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Electrochemical luminescence detection kit for zinc transporter 8 islet autoantibody

A reagent kit and electrochemical technology, applied in the field of biomedical detection, can solve problems such as limitations of clinical promotion work, affecting the specificity of test results, non-specific fluorescence interference, etc., and achieve the goals of avoiding data differences, high detection sensitivity, and avoiding high background Effect

Pending Publication Date: 2020-08-25
JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

However, due to the use of radionuclides, the RBA antibody detection method has brought limitations to clinical promotion.
The detection sensitivity of CLIA and LIPS methods is close to that of RBA method, but there may be problems such as non-specific fluorescence interference, which affects the specificity of the detection results

Method used

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  • Electrochemical luminescence detection kit for zinc transporter 8 islet autoantibody
  • Electrochemical luminescence detection kit for zinc transporter 8 islet autoantibody
  • Electrochemical luminescence detection kit for zinc transporter 8 islet autoantibody

Examples

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Embodiment 1

[0045] In this example, the main step of the RBA-ZnT8A detection is the rapid transcription and translation in the test tube. 35 After the S-labeled ZnT8 antigen protein is incubated with the serum to be tested overnight at 4°C, the antigen-antibody complex is captured by a 96-well polyvinylidene difluoride (PVDF) filter plate coated with protein A-Sepharose. After washing, the CPM (counts per minute, pulses per minute) value is obtained by using liquid scintillation fluid in a β-Counter counter. ZnT8A index = (specimen radiation count-negative ginseng radiation count) / (yang ginseng radiation count-negative See radiation count). Positive judgment standard: RBA-ZnT8A index ≥ 0.054.

[0046] Research objects include:

[0047] 1. Healthy control group: a total of 360 cases, no family history of diabetes and autoimmune disease, normal blood sugar on an empty stomach and 2 hours after a meal. From healthy volunteers and healthy people from Jiangsu Provincial People's Hospital Health...

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Abstract

The invention discloses an electrochemical luminescence detection kit for a zinc transporter 8 islet autoantibody (ZnT8A) and belongs to the technical field of biomedical detection. The detection principle of the kit is as follows, labeled recombinant ZnT8 antigen protein molecules are placed in a non-radioactive liquid phase environment for combination, the ZnT8 autoantibody in the serum is fullycombined with the recombinant ZnT8 islet antigen protein marked by the thioruthenium derivative active ester in a liquid phase environment, meanwhile, the recombinant ZnT8 islet antigen protein marked by the biotin polyethylene glycol active ester is grabbed and fixed on a streptavidin graphene flat plate, and the antibody amount in serum can be obtained by calculating a signal released by thio-ruthenium derivative active ester (Sulfo-TAG) combined with antigen protein of a specific antibody. The kit is advantaged in that the kit has relatively high sensitivity and specificity, is simple andconvenient to operate, has no radiation pollution, and has a very good clinical application value.

Description

Technical field [0001] The invention belongs to the technical field of biomedical detection, and specifically relates to an electrochemiluminescence detection kit for zinc transporter 8 pancreatic islet autoantibody and its application. Background technique [0002] Type 1 diabetes (type 1diabetes mellitus, T1DM) is an organ-specific autoimmunity that is triggered by certain environmental factors (viral infection, nutrition, etc.) in genetically susceptible individuals, and is characterized by islet β-cell damage. Sexual disease. Pancreatic islet autoantibodies are currently the most reliable biomarker of autoimmune destruction of pancreatic β-cells in T1DM. The detection of islet autoantibodies is helpful to understand the autoimmune course of T1DM, as well as the diagnosis and differential diagnosis of T1DM patients, as well as the general population and patients. The risk prediction of first-degree relatives is of great value. Currently recognized pancreatic islet autoantibo...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N21/76
CPCG01N33/6893G01N33/58G01N21/763G01N2800/042
Inventor 顾愹陈恒余金波徐宽枫杨涛
Owner JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV
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