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SNP locus related to birth weight of sheep, application, molecular marker and primer

A technology of molecular markers and birth weight, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of undiscovered patent documents and achieve the goal of shortening the breeding cycle and improving reliability Effect

Active Publication Date: 2020-08-04
天津奥群牧业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through searching, no published patent documents related to this patent application have been found

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  • SNP locus related to birth weight of sheep, application, molecular marker and primer
  • SNP locus related to birth weight of sheep, application, molecular marker and primer
  • SNP locus related to birth weight of sheep, application, molecular marker and primer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] 1. Source of experimental samples

[0061] The experimental samples came from Australian white sheep locally bred by Tianjin Aoqun Animal Husbandry Co., Ltd., a total of 2633. Among them, 2603 underwent simplified genome sequencing, and 30 underwent whole genome resequencing.

[0062] 2. Birth weight record

[0063] The sheep's weight information record is accompanied by the whole production cycle of the sheep, and the record includes ear number, measurement date, date of birth, sex, breed, weighing type (whether birth weight), weight and other information. In the present invention, birth weight information is extracted for subsequent analysis. Take the first 3 records after sorting as an example, as shown in Table 1.

[0064] Table 1: Example birth weight information for Australian white sheep

[0065] number ID gender date of birth Birth weight (kg) AW2127 F 2014 / 1 / 15 3.5 AW2126 F 2014 / 1 / 23 3.0 AW0779 M 2015 / 1 / 25 3.4

...

Embodiment 2

[0079] This example is the effect of the SNP site chr8_81799821_G / A in Example 1 on the birth weight of different genotypes in 2496 sheep individuals. The R software aov() function was used to conduct multivariate analysis of variance to analyze the effect of different genotypes in the chr8_81799821_G / A nucleotide site on the birth weight of sheep. The analysis model is as follows:

[0080] P ijn =Y i +S j +G n +e ijn

[0081] Among them, P ijn Birth weight of sheep, Y i is the i-th year effect, S j is the nth seasonal effect, G n is the nth genotype effect, e ijn is a random residual.

[0082] It can be seen from Table 2 that in 2496 sheep populations, the birth weight of GG individuals in the chr8_81799821_G / A locus was significantly higher than that of AA individuals (P<0.01), and the birth weight of GA individuals was significantly higher than that of AA individuals. Birth weight (P<0.01)

[0083] Table 2: Effects of different genotypes in the chr8_81799821_G / ...

Embodiment 3

[0087] This example is the design and amplification results of upstream and downstream primers for the SNP site chr8_81799821_G / A obtained in Example 1.

[0088] 1. DNA extraction

[0089] According to the results of whole-genome DNA sequencing, a number of sheep ear samples with chr8_81799821_G / A nucleotide sites of GG, GA and AA genotypes were respectively selected, and the genomic DNA of the ear tissue was extracted by the method in Example 1. After quality and concentration detection Store at -20°C for later use.

[0090] 2. PCR amplification and sequencing

[0091] Using the extracted DNA as a template, carry out PCR amplification according to the designed primers: take 2.5 μL of DNA template, 1.25 μL of each primer shown in SEQ No.2 and SEQ No.3, 25 μL of TAKARA rTaq PCR Mix, double distilled water 20 μL; set the PCR amplification program: pre-denaturation at 98°C, 2min; denaturation at 98°C, 15s; annealing at 56°C, 30s; extension at 72°C, 20s; 35 cycles; extension at ...

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Abstract

The invention relates to an SNP locus related to birth weight of sheep. The locus is positioned at the 8th chromosome and the 81799821st chromosome of a sheep genome, the original basic group is G, and the mutation form is A. According to the method, a simplified genome sequencing means and sheep birth weight information are utilized, and the marker related to the sheep birth weight is positionedby combining whole genome association analysis (GWAS), so that the method has important guiding significance for increasing the birth weight of sheep and improving the production performance of sheepflocks, and provides a scientific basis for sustainable development of the sheep breeding industry.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding, in particular to a SNP site related to sheep birth weight, application, molecular marker and primer. Background technique [0002] With the adjustment of the agricultural industry and the changes in the dietary structure of residents, the status of the mutton sheep industry in the consumption of agricultural products in my country has gradually increased. The development of the sheep industry is accompanied by the demand for breed breeding and improvement. How to efficiently improve the breeding progress has always been the focus of breeders. [0003] Birth weight of sheep is an important part of modern sheep breeding, and it is the earliest available growth trait, which is positively correlated with early survival rate, weaning weight, growth speed and slaughter weight of lambs. Strengthening the genetic improvement of birth weight can effectively improve the production performance o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 张清峰沈俊燃王然杨漫漫林春建卢小芳
Owner 天津奥群牧业有限公司
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