Construction method of mouse animal model for simulating fracture intramedullary nail fixing system

Abstract

The invention discloses a construction method of a mouse animal model for simulating a fracture intramedullary nail fixing system. The construction method mainly comprises the steps that left hair andright hair are removed from the outer sides of thighs of a mouse in the longitudinal axis direction of the thighs; cutting open the skin along the longitudinal axis of the femur, bluntly separating the muscle gap between the lateral rectus femoris muscle and the hemitendinis muscle by using small hemostatic forceps, and exposing the femur; clamping the thighbone through tooth forceps, and pullingthe incision towards the far end of the thighbone; clamping a metal bar by a needle with the tip facing outwards, pulling the patella outwards by the tip at an exposed knee joint position, and then inserting the patella into the distal femur intercondylar fossa; rotating a needle holder, and inserting the metal bar from the distal femur fossa intercondylar fossa along the longitudinal axis of thefemur; pulling the incision back to the original lateral position of the femur, clamping a tooth tweezers clamp at one third of the proximal end of the femur, and adopting ophthalmic scissors for clamping the position of two fifths of the distal end of the femur and applying force. The problems that existing osteogenesis and fracture related animal model construction procedures are complex, experimental animal cost is high, and experimental data accuracy is not high are solved.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing a mouse animal model simulating a fracture intramedullary nail fixation system. Background technique [0002] In the current research field of osteogenesis and fracture-related medicine, small animals such as rats and mice mostly use bone defect animal models. For example, the patent application number 200510010795.5 discloses a method for establishing an animal model of traumatic limb deep vein thrombosis in rats. The orthopedic intramedullary nailing device is used to modify the self-made quantifiable hitting device to simulate the trauma process similar to orthopedic clinics. , causing different degrees of damage to the proximal ends of the lower limbs of rats, and creating an animal model of deep vein thrombosis with orthopedic clinical characteristics. The rats were hit on the proximal outer side of the bilateral thighs with diffe...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for constructing a mouse animal model simulating a fracture intramedullary nail fixation system, so as to solve the problem that the existing osteogenesis and fracture-related animal model construction procedures are relatively complicated, the cost of experimental animals is high, and the accuracy of experimental data is relatively high. not high problem

[0007] In order to realize the above-mentioned method of constructing a mouse animal model simulating a fracture intramedullary nail fixation system and solve the problems of the existing osteogenesis and fracture-related animal model construction procedures are relatively complicated, the cost of experimental animals is high, and the accuracy of experimental data is not high. The invention provides the following technical solutions:

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