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Application of microbial metabolite in relieving glucose and lipid metabolism disorder

A technology of use and medicine, which is applied in the field of improving glucose tolerance, improving hepatic steatosis, and alleviating weight gain. It can solve the problems of inconvenient promotion, anaerobic and harsh culture regulation, etc., so as to improve glucose tolerance, relieve excessive body deposition, and improve liver The effect of steatosis

Active Publication Date: 2020-07-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Akkermansia muciniphila (AM) strain is a new type of second-generation probiotic with weight-loss effect discovered in recent years, but due to its anaerobic and harsh culture regulation, it is inconvenient to be widely promoted in production

Method used

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  • Application of microbial metabolite in relieving glucose and lipid metabolism disorder
  • Application of microbial metabolite in relieving glucose and lipid metabolism disorder
  • Application of microbial metabolite in relieving glucose and lipid metabolism disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] experimental animals

[0053] In the experiment, 90 male C57BL / 6 mice aged 3 weeks with SPF grade (purchased from Beijing Huafukang Experimental Animal Technology Co., Ltd.) were selected and raised in a mouse room at 22±1°C, and the light time was 12 hours a day (8:00 to 19:00). 20 o'clock), ad libitum intake of food and water, after a week of adaptation, the formal test began.

[0054] Reagents and Materials

[0055] pET-26b(+) expression vector: purchased from Addgene, USA, kanamycin resistant, with a T7 promoter that can be activated by isopropylthiogalactoside (IPTG)

[0056] Ni-NTA agarose purification resin: purchased from GE, USA

[0057] Brain-heart infiltration medium powder, agar, tryptone, yeast extract: purchased from BD Company in the United States

[0058] XhoI and NdeI restriction enzymes and DNA ligation kit: purchased from Dalian Takara Company

[0059] Competent Escherichia coli TOP10 (ZC104) and TOP10 BL21 (DE3): purchased from Beijing Zhuang...

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Abstract

The invention relates to application of a microbial metabolite in relieving glucose and lipid metabolism disorder, and particularly relates to application of AMP protein in preventing and / or treatingobesity, fatty liver, diabetes and hyperlipidemia. By means of the application, the body weight gain and the blood fat content induced by high fat can be effectively reduced, the glucose tolerance isimproved, deposition of subcutaneous fat and visceral fat is reduced, liver damage and liver fatty degeneration are relieved, and the AMP protein has great significance in relieving obesity and fattyliver and is wide in application prospect.

Description

technical field [0001] The present invention relates to the field of biology. Specifically, the present invention relates to the application of a microbial metabolite, AMP protein, in alleviating fat deposition, improving glucose tolerance and improving hepatic steatosis. More specifically, the present invention relates to the use of microbial metabolites, namely AMP protein, in alleviating fat deposition, improving glucose tolerance and improving hepatic steatosis, as well as in alleviating weight gain, improving glucose tolerance and improving hepatic steatosis. Background technique [0002] The prevalence of obesity has increased globally over the past few decades. Globally, more than 600 million adults and 100 million children are reported to be obese. Overweight and obese individuals are prone to cause a series of diseases, such as type 2 diabetes, cardiovascular disease, cancer and other metabolic diseases with abnormal metabolism, so obesity has become an urgent pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P3/04A61P1/16A61P3/10A61P3/06
CPCA61K38/164A61P1/16A61P3/04A61P3/06A61P3/10
Inventor 武振龙陈菁青杨鹰
Owner CHINA AGRI UNIV
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