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Method for simultaneously detecting acetamiprid and malathion

A technology for malathion and acetamiprid, which is applied in the field of simultaneous detection of acetamiprid and malathion, can solve the problems of time-consuming and high detection costs, and achieves improved stability, efficient immobilization, and good binding ability. Effect

Inactive Publication Date: 2020-08-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the field of pesticide residue analysis and detection, most of them involve the mixing of multiple pesticides. At present, the common electrochemiluminescence sensors can only detect one pesticide. When detecting multiple pesticides, it is necessary to prepare multiple sensors, which increases the detection cost and consumes a lot of time. plenty of time

Method used

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  • Method for simultaneously detecting acetamiprid and malathion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of hollow copper-cobalt-MOF:

[0031] (1) Take 1.83 g Co(NO 3 ) 2 The solid was mixed with 20 mL methanol solution (0.5 mol / L), and 1.88 g Cu(NO 3 ) 2 Solid with 20 mL of methanol solution (0.5 mol / L), take 1.3456 g of tetradecyltrimethylammonium bromide solid with 40 mL of aqueous solution (0.1 mol / L), take 1.7995 g of dimethylimidazole solid with 20 mL Methanol solution (1.096 mol / L);

[0032] (2) Add 375 μL 0.5 mol / L Co(NO 3 ) 2 methanol solution, 250 μL 0.5 mol / L Cu(NO 3 ) 2 Methanol solution and 10 mL of 0.1 mol / L tetradecyltrimethylammonium bromide aqueous solution; 63.75 mL of deionized water was added, and the mixture was magnetically stirred for 5 minutes, followed by the immediate addition of 6.5 mL of 1.096 mol / L dimethylimidazole Methanol solution; the reaction solution was continuously stirred for 90 minutes, and then the product was collected, washed with N-N-dimethylformamide and centrifuged twice, then washed with methanol a...

Embodiment 2

[0033] Example 2 g-C composited with gold nanoparticles 3 N 4 Preparation of:

[0034] (1) Weigh 10 g of urea into a crucible with an electronic balance, then put it into a tube furnace, heat up to 550 °C at a rate of 5 °C / min, and keep it warm for 4 h to obtain a light yellow powder C 3 N 4 ; Weigh the obtained C 3 N 4 100 mg in a conical flask, add 100 mL of ultrapure water, then sonicate for 16 h, the resulting solution is centrifuged at 3000 r / s to remove undispersed C 3 N 4 , collect the supernatant, and centrifuge at 10000r / s to obtain a precipitate, which is dissolved in 5 mL of ethanol. get a C 3 N 4 weak;

[0035] (2) Use a pipette to pipette 5 mL of C 3 N 4 Add 60 mL of AuNPs solution to the solution and test tube, put it on a magnetic stirrer and stir for 12 hours, centrifuge at 8500 r / s for 5 minutes in a centrifuge, and dissolve the obtained precipitate in 5 mL of ethanol solution to obtain g-C 3 N 4 -AuNPs solution.

Embodiment 3

[0036] Example 3 Preparation of complementary chain of acetamiprid aptamer loaded on hollow copper-cobalt-MOF:

[0037] (1) Take 1 mL of AuNPs in a beaker, add 20 mg of hydroxysuccinimide and 10 mg of carbodiimide, stir well, put it in an oven and incubate at 37 °C for 2 h, then add 1 mL of 0.01 mol / L The luminol solution was placed in an oven and incubated at 37 °C for 1 h to prepare the Luminol-AuNPs solution;

[0038] (2) Add 1 mL of Luminol-AuNPs to 1 mL of hollow copper-cobalt-MOF and stir on a magnetic stirrer for 12 h to prepare hollow copper-cobalt-MOF-Luminol-AuNPs;

[0039] (3) Use a pipette gun to pipette 20 μL of 0.1 μmol / L acetamiprid aptamer chain and slowly add it to 200 μL of hollow copper-cobalt-MOF-Luminol-AuNPs solution, shake it on the shaker for 30 minutes to shake homogeneously, and then placed in the refrigerator and incubated at 4 °C for 8 h to prepare hollow copper-cobalt-MOF-Luminol-AuNPs-cDNA1.

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Abstract

The invention relates to the technical field of pesticide residue analysis and determination, in particular to an electrochemiluminescence sensor constructed based on hollow copper-cobalt-MOF and usedfor simultaneously detecting two pesticides and a method for measuring acetamiprid and malathion by using the electrochemiluminescence sensor. The method comprises the steps of preparing the electrochemiluminescence sensor, operating the sensor to simultaneously measure acetamiprid and malathion and the like. According to the sensor for simultaneously detecting the two pesticides, hollow copper-cobalt-MOF enhanced luminol and g-C3N4 compounded by gold nanoparticles are used as an electrochemical luminescence substance, so that the two pesticides including acetamiprid and malathion are simultaneously detected. The electrochemiluminescence sensor for simultaneously detecting the two pesticides based on the hollow copper-cobalt-MOF is simple in material synthesis, small in interference of the two luminescent substances, high in sensitivity and good in selectivity.

Description

technical field [0001] The invention relates to the technical field of analysis and determination of pesticide residues, in particular to an electrochemiluminescence sensor based on hollow copper-cobalt-MOF for simultaneous detection of acetamiprid and malathion and the simultaneous determination of acetamiprid and malathion using it The method includes the preparation steps of the sensor for simultaneous detection of acetamiprid and malathion and the operation method of using the sensor to simultaneously determine acetamiprid and malathion; this electrochemiluminescent sensor can simultaneously detect two kinds of pesticides Perform qualitative and quantitative testing. Background technique [0002] Among the conventional analytical methods for pesticide residues, chromatography and its combined techniques still play an important role due to their high sensitivity and the ability to achieve multi-component determination. However, these methods generally have defects such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48G01N21/76B22F9/24B22F1/00C25D3/48C25D5/54B82Y15/00B82Y40/00
CPCG01N27/3278G01N27/308G01N27/48G01N21/76B22F9/24C25D3/48C25D5/54B82Y15/00B82Y40/00B22F1/054
Inventor 刘汉彪周长利李淑涵马东晓衣姜乐夏方诠田栋
Owner UNIV OF JINAN
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