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Method for continuously culturing tribonema

A technology of yellow hair algae and culture medium, applied in the direction of sterilization method, microorganism-based method, support/immobilization method of microorganism, etc., can solve the problems of low cultivation efficiency and non-continuous cultivation of cultivation, and achieve the purpose of increasing biomass, Enhancement of algae cell activity, the effect of stabilizing and efficient industrial production of algae algae

Pending Publication Date: 2020-08-07
QINGDAO XUNON BIOLOGICAL ENG
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Problems solved by technology

[0004] In order to solve the problem of non-continuous cultivation and low cultivation efficiency in the prior art, the present invention provides a method for continuously cultivating Xanthophyllum, realizing stable and efficient continuous production of Xanthophyllum, through pure cultivation of algal species → two Expansion of high-grade algae species→Industrial cultivation of Xanthophyllum closed loop reactor, and finally realize the continuous industrial production of Xanthophyllum. The cultivation method of raw materials realizes the continuous cycle industrial production of yellow hair algae

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  • Method for continuously culturing tribonema
  • Method for continuously culturing tribonema

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Embodiment Construction

[0022] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0023] Please refer to the accompanying drawings. A method for continuously cultivating yellow hair algae, comprising the steps of,

[0024] Step 1, build a continuous culture reactor: the reactor includes a nutrient solution storage tank 1, a number of closed glass plates 3, a collection plate 5, a filter device 6, a circulation pump 7, a fan 9, and nutrient solution pipelines and ventilation pipelines , wherein the nutrient solution storage tank 1 can be supplemented with nutrient solution, and the closed glass plate 3 can be opened. The nutrient solution storage tank 1 is connected to one end of several closed glass plates 3 through nutrient solution pipelines, and the other ends of the closed glass plates 3 are respectively connected to the collection plate 5 through nutrient solution pipelines. The plate 5 is similar in structure to th...

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Abstract

A method for continuously culturing tribonema comprises the following steps: 1, building of a continuous culture reactor; 2, expanding culture of algae species; 3, expanding culture of algae species in the reactor; and 4, algae species harvesting and re-culturing. The method for continuously culturing tribonema realizes stable and efficient continuous production of the tribonema, and can finally realize the continuous industrial production of the tribonema through pure culture of algae species, secondary expanding culture of the algae species and industrial culture of the tribonema in the closed circulating reactor, the inoculation frequency of the tribonema in industrial expanding culture can be reduced, and the continuous circulating industrial production of the tribonema is realized through a fed-batch cultivation mode.

Description

technical field [0001] The invention belongs to the field of industrialized cultivation of microalgae, and relates to a method for continuously cultivating yellow algae. Background technique [0002] Tribonema (Tribonema.sp) is a kind of yellow-green algae whose plant body is unbranched filaments. It belongs to the zooxanthellae. Tribonema mostly grows in ponds and humid freshwater environments, and has strong environmental adaptability. In addition, Xanthophyllum has good industrial properties such as high oil yield (50-60% oil content), anti-pollution, and easy harvesting, and is an ideal material for biodiesel production. Xanthophyllum has good nitrogen and phosphorus absorption capacity and can be used in sewage treatment. In addition, the oil extracted from Xanthophyllum contains higher content of palmitoleic acid and EPA (docosapentaenoic acid). This fatty acid belongs to Omega-7 series polyunsaturated fatty acids, this fatty acid has very important effects on both aq...

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12M1/12C12R1/89
CPCC12N1/12C12M37/04C12M47/10
Inventor 王志臻孙发强王珊珊刘珊珊高凯
Owner QINGDAO XUNON BIOLOGICAL ENG
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