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Fluorescent gold nano-cluster taking cellulase as template and preparation method and application of fluorescent gold nano-cluster

A technology of cellulase and nanoclusters, applied in biochemical equipment and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problems of complex process, cost of money, long time, etc., and achieve simple operation , low cost, enhanced vitality effect

Active Publication Date: 2020-02-18
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current methods for detecting ascorbic acid, such as high performance liquid chromatography, flow injection chemiluminescence analysis, etc., usually require complex and precise instruments, and at the same time have the disadvantages of long time, complicated process, and cost of money.

Method used

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  • Fluorescent gold nano-cluster taking cellulase as template and preparation method and application of fluorescent gold nano-cluster
  • Fluorescent gold nano-cluster taking cellulase as template and preparation method and application of fluorescent gold nano-cluster
  • Fluorescent gold nano-cluster taking cellulase as template and preparation method and application of fluorescent gold nano-cluster

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: Preparation of cellulase gold nanoclusters, the specific steps are as follows:

[0053] (1) Add cellulase to pure water to prepare a 1 mM cellulase solution;

[0054] (2) Take 1M HAuCl 4 The solution was diluted with water to a concentration of 25mM HAuCl 4 solution;

[0055] (3) Add 16 μL of HAuCl 4 Add the solution to 984 μL cellulase solution to make the cellulase and HAuCl 4 The molar ratio is 2.5:1, and the vortex is fully mixed, and the mixing time is 5min;

[0056] (4) Then add 1M NaOH to the solution obtained in step (3) to adjust the pH to 12, and vortex to mix quickly, and the mixing time is 5min;

[0057] (5) Under the condition of avoiding light, put the prepared sample in a water bath, set the temperature at 37°C, and the reaction time is 12h to obtain the polymer of gold nanoclusters, centrifuge at 5,000rpm / min for 10min, and take the supernatant solution and stored in a refrigerator at 4°C in the dark.

[0058] Take the cellulase gold...

Embodiment 2

[0063] Embodiment 2: Preparation of cellulase gold nanoclusters, the specific steps are as follows:

[0064] (1) Add cellulase to pure water to prepare a 0.5mM cellulase solution;

[0065] (2) Take 1M HAuCl 4 The solution was diluted with water to a concentration of 100mM HAuCl 4 solution;

[0066] (3) HAuCl 4 solution was added to the cellulase solution, cellulase and HAuCl 4 The molar ratio is 0.5:1, and the vortex is fully mixed, and the mixing time is 5min;

[0067] (4) Then add 1M NaOH to the solution obtained in step (3) to adjust the pH to 12, and vortex to mix quickly, and the mixing time is 5min;

[0068] (5) Under the condition of avoiding light, put the prepared sample in a water bath, set the temperature at 37°C, and the reaction time is 12h to obtain the polymer of gold nanoclusters, centrifuge at 5,000rpm / min for 10min, and take the supernatant solution and stored in a refrigerator at 4°C in the dark.

Embodiment 3

[0069] Embodiment 3: Preparation of cellulase gold nanoclusters, the specific steps are as follows:

[0070] (1) Add cellulase to pure water to prepare a 2mM cellulase solution;

[0071] (2) Take 1M HAuCl 4 The solution was diluted with water to a concentration of 50mM HAuCl 4 solution;

[0072] (3) HAuCl 4 solution was added to the cellulase solution, cellulase and HAuCl 4 The molar ratio is 3:1, and the vortex is fully mixed, and the mixing time is 5min;

[0073] (4) Then add 1M NaOH to the solution obtained in step (3) to adjust the pH to 12, and vortex to mix quickly, and the mixing time is 5min;

[0074] (5) Under the condition of avoiding light, put the prepared sample in a water bath, set the temperature at 60°C, and the reaction time is 8h to obtain the polymer of gold nanoclusters, centrifuge at 5,000rpm / min for 10min, and take the supernatant solution and stored in a refrigerator at 4°C in the dark.

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Abstract

The invention belongs to the technical field of preparation and application of functional nano materials, a gold nano-cluster synthesized by taking cellulase as a template is successfully prepared byadopting a one-pot method, the nano-cluster shows red fluorescence under the irradiation of a 365nm ultraviolet lamp, the maximum excitation wavelength is 560nm, and the maximum emission wavelength is680nm. The invention also provides a method for fluorescence quantitative detection of metal ions, papain and ascorbic acid by utilizing the cellulase gold nano-cluster. The method is simple and convenient to operate, short in time consumption and easy to realize. The cellulase-coated gold nano-cluster prepared by the invention not only enhances the activity of the cellulase, but also can be applied to the aspects of environmental monitoring, food and medicine, agricultural production and the like on the basis of the capability of tracking biomolecules by strong red fluorescence.

Description

technical field [0001] The invention belongs to the technical field of preparation of fluorescent gold nanoclusters, in particular to the preparation of gold nanoclusters using cellulase as a template and its application in the detection of metal ions, enzymes and ascorbic acid. Background technique [0002] Cellulase is a type of glycoside hydrolase that can catalyze the hydrolysis of cellulose to generate glucose, also known as cellulase. Cellulase has been widely used in biomass ethanol, food, textile, feed and other industries due to its good biocompatibility, non-toxicity, diverse biological activities, high enzyme catalytic efficiency, and mild catalytic reaction conditions. [0003] Metal nanoclusters (NCs), usually composed of a few to tens of metal atoms, are a new class of fluorescent imaging materials. Metal nanoclusters can produce specific energy level separation and emit fluorescence under a certain wavelength of light excitation. Due to their unique electroni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58C09K11/02B82Y30/00C12Q1/34C12Q1/37G01N21/64
CPCC09K11/58C09K11/02G01N21/6428C12Q1/34C12Q1/37B82Y30/00
Inventor 王宝娟常欣吴雨芬吴爽李全发高鹏
Owner ANHUI NORMAL UNIV
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