the medium, also includes the following components: recombinant human insulin 9.0mg / L, cholesterol 4.0mg / L, recombinant fibronectin 25.0ng / L, sodium linoleate 17.2μg / L, recombinant human transferrin 12.4mg / L, perilla seed protein 8.0mg / L, stilbene glycoside 9.5μg / L, white Veratrol 8.4μg / L, Ginkgo biloba extract 9.0μg / L.

[0034] The preparation method of the above serum-free medium for umbilical cord mesenchymal stem cells is the same as in Example 1.

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Abstract

The invention discloses a serum-free medium for mesenchymal stem cells (MSCs). The serum-free medium comprises a basic medium, recombinant human insulin, cholesterol, recombinant fibronectin, sodium linoleate, recombinant human transferrin, perilla seed protein, stilbene glycoside, resveratrol and a ginkgo biloba extract. According to the serum-free medium, the recombinant human transferrin and the plant protein perilla seed protein are added to be compounded, the stilbene glycoside, the resveratrol and the ginkgo biloba extract are compounded for use, on one hand, the serum-free medium has effects of resisting oxidation and scavenging free radicals and contributes to keeping cell activity; and on the other hand, the serum-free medium still keeps a higher proliferation speed and a better growth state of cells under the condition that exogenous serum is not added. The serum-free medium comprises clear components, the repeatability, accuracy and stability of an experiment can be improved, raw materials are easy to obtain, and the preparation cost of the serum-free medium is effectively reduced. The invention further provides a preparation method of the serum-free medium, and the preparation process is convenient and easy to operate.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a serum-free medium for mesenchymal stem cells and a preparation method thereof. Background technique [0002] Human mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are a kind of adult stem cells with obvious plasticity and multi-directional differentiation potential, present in bone marrow, fat, synovium, bone, muscle, lung, liver, pancreas and other tissues as well as amniotic fluid, umbilical cord, etc. in the blood. Under the regulation of different growth factors, it can differentiate into different tissue cell types derived from the 3 germ layers, such as adipocytes, osteoblasts, hepatic-like cells, cardiomyocyte-like cells, glial cells, neuronal cells and islet-like cells Wait. In addition, human mesenchymal stem cells do not express major histocompatibility II antigens, but express a small amount of major histocompatibility class I antigens, have low immunogenicity, lo...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N5/0668C12N2500/25C12N2500/36C12N2500/76C12N2500/90C12N2501/998C12N2501/999
Inventor 张晓盈王小奇苏琳琳黄睿龙孙向东卢摇
Owner TIANXINHE SUZHOU BIOTECH CO LTD
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