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Application of microbial fermentation preparation in biological prevention and treatment on Empoasca pirisuga Matumura

A microbial fermentation agent, tea leafhopper technology, applied in the direction of chemicals for biological control, pest control, application, etc., can solve the problem of destroying ecological balance, to promote leaf development, reduce pesticide residues, and enhance photosynthesis The effect of action

Inactive Publication Date: 2020-08-14
江苏春江生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the defect that the chemical method in the prior art will destroy the ecological balance of tea leafhopper prevention and control, and to provide a kind of application of microbial fermentation preparation in biological control of tea leafhopper

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] It is obtained by inoculating the mixed strains of Bacillus highlander, Bacillus subtilis and Bacillus licheniformis according to the ratio of 2:4:1 and then carrying out liquid fermentation. The Bacillus uplander is the Bacillus sp. SYBC hb4 stored in the China Type Culture Collection Center of Wuhan University with the preservation number: CCTCC M2015018. During the fermentation, 50 g / L of betel nut, 10 g / L of oleander leaves and 35 g / L of teaku were added to the culture medium. Ferment with limited oxygen at 30°C for 6 days, then filter the fermented liquid under 100 mesh, dilute it 100 times, and spray it on the leaves. Observed in the field after 5 days, the degree of improvement of the tea leafhopper was more than 88%.

Embodiment 2

[0017] It is obtained by inoculating the mixed strains of Bacillus highlander, Bacillus subtilis and Bacillus licheniformis at a ratio of 1:3:1 and then carrying out liquid fermentation. The Bacillus uplander is the Bacillus sp. SYBC hb4 stored in the China Type Culture Collection Center of Wuhan University with the preservation number: CCTCC M2015018. During the fermentation, 45 g / L of betel nut, 8 g / L of oleander leaves and 45 g / L of tea dead were added in the culture medium. Ferment in limited oxygen at 32°C for 10 days, then filter the fermentation broth under 100 mesh, dilute it 80 times, and spray it on the leaves. Observed in the field after 5 days, the degree of improvement of the tea leafhopper was more than 80%.

Embodiment 3

[0019] It is obtained by inoculating the mixed strains of Bacillus highlander, Bacillus subtilis and Bacillus licheniformis according to the ratio of 2:5:1 and then carrying out liquid fermentation. The Bacillus uplander is the Bacillus sp. SYBC hb4 stored in the China Type Culture Collection Center of Wuhan University with the preservation number: CCTCC M2015018. During the fermentation, 65 g / L of betel nut, 15 g / L of oleander leaves and 45 g / L of tea dead were added in the culture medium. Ferment with limited oxygen at 28-32°C for 5 days, then filter the fermentation broth under 100 mesh, dilute it by 80-120 times and spray on the leaves. Observed in the field after 8 days, the degree of improvement of the tea leafhopper was more than 85%.

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Abstract

The invention discloses an application of a microbial fermentation preparation in biological prevention and treatment on Empoasca pirisuga Matumura. According to the invention, bacillus altitudinis, bacillus subtilis and bacillus licheniformis are fermented in a liquid fermentation broth added with betel nuts, oleander leaves and sasangua cakes, a large amount of plant secondary metabolites aimingat Empoasca pirisuga Matumura are generated in the fermentation liquid, so that the Empoasca pirisuga Matumura is favorably killed, meanwhile, surface active substances such as lipopeptide substancesand the like are generated, so that plant resistance is enhanced, non-specific resistance induced by secondary products is generated through induction, a large number of plant growth regulating substances are generated in fermentation liquor, normal physiological activities of plants are facilitated, inorganic nutrients of the plants are increased, and the tree vigor of the plants is enhanced. According to the method, Empoasca pirisuga Matumura can be prevented and treated, tea tree leaf development can be promoted, photosynthesis can be enhanced, leaf quality can be improved, the improvementdegree of tea scale insects after spraying can exceed 80% or above, meanwhile, pesticide residues can be reduced, and autumn tea picking safety can be improved.

Description

technical field [0001] The invention relates to the application of a microbial fermentation preparation in biological control of tea leafhoppers, belonging to the technical field of biological control. Background technique [0002] Tea green leafhopper, which belongs to the Hemiptera Leafhopper family, is the main pest of tea trees in my country. This insect mainly damages the buds and leaves of tea trees. The leaf margins of the damaged buds and leaves turn yellow, the veins turn red, and the leaf tips and margins shrivel and scorch, and the growth stagnates. , the buds and leaves fall off, seriously affecting the yield and quality of tea. [0003] For the chemical control of tea leafhoppers, 25% imidacloprid + 25% acetamiprid (2:1) can be used to dilute 1000-2500 times and spray to control the population density of small green leafhoppers. And the limitations of the area, etc., at the same time have a good control effect on the different insect states of the small green le...

Claims

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Application Information

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IPC IPC(8): A01N65/40A01N63/22A01P7/04A01P21/00
CPCA01N65/00A01N65/08A01N65/40A01N63/22
Inventor 刘莉戴陵江樊萍
Owner 江苏春江生物科技有限公司
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