Antibacterial, bacteriostatic and halitosis-resistant toothpaste prepared by mixing natural extracts and vitamin C, and preparation method thereof
A technology of natural extracts and manufacturing methods, applied in the field of antibacterial, antibacterial, anti-halitosis toothpaste and manufacturing, can solve the problems of low stability, narrow antibacterial spectrum, pigment, etc., and achieve the effect of outstanding antiseptic effect and simple process
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[0041] The preparation of natural extract possesses and comprises the following steps:
[0042] (1) Quantify licorice, grape seed extract, scutellaria baicalensis, ginkgo leaf extract and grapefruit respectively, then wash and dry with purified water;
[0043] (2) Add 300-600ml of ethanol with a concentration of 60-80% to licorice, grape seed extract, scutellaria baicalensis, ginkgo leaf extract and grapefruit raw materials, and extract at 3-5°C for 2-4 days Active ingredients;
[0044] (3) The active ingredients of licorice, grape seed extract, scutellaria baicalensis, ginkgo leaf extract and grapefruit are filtered through a pressure reducer and fertilizer paper, and concentrated to a high-concentration extract using a rotary concentrator;
[0045] (4) After filtering the concentrated high-concentration extract with fertilizer paper, quantify it with distilled water;
[0046] (5) The respective extracts consisting of quantitative licorice, grape seed extract, scutellaria b...
Embodiment 1
[0063] Antiseptic activity test of natural extract (NP-5): take out 1ml of natural extract of the present invention and 9ml of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, human Candida albicans, each bacterial strain culture fluid of Aspergillus niger mix . The concentration of the strain culture solution added at this time was about 1.0×10 5 , the content of the extract is 10% of the whole volume. The mixed extracts were stored at 25° C., and 1 ml was put into solid medium after 3 days / 1 week / 2 weeks / 3 weeks, respectively. At this time, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were cultured on trypticase soybean agar medium, human Candida albicans, and Aspergillus niger on PDA solid medium. Cultivate Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa at 37°C for 2 days, cultivate human Candida albicans, and Aspergillus niger at 27°C for 3 days, and perform cell counting after the cultivation is completed. The...
Embodiment 2
[0070] Antiseptic activity test of vitamin C mixture: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, human Candida albicans, Aspergillus niger and vitamin C mixture were mixed. The strain concentration was 1.0×10 5 , the total volume is 10ml, because the mixing ratio of the vitamin C mixture is 2%, 5%, 7%, 10%, and 20%, so the respective concentrations are also different. Then take Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, human Candida albicans, Aspergillus niger and vitamin C mixture 1ml and put it on the solid medium, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa Agar medium, human Candida albicans and Aspergillus niger were cultured on PDA solid medium. Cultivate Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa at 37°C for 2 days, and cultivate human Candida albicans and Aspergillus niger at 27°C for 3 days. After the culture is completed, count the cells and measure the concentration of vitamin...
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