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Application of plant selenium peptide in preparation of composition for improving obesity and metabolic syndrome

A technology for metabolic syndrome and plant selenium is applied in the application field of plant selenopeptide in the preparation of compositions for improving obesity and metabolic syndrome, and can solve the problems of unclear mechanism of action, diabetes, side effects of diabetes and the like

Pending Publication Date: 2020-08-07
恩施德源硒材料工程科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it is reported that selenium may enhance the antioxidant effect or promote insulin secretion, but the mechanism of action is unclear. In addition, inorganic selenium exceeding a certain dose may have certain side effects on diabetes, and even cause diabetes.
There are no clear reports on the protective effects of phytoselenopeptides on obesity and metabolic syndrome and the improvement of oxidative stress

Method used

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  • Application of plant selenium peptide in preparation of composition for improving obesity and metabolic syndrome
  • Application of plant selenium peptide in preparation of composition for improving obesity and metabolic syndrome
  • Application of plant selenium peptide in preparation of composition for improving obesity and metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Preparation of organic selenium plant selenopeptide (CSP)

[0043] Viola leaf broken Michelina powder (protein 14.42%, total selenium 1421ppm) is immersed in NaOH solution (PH8.0) according to the ratio of substrate concentration 1:20, then with enzyme and protein material ratio 2:100 (g / g) Add alkaline protease (Wuxi Genencor Company) at 50°C for enzymolysis for 3 hours, then add neutral protease (Wuxi Genencor Company) at an enzyme-to-protein material ratio of 1:100 (g / g) to adjust the pH to 7.0, hydrolyzed at 50°C for 3 hours. Inactivate the enzyme in a boiling water bath for 10 minutes, then centrifuge at 4000 r / min for 10 minutes, recover the supernatant, further concentrate by ultrafiltration, and freeze-dry to obtain organic selenium phytoselenopeptides (hereinafter referred to as CSP).

[0044] After testing, the total selenium content of the plant selenopeptide obtained in this embodiment is 1177ppm, the organic selenium content exceeds 90%, and t...

Embodiment 2

[0046] Example 2 Establishment of obesity model, phytoselenopeptide (CSP) alleviates obesity in mice induced by high-fat diet

[0047] Forty SPF grade C57BL / 6J mice (male, 6 weeks old, weighing 19-21 g) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. All animal experiments involved in the research were approved by the Animal Welfare and Ethics Committee of Beijing Experimental Animal Center.

[0048] The mouse model was established as follows:

[0049] The experimental animals were subjected to a standard light-dark cycle of 12 hours, a temperature of 23±2°C, and a relative humidity of 55±5%, and were adaptively fed for one week (the mice were all given NC feed (basic feed, purchased from Nantong Trophy Feed Technology Co., Ltd.) company, TP23300) free access to sterile water). After adaptation, 40 C57BL / 6J mice were randomly divided into four groups, 10 mice in each group:

[0050] (1) NCD group (CK control group), continued to be fed wit...

Embodiment 3

[0058] Example 3 Phytoselenopeptide (CSP) alleviates hypertrophy of liver and adipose tissue induced by high-fat diet

[0059] The freshly separated liver tissue and epididymal white adipose tissue of the four groups of mice in Example 2 were fixed in 10% neutral formalin solution, then embedded in paraffin, cut into 5 μm tissue slices, and then HE staining. Another part of liver tissue was taken, cut into 6 μm tissue thin slices, and stained with oil red. Tissue sections were analyzed under a microscope (200x).

[0060] Microscopic examination results such as figure 2 As shown in A, HE staining showed normal size white adipose tissue in the normal group (NCD), while severe fat hypertrophy and a large number of single fat vesicles appeared in the high-fat diet group (HFD). However, CSP supplementation (CSPL, CSPH) restored high-fat diet-induced adipose tissue abnormalities, especially in the CSPH supplementation group (CSPH).

[0061] Similar results also appeared in oil ...

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Abstract

The invention discloses an application of a plant selenium peptide in preparation of a composition for improving obesity and metabolic syndrome. The composition comprises foods, health care products,medicines, nutritional supplements, food additives and beverages. The plant selenium peptide is preferably an organic selenium source extracted from cruciferous plants, the organic selenium content ishigher than 90%, and the peptide content is higher than 10%. An embodiment proves that the plant selenium peptide can improve oxidative stress, enhance the activity of antioxidant enzymes such as GPx, SOD, CAT and TAOC and reduce the MDA level. The expression levels of inflammatory factors IL-6, TNF-alpha, IL-1 beta and MCP-1 are inhibited, the expression of genes FABP4, FAS, PPAR-gamma, SREBP1,HMGR and ACAT is inhibited, and the expression of lipid decomposition genes PPAR-alpha, PGC1-alpha, LPL, GPX and LCAT is promoted, the integrity of intestinal mucosa is maintained, and the expressionlevels of tight junction proteins ZO-1 and ccludin are up-regulated, the composition of intestinal microorganisms is regulated and controlled, the production of beneficial bacteria, namely Blauia, Blauia and Lactobacillus, is promoted, and the preparation method has a wide application prospect.

Description

technical field [0001] This application relates to the application field of selenopeptides, in particular to the application of a plant selenopeptide in the preparation of compositions for improving obesity and metabolic syndrome. Background technique [0002] With the rapid development of economy and the change of people's lifestyle, obesity has become the most important public health problem in the world. According to a 2017 report by The Lancet, more than 2.2 billion people worldwide are overweight and 1.3 billion adults are obese. In addition, increasing evidence shows that obesity is a high-risk factor for many chronic diseases including type 2 diabetes, non-alcoholic fatty liver disease, cardiovascular disease and many cancers, which seriously affects the quality of life and increases the global medical burden. Therefore, it is of great practical significance to expand more effective methods to prevent obesity and its complications. [0003] The clinical features of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/16A23L33/18A61K38/01A61K36/31A61P3/04A61P3/06A61P3/10A61P3/00
CPCA23L33/16A23L33/18A61K38/011A61K36/31A61P3/04A61P3/06A61P3/10A61P3/00A23V2002/00A23V2200/332A23V2200/3262A23V2200/328A23V2250/1626A23V2250/55A23V2200/30A23V2200/32
Inventor 于添丛欣刘海远
Owner 恩施德源硒材料工程科技有限公司
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