Application of sodium tetradecyl sulfate in preparation of medicine for treating hemangioma

A technology of sodium tetradecyl sulfate and hemangioma, applied in the direction of drug combination, antineoplastic drugs, active ingredients of anhydride/acid/halide, etc., can solve side effects and other problems, achieve good curative effect and avoid huge trauma

Pending Publication Date: 2020-07-31
SPH NO 1 BIOCHEM & PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment methods for hemangioma mainly include drug therapy, laser therapy, surgical therapy, cryotherapy, etc. There are many methods, each of which has certain curative effect and certain side effects

Method used

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  • Application of sodium tetradecyl sulfate in preparation of medicine for treating hemangioma
  • Application of sodium tetradecyl sulfate in preparation of medicine for treating hemangioma
  • Application of sodium tetradecyl sulfate in preparation of medicine for treating hemangioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Isolation and cultivation of hemangioma cells

[0046] Place the surgically resected hemangioma tissue in serum-free DMEM culture medium, and rinse the blood clot and necrotic tissue of the hemangioma specimen with PBS until the washing solution is clear in color without turbidity and exfoliated tissue; use ophthalmic scissors Use tweezers to remove excess skin and fat, select tumor tissue, and trim it to 1-2cm 3 Add 0.2% type Ⅰ collagenase pre-warmed at 37°C, digest in a water bath at 37°C, shake once every 5 minutes, shake vigorously after 20 minutes of digestion, then add 0.1% trypsin to digest at 37°C for 5 minutes, and finally add 20mL DMEM serum-free medium to stop the digestion, filter with a 100-mesh metal sieve to remove undigested tissue. Trim the digested tissue block to 1mm 3 Size, placed in 2ml fetal bovine serum, let stand for 5min, then suspended in endothelial cell medium (composition: M199 medium, 10% fetal bovine serum, 10U / mL penicillin, 1...

Embodiment 2

[0047] The establishment of embodiment 2 mouse model

[0048] The 3rd generation hemangioma cells collected by trypsinization were digested with 10 8 The cell / mL density was resuspended in complete culture medium, and 200 μL was injected subcutaneously into the bilateral buttocks of 40 nude mice. On the 48th day after injection, 36 of the injected tumor cells survived. There was no big difference in the size of the tumors, the size was about 75mm 3 , were randomly divided into two groups, 18 in each group, marked as group A and group B respectively, group A was injected with 1mL sodium tetradecyl sulfate (2mL: 20mg), twice a day, group B was injected with 1mL normal saline As a control group, twice a day. Observe the growth of the tumor every 7 days, calculate the maximum diameter a and transverse diameter b of the tumor with a vernier caliper, and use the formula V=π / 6×a×b 2 Calculate the volume of the tumor. On the 7th day, 14th day, and 21st day after drug intervention,...

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PUM

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Abstract

The invention discloses application of sodium tetradecyl sulfate in preparation of a medicine for treating hemangioma. According to the invention, a sodium tetradecyl sulfate injection prepared from sodium tetradecyl sulfate is used for experiments, and researches on various in-vitro test systems prove that: sodium tetradecyl sulfate can effectively inhibit endothelium, has obvious effect on damage to endothelial cells and can achieve the purpose of treating the hemangioma. Compared with an existing common treatment method, the medicine prepared from sodium tetradecyl sulfate is minimally invasive, traceless, economical, repeatable and good in treatment effect when used for treating the hemangioma, part of patients with large lesions can be treated multiple times in stages, and huge trauma, residues and scars of open surgery are avoided.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to the application of sodium tetradecyl sulfate in the preparation of medicines for treating hemangiomas. Background technique [0002] Sodium tetradecyl sulfate has made a breakthrough in the treatment of sclerotherapy injections due to its high curative effect and small side effects. Sodium tetradecyl sulfate structural formula is shown in following formula I: [0003] [0004] At present, sodium tetradecyl sulfate is widely used in the clinical treatment of varicose veins of the lower extremities, and the curative effect is remarkable. Patent CN104840419B has prepared a sodium tetradecyl sulfate injection with good stability. [0005] Hemangioma is the most common benign vascular hyperplasia or vascular malformation in infants and young children. It is prone to occur in facial skin, subcutaneous tissue, and oral mucosa, such as tongue, lip, and floor of the mouth. Facial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/185A61P35/00
CPCA61K31/185A61P35/00
Inventor 顾春燕陈辰黄臻辉
Owner SPH NO 1 BIOCHEM & PHARMA CO LTD
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