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Nano material for osteoclast acidic closed region and preparation method thereof

A technology of nanomaterials and osteoclasts, applied in medical preparations with non-active ingredients, medical preparations containing active ingredients, bone diseases, etc., to improve drug utilization, improve trabecular bone gap, and reduce side effects

Pending Publication Date: 2020-08-04
THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the previous materials are still difficult to solve two problems at the same time, that is, the precision of osteoclast targeting and the physiology of the acting drugs.

Method used

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  • Nano material for osteoclast acidic closed region and preparation method thereof
  • Nano material for osteoclast acidic closed region and preparation method thereof
  • Nano material for osteoclast acidic closed region and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, preparation of tetracycline-modified nanoliposomes loaded with sodium bicarbonate

[0033] 1. Dissolve 20.00 mg of DSPE-PEG-NHS and 3.05 mg of tetracycline in 10.00 mL of chloroform, add triethylamine to adjust the pH to 8.2, and cross-link with magnetic stirring at room temperature for 48 hours.

[0034] 2. Dissolve the product obtained in step 1, 100.00 mg lecithin, and 16.00 mg cholesterol in chloroform, and make a thin film in a rotary evaporator.

[0035] 3. Add 10mL of 1mol / L sodium bicarbonate solution into the flask for hydration by shaking.

[0036] 4. Perform phacoemulsification. The phacoemulsification process is 2s on, 3s off, 40% power, and 10 minutes

[0037] 5. Dialyze in the dialysis bag for 72 hours, take it out and filter it through a 0.22 micron filter head, and store it at 4 degrees.

Embodiment 2

[0038] Example 2, preparation of alendronic acid-modified nanoliposomes loaded with sodium bicarbonate

[0039] 1. Dissolve 20.00 mg of DSPE-PEG-NHS and 2.30 mg of alendronate sodium in 10.00 mL of chloroform, add triethylamine to adjust the pH to 8.2, and cross-link with magnetic stirring at room temperature for 48 hours.

[0040] 2. Dissolve the product obtained in step 1, 100.00 mg lecithin, and 16.00 mg cholesterol in chloroform, and make a thin film in a rotary evaporator.

[0041] 3. Add 10mL of 1mol / L sodium bicarbonate solution into the flask for hydration by shaking.

[0042] 4. Perform phacoemulsification. The phacoemulsification process is 2s on, 3s off, 40% power, and 20 minutes

[0043] 5. Dialyze in the dialysis bag for 72 hours, take it out and filter it through a 0.22 micron filter head, and store it at 4 degrees.

Embodiment 3

[0044] Embodiment 3, preparation of tetracycline-modified nanoliposomes loaded with potassium bicarbonate

[0045] 1. Dissolve 20.00 mg of DSPE-PEG-NHS and 3.05 mg of tetracycline in 10.00 mL of chloroform, add triethylamine to adjust the pH to 8.2, and cross-link with magnetic stirring at room temperature for 24 hours.

[0046] 2. Dissolve the product obtained in step 1, 80 mg lecithin, and 16.00 mg cholesterol in chloroform, and make a thin film in a rotary evaporator.

[0047] 3. Add 10mL of 1mol / L potassium bicarbonate solution to the flask for hydration by shaking.

[0048] 4. Perform phacoemulsification. The phacoemulsification process is 2s on, 3s off, 40% power, and 10 minutes

[0049] 5. Dialyze in the dialysis bag for 72 hours, take it out and filter it through a 0.22 micron filter head, and store it at 4 degrees.

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Abstract

The invention discloses a nano material for an osteoclast acidic closed region and a preparation method thereof. The nano material for the osteoclast acidic closed region comprises a nano material, bone targeting molecules and a compound which generates a chemical reaction on the osteoclast acidic closed region, after the nano material is subjected to bone targeting molecular modification, a compound which generates a chemical reaction on an osteoclast acidic region is entrapped; according to the nano material, osteoclasts are inhibited through accurate mature osteoclast targeting and physiological chemical reaction regulated biological cascade reaction, and a new thought and a new tool are provided for pharmaceutical treatment of osteoclast abnormal activation.

Description

technical field [0001] The invention belongs to the field of bone tissue drug treatment, and in particular relates to a nanometer material for the acidic sealing area of ​​osteoclasts and a preparation method thereof. Background technique [0002] Osteoporosis is a global chronic disease that can lead to severe bone loss and fractures, causing suffering for patients and seriously reducing the quality of life. Abnormal activation of osteoclasts in bone tumors and bone metastasis of tumors can also cause abnormal activation of osteoclasts, resulting in osteoporosis and pathological fractures. At present, the treatment for abnormal activation of osteoclasts mainly includes calcium, vitamin D, calcitonin, bisphosphonates, estrogen and other bone resorption inhibitors and fluoride, synthetic steroids, parathyroid hormone and other bone formation promoting drugs. Although treatment methods can affect the function of osteoclasts or delay the course of the disease by promoting oste...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/54A61K33/00A61P19/10
CPCA61K47/6911A61K47/548A61K47/54A61K33/00A61P19/10
Inventor 林贤丰顾辰辉王清清范顺武
Owner THE AFFILIATED SIR RUN RUN SHAW HOSPITAL OF SCHOOL OF MEDICINE ZHEJIANG UNIV
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