Sesame polypeptide and preparation method and application thereof

A technology of sesame and solid-phase synthesis, which is applied in the field of deep processing of plant protein, can solve the problems of low processing degree of sesame protein, underutilization of sesame protein resources, backward processing technology, etc., and achieve cost controllable, significant activity, and raw material utilization high rate effect

Active Publication Date: 2020-07-31
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the main component of sesame—sesame protein (accounting for about 20% of the total weight of sesame), due to backward processing technology, the processing degree of sesame protein is relatively low, and it is mostly processed into feed or fertilizer, and the resource of sesame protein has not been fully utilized.

Method used

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  • Sesame polypeptide and preparation method and application thereof
  • Sesame polypeptide and preparation method and application thereof
  • Sesame polypeptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for extracting sesame polypeptide from sesame protein, which specifically comprises the steps:

[0035] 1) Sesame protein extraction:

[0036] Take 0.5 kg of sesame without impurities and mold particles, crush it to 60 mesh, and use a Soxhlet extractor for degreasing. h. Add the defatted sesame seeds to distilled water at a material-to-liquid ratio (w / v) of 1g:12ml, adjust the pH to 10 with sodium hydroxide, stir at room temperature for 90 minutes, then centrifuge at 4000 r / min for 30 minutes, take the supernatant and adjust the pH To 4.5, centrifuge at 4500 r / min for 30 min, collect the precipitate, wash with distilled water 3 times, freeze-dry to obtain sesame protein, and seal it for later use.

[0037] 2) Proteolysis:

[0038] The obtained sesame protein in step 1) is added in distilled water to obtain a sesame protein solution according to the material-to-liquid ratio of 1g:10ml (w / v), the pH value is adjusted to 2.0, and 0.5% (w / v) is added, that is, 0...

Embodiment 2

[0044] A method for artificially synthesizing sesame polypeptide, which adopts solid-phase synthesis method for artificial synthesis. The basic process is as follows: first, an amino acid whose amino group is protected by the Fmoc group is attached to the insoluble solid-phase carrier Wang resin, and then the protecting group of the amino group is removed, and the first amino acid is attached to the solid-phase carrier; The carboxyl group of the second amino acid protected by the Fmoc group is activated with a condensing agent, and the activated amino acid reacts with the amino group of the first amino acid attached to the solid support to form a peptide bond. A dipeptide with a protecting group. Repeat the above-mentioned peptide bond formation reaction, so that the peptide chain grows from the C-terminus to the N-terminus until the desired length of the peptide chain is reached, and finally the target polypeptide Glu-Ala-Cys-Ile-Gln-Ala-Cys-Lys (EACIQACK) is obtained by cutt...

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Abstract

The invention belongs to the technical field of food-borne protein deep processing, and particularly relates to a sesame polypeptide. The polypeptide is composed of eight amino acids of which the sequence is as follows: Glu-Ala-Cys-Ile-Gln-Ala-Cys-Lys (EACIQACK). The sesame polypeptide disclosed by the invention can be obtained by separating and purifying enzymolysis sesame protein, and can also be artificially synthesized by adopting a chemical solid-phase synthesis method. By determining the DPPH scavenging capability, the ABTS scavenging capability, the total antioxidant capacity (FRAP) andthe ACE inhibition capability, the invention finds that the polypeptide has strong antioxidant activity and strong antihypertensive activity, can be used as a functional active component in the fields of preparation of medicines, health care products, foods, nutrient supplements, feeds, cosmetics, daily chemical products and the like, and has a wide market prospect.

Description

technical field [0001] The invention belongs to the field of plant protein deep processing, and in particular relates to an active polypeptide EACIQACK derived from sesame 7S protein, a preparation method and its application in antioxidant activity and hypotensive activity. Background technique [0002] At present, functional peptide research is one of the hot spots in food research. Common functional peptides include antioxidant peptides, blood pressure-lowering peptides, antibacterial peptides, and flavor peptides. Previous studies often focus on the study of a single activity of a polypeptide, while ignoring the study of active peptides with multiple activities. Tanzadehpanah et al. structurally modified AFKDEDTEEVPFR to have antihypertensive activity and antioxidant activity. Garcia-Mora et al. enzymatically decomposed lentil protein and found polypeptides with blood pressure lowering activity and antioxidant activity, which indicated that multifunctional active peptide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/04C07K1/18C07K1/16C07K1/34C07K1/36C07K1/14C12P21/06
CPCC07K7/06C12P21/06
Inventor 芦鑫贾聪高锦鸿王瑞丹张丽霞宋国辉孙强黄纪念
Owner HENAN ACAD OF AGRI SCI
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