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DNA rapid identification method for ivories and products thereof

An identification method and product technology, applied in the biological field, to achieve the effects of improving detection efficiency, avoiding pollution, and saving electrophoresis time

Active Publication Date: 2020-07-28
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

precipitationfor DNA extraction

Method used

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  • DNA rapid identification method for ivories and products thereof
  • DNA rapid identification method for ivories and products thereof
  • DNA rapid identification method for ivories and products thereof

Examples

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Embodiment Construction

[0027] In order to make the content of the present invention easier to understand, the technical solutions of the present invention will be further described below in conjunction with specific embodiments, but the following examples are only examples of the present invention, and do not represent limitations of the present invention The scope of protection of rights, the scope of protection of rights of the present invention shall be determined by the claims.

[0028] Real-time example 1

[0029] 1. Test material

[0030] Ivory and its products are collected by this laboratory.

[0031] 2. Main reagents

[0032] Lysis solution [5mol / L guanidine isothiocyanate, 0.05 mol / L Tris-HCl (pH6.4), 0.02 mol / L EDTA (pH8.0), 1.3% Triton X-100 (v / v)] self-prepared ; SYBR Premix Ex Taq (2×) is a product of Bao Biological Engineering (Dalian) Co., Ltd. (Dalian TaKaRa Company).

[0033] 3. Main instruments and equipment

[0034] Mixing mill, vortex instrument, water bath, ultrasonic inst...

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Abstract

The invention provides a DNA rapid identification method for ivories and products thereof. By comparing different decalcification treatment, an ultrasonic treatment method (ultrasonic treatment for 2hat 37 DEG C) with a good decalcification effect and short consumed time is screened out. Extracted DNA is amplified with an SYBR Green real-time fluorescent polymerase chain reaction (PCR) method instead of a common PCR method, and without a subsequent PCR product electrophoresis process, sequence determination is directly performed on a real-time fluorescent PCR product with an amplification curve and a melting curve and of which the melting temperature is 82.00 DEG C+ / -0.5 DEG C, so that experimental procedures and laboratory pollution are reduced; and ultrasonic decalcification and the SYBR Green real-time fluorescent PCR method are combined, and thus, the experimental time is greatly shortened, and the species of the ivories and the products thereof can be quickly identified.

Description

technical field [0001] The invention relates to a DNA rapid identification method for ivory and its products, belonging to the field of biotechnology. Background technique [0002] Ivory smuggling is becoming more and more rampant. In order to effectively combat ivory smuggling and protect wild endangered animals, ivory species identification technology is urgently needed. The types of ivory mainly include: mammoth ( Mammuthus primigenius ) tusk, Asian elephant ( Elephas maximus Linnaeus) Tusk, African Elephant ( Loxodonta africana ) tusks, among which mammoth tusks are fossils, and Asian elephants and African elephants are endangered species. After the three types of ivory are processed into various tourism products or works of art, it is difficult to distinguish the species from the morphology, but molecular biological methods can be used to accurately identify the species to which the ivory belongs. DNA extraction is a key link in molecular biology identification ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/10C12Q1/6806C12Q1/686C12N15/11
CPCC12Q1/6888C12N15/1003C12Q1/686C12Q1/6806C12Q2561/113C12Q2563/107
Inventor 邵碧英陈文炳彭娟缪婷玉
Owner INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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