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Fluorescent cellulase platinum nano-cluster and preparation method and application thereof

A cellulase, platinum nanotechnology, applied in nanotechnology, nanotechnology, fluorescence/phosphorescence, etc., can solve the problems of expensive instruments, high detection limit, poor specificity, etc. Effect

Active Publication Date: 2020-08-28
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the UV spectrophotometry, fluorescence method, high performance liquid chromatography-ultraviolet detection method and other methods used for its concentration detection have defects such as high detection limit, poor specificity, and expensive instruments.

Method used

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  • Fluorescent cellulase platinum nano-cluster and preparation method and application thereof
  • Fluorescent cellulase platinum nano-cluster and preparation method and application thereof
  • Fluorescent cellulase platinum nano-cluster and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of cellulase platinum nanoclusters, the specific steps are as follows:

[0039] (1) Take cellulase and add it to pure water to prepare a 1mM cellulase solution;

[0040] (2) Preparation of H at a concentration of 25 mM 2 PtCl 6 the aqueous solution;

[0041] (3) Put H 2 PtCl 6 The solution is added to the cellulase solution, so that the cellulase in it is mixed with H 2 PtCl 6 The molar ratio of the two pure raw materials is 3.5:1, and the vortexer is fully mixed, and the mixing time is 5min;

[0042] (4) then add the aqueous solution of 1M concentration NaOH in the solution obtained in step (3) to adjust the pH of the solution to be 12, mix quickly with a vortexer, and the mixing time is 5min;

[0043] (5) Under the condition of avoiding light, the prepared sample was placed in a water bath, the temperature was set to 37°C, and the reaction time was 12h to obtain a polymer of platinum nanoclusters. After centrifugation at 5,000 rpm / min fo...

Embodiment 2

[0048] Embodiment 2: Preparation of cellulase platinum nanoclusters, the specific steps are as follows:

[0049] (1) Add cellulase to pure water to prepare a 0.5mM cellulase solution;

[0050] (2) Prepare H at a concentration of 100 mM 2 PtCl 6 aqueous solution;

[0051] (3) H 2 PtCl 6 solution was added to the cellulase solution, cellulase and H 2 PtCl 6 The molar ratio is 0.5:1, and the vortex is fully mixed, and the mixing time is 5min;

[0052] (4) Then add 1M NaOH solution in the solution obtained in step (3) to adjust the pH to 12, fully mix with a vortex, and the mixing time is 5 minutes;

[0053] (5) Under the condition of avoiding light, put the prepared sample in a water bath, set the temperature at 37°C, and the reaction time is 12h to obtain the polymer of platinum nanoclusters, centrifuge at 5,000rpm / min for 10min, and take the supernatant solution and stored in a refrigerator at 4°C in the dark.

Embodiment 3

[0054] Embodiment 3: Preparation of cellulase platinum nanoclusters, the specific steps are as follows:

[0055] (1) Add cellulase to pure water to prepare a 2mM cellulase solution;

[0056] (2) 50mM H 2 PtCl 6 solution was added to the cellulase solution, cellulase and H 2 PtCl 6 The molar ratio is 3:1, and the vortex is fully mixed, and the mixing time is 5min;

[0057] (3) Then add 1M NaOH solution in the solution obtained in step (2) to adjust the pH to 12, and mix quickly with a vortex for 5 minutes;

[0058] (4) Under the condition of avoiding light, put the prepared sample in a water bath, set the temperature at 60°C, and the reaction time is 8h to obtain the polymer of platinum nanoclusters, centrifuge at 5,000rpm / min for 10min, and take the supernatant solution and stored in a refrigerator at 4°C in the dark.

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Abstract

The invention provides a green, simple and efficient fluorescent platinum nano-cluster and a preparation method and application thereof. The preparation method comprises the following steps: mixing acellulase solution with an H2PtCl6 solution, and sufficiently and uniformly mixing for 5-10 minutes by using a vortex device; adjusting the pH value of the NaOH solution to be 8-13, and uniformly mixing for 5-10 minutes again by using the vortex device; and reacting the prepared sample in a water bath kettle in a dark place for 6-24 hours. The nano-cluster shows blue fluorescence under the irradiation of a 305nm ultraviolet lamp, the maximum excitation wavelength of the nano-cluster is 350nm, and the maximum emission wavelength of the nano-cluster is 440nm. A detection method for detecting theampicillin concentration in an actual sample by using the fluorescent cellulase platinum nano-cluster is also established, and the method is stable, rapid, sensitive, strong in specificity and good in reproducibility, and can be used for monitoring the ampicillin content in the fields of environment, food, medicine, agricultural production and the like.

Description

technical field [0001] The invention relates to the field of preparation and application of functional fluorescent nanomaterials, and more particularly, to a fluorescent cellulase platinum nanocluster and a preparation method and application thereof. Background technique [0002] Cellulase is mainly derived from Aspergillus niger and Trichoderma. It is a general term for a group of enzymes that hydrolyze cellulose to generate glucose and low-polymerization fibers, including endoglucanase, exoglucanase and fiber two. Carbohydrase has 3 main components. At present, cellulase has been widely used in textiles, daily chemicals, papermaking, food fermentation, industrial washing, tobacco, oil extraction, waste water treatment and feed and other fields, and its application prospects are very broad. [0003] Metal nanoclusters have excellent optical stability, large Stokes shift and low toxicity, and can be used in analytical and detection fields such as metal ions, small biologica...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/87G01N21/64B82Y30/00B82Y40/00
CPCC09K11/025C09K11/87G01N21/6428B82Y30/00B82Y40/00G01N2021/6432
Inventor 王宝娟常欣吉成均高鹏王源秀吴爽陈杰吴雨芬魏晓丽
Owner ANHUI NORMAL UNIV
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