Method of curing soil body through motivating and cultivating microorganism in situ

A technology for microbial and soil solidification, applied in soil protection, excavation, coastline protection, etc., can solve the problems of slow growth and reproduction of microorganisms, difficult to quantify the amount of solution, low survival rate, etc., achieve excellent solidification effect, reduce uneven distribution of microorganisms , the effect of high survival rate

Inactive Publication Date: 2020-08-07
NANJING UNIV +1
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Problems solved by technology

[0010] The purpose of the present invention is to overcome the defects such as slow growth and reproduction of microorganisms through external culture in the existing MICP solidified soil treatment, uneven distribution in the soil, low survival rate, and inapplicability to various actual engineering conditions, and provide a method for using The method of stimulating and cultivating in-situ microorganisms to solidify the soil, through the in-situ dosing of the stimulating liquid, promotes the mic

Method used

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  • Method of curing soil body through motivating and cultivating microorganism in situ
  • Method of curing soil body through motivating and cultivating microorganism in situ

Examples

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Embodiment 1

[0074] The soil in this example is a 45° slope, silty clay, and the soil permeability coefficient is 5×10 -6 cm / s, the project ambient temperature is 25°C, and the project needs to improve the erosion resistance of the soil and solve the problem of water and soil loss.

[0075] 1) The penetration strength of the soil is measured by the standard penetration test, and the erosion resistance of the soil is measured by the erosion test, where the erosion resistance of the soil is defined as the amount of soil produced per unit time.

[0076]2) Sampling is taken from the soil to be treated, and the porosity of the soil is determined to be 60%. Soil treatment area S is 10m 2 , the treatment depth H is 0.1m. By relation (1) V e = αnSH, calculate the single dose of excitation solution V e (m 3 ). Among them, the correction factor α of the amount of excitation liquid is taken as 1.05, and the single amount of excitation liquid is calculated as 0.63m 3 . By relation (2) V c =βV...

Embodiment 2

[0092] The soil conditions and engineering requirements of this embodiment are the same as in Embodiment 1, and the processing steps are basically the same as in Embodiment 1, the difference being:

[0093] The correction factor α for the dosage of the excitation solution is taken as 1.15; the ratio β of the single dosage of the cementing fluid to the single dosage of the stimulation solution is taken as 0.9. By relation (1) V e = αnSH calculates that the single dosage of excitation solution is 0.69m 3 , through the relationship (2) V c =βV e The single dosage of cementing liquid is calculated as 0.621m 3 .

[0094] In this example, after the 5th round of spraying the excitation liquid, stop adding liquid; after spraying the 6th round of cementing liquid, stop adding liquid.

[0095] Experimental results: In this example, this method was used to stimulate urease-producing microorganisms in situ, and after MICP treatment, the calcium carbonate content of the soil was 6.3%,...

Embodiment 3

[0097] The soil conditions and engineering requirements of this embodiment are the same as in Embodiment 1, and the processing steps are basically the same as in Embodiment 1, the difference being:

[0098] The correction factor α for the amount of excitation liquid is taken as 1.1; the ratio β of the single amount of cementing liquid to the single amount of excitation liquid is taken as 1.1. By relation (1) V e = αnSH calculates that the single dosage of excitation solution is 0.66m 3 , through the relationship (2) V c =βV e Calculate the single dosage of cementing liquid is 0.726m 3 .

[0099] The components of the excitation solution were 200mM urea, 20g / L yeast extract, 40g / L glucose and 0.13MTris-base buffer; the components of the cementing solution were 1.0M urea, 1.0M calcium chloride and 5g / L nutrient broth.

[0100] In this example, after the 5th round of spraying the excitation liquid, stop adding liquid; after spraying the 6th round of cementing liquid, stop ad...

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Abstract

The invention discloses a method of curing a soil body through motivating and cultivating microorganism in situ. The method comprises the following steps: at first, adding motivating liquid in the soil body to promote reproduction of microorganism in the soil body, then, adding cementing liquid in the soil body to cure the soil body, and further, calculating the usage amount of the motivating liquid and/or cementing liquid added each time through the porosity, the treatment area and the treatment depth of the soil body. Through adding the motivating liquid in situ, reproduction of self microorganism in the soil body is promoted; the in-situ motivated microorganism in the soil body environment is balanced in nutrient substances, has interactive adjustment and sensing with other microorganism, grows and breeds rapidly and is high in urease activity; and in addition, the in-situ microorganism motivating method reduces the process of adding bacteria solution in the soil body, greatly reduces the influence of non-uniform solution flowing to non-uniform microorganism distribution and effectively improves the MICP effect.

Description

technical field [0001] The invention belongs to the interdisciplinary field of geotechnical engineering and microbial engineering, and more specifically relates to a method for solidifying soil by stimulating and cultivating in-situ microorganisms. Background technique [0002] With the rapid development of the world economy and the rapid increase of the population, the demand for construction land continues to increase. Especially in our country, urbanization continues to intensify, and more infrastructure is needed to meet the needs of the growing population. However, in the process of construction, there is a serious problem of insufficient land supply, increasing land consumption and deteriorating soil quality, which greatly limit the construction process of urbanization. Therefore, improving the soil that cannot meet the needs of engineering construction and improving soil utilization has become a major issue related to the national economy and people's livelihood. Ho...

Claims

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Application Information

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IPC IPC(8): E02D3/12E02D17/20E02B3/12
CPCE02B3/12E02D3/12E02D17/20
Inventor 唐朝生程瑶佳谢约翰刘博吕超李昊王殿龙施斌
Owner NANJING UNIV
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