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Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof

A technology for microbial fermentation bacteria and wheat straw, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that pathogenic bacteria are difficult to be completely removed, the activity of Fusarium graminearum cannot be inhibited, and the production efficiency of organic fertilizers is affected. and other problems, to achieve the effect of good field application effect, easy large-scale promotion, and shortened production cycle.

Inactive Publication Date: 2020-08-18
ANHUI SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there will be certain toxin residues in its meat, eggs, and milk products, which will cause diffuse harm to consumers.
[0004] What's more serious is that for the wheat infiltrated by Fusarium graminearum, after the wheat is mature and harvested, a large number of pathogenic bacteria remain in the wheat straw, and it is often difficult to completely remove the pathogenic bacteria in the process of composting and fermentation, thus limiting the quality of wheat. Application of organic fertilizer made from straw
[0005] In the process of straw fermentation, in order to speed up the rate of straw decomposing, fermenting agents are usually added to the fermented material. However, the straw fermenting agents widely used in the market are generally unable to inhibit the activity of Fusarium graminearum in the diseased wheat straw. Moreover, the fermentation time of the straw after adding the conventional fermentation agent still needs 15-30 days, which is still relatively long, which affects the overall production efficiency of organic fertilizer

Method used

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  • Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof
  • Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof
  • Microbial fermentation inoculant for accelerating wheat straw fermentation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment one: the expansion culture of microbial flora

[0039] 1. Straw raw material pretreatment: The infected straw was collected from the wheat field in Nanjing City, Jiangsu Province, crushed to powder, air-dried naturally, and set aside;

[0040] 2. Separation of microbial flora: put 100mL of sterile inorganic salt culture medium into a 250mL conical flask, add 5% of the straw into the conical flask according to the liquid volume, and culture on a shaker at 30°C at 180rpm for 48h. Transfer the bacterial flora suspension into a fresh 100mL sterile inorganic salt medium according to 10% of the liquid volume, continue to cultivate for 48 hours, and subculture 5 times according to the above method;

[0041] 3. Preparation of fermentation broth: put 400L of inorganic salt medium in a 500L fermenter, sterilize at 121°C, 0.1MPa for 30min, and cool to room temperature;

[0042] 4. Expansion of the flora: add the treated stalks into the fermenter in an amount of 5% of the...

Embodiment 2

[0044] Example 2. Determination of relevant parameters in the fermentation and composting of infected straw

[0045] 1. Preparation of Fusarium graminearum Spore Liquid

[0046] Transfer the activated Fusarium graminearum to the PDA plate, culture at 25°C for 5 days, pick the mycelia into the mung bean medium, culture at 25°C, 180rpm, on a shaker for 5 days, filter with gauze, and centrifuge the filtrate at 7000rpm for 10min. The pellet was resuspended in sterile water at a concentration of 10 5 cells / mL and stored in the refrigerator for later use.

[0047] 2. Fermentation of diseased wheat straw

[0048] 1) Experimental settings: three groups of treatments were set up, including a control group (natural fermentation of straw) and two experimental groups (using the microbial fermentation agent prepared by the present invention and Beijia organic fertilizer fermentation agent to treat the infected straw respectively), and compared The degree of fermented and decomposed stra...

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Abstract

The invention discloses a microbial fermentation inoculant for accelerating wheat straw fermentation and a preparation method of the microbial fermentation inoculant. The microbial agent is obtained by carrying out enlarged culture on microbial florae separated from infected straws, wherein the microbial flora mainly comprises two major components, namely bacteria and actinomycetes, and the identified bacterial components comprise bacillus, pseudomonas, pantoea, bifidobacterium, bacillus aceticus, lactobacillus, stenotrophomonas, klebsiella, citrobacter and flavobacterium. The microbial agentprepared by the invention has a very high prevention effect on wheat scab and gibberellic toxin, can play a role in inhibiting the growth of pathogenic bacteria in the fermentation process of wheat straw raw materials for preparing organic fertilizers, can also accelerate the fermentation and decomposition speed of straws, and is remarkable in effect and strong in universality.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculum, and in particular relates to a microbial fermentation inoculum for accelerating wheat straw fermentation and a preparation method thereof. Background technique [0002] Straw is rich in organic matter and a variety of nutrients needed for crop growth, and is an important raw material for fermentation into organic fertilizers. The principle of straw fermentation is that during the fermentation process, the organic matter in straw will undergo a complex transformation process under the action of microorganisms. First, the complex organic matter will be decomposed into simple substances, and finally carbon dioxide, water and mineral nutrients will be generated; followed by organic matter The resynthesis of organic humus is more complex, which is more conducive to the absorption and utilization of crops. Fermentation of straw to make organic fertilizer, on the one hand, can stop the burni...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/80C05F17/20C05F11/00C12R1/07C12R1/38C12R1/01C12R1/02C12R1/225C12R1/22C12R1/20
CPCC05F11/00C12N1/20C05F17/20C05F17/80Y02W30/40
Inventor 杨洪杏王翔马婷婷赵建荣张浩周椿富曹胜飞项进韩振鲍文梅程嘉蓉程嘉慧
Owner ANHUI SCI & TECH UNIV
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