Novel beta coronavirus variation detection method, probe and kit

Abstract

The invention relates to a novel beta coronavirus (SARS-CoV-2) variation detection kit. The kit is characterized by comprising the following parts: an RNA probe group, primers, a blocking buffer, an RNA enzyme blocking solution, a hybridization buffer, a binding buffer, an SSC rinsing solution with 1 time concentration, an SSC rinsing solution with 0.1 time concentration, a PCR reaction solution and a TE buffer, wherein the RNA probe group comprises 311 RNA probes, and the sequences of the RNA probes are shown as SEQ ID NO.1 to SEQ ID NO.311. The novel beta coronavirus (SARS-CoV-2) variation detection kit provided by the invention can enrich beta coronavirus nucleic acid sequences in a library, improves the detection sensitivity, detects beta coronaviruses of all people and animals at thesame time, and is used for beta coronavirus detection and hereditary variation detection.

Description

technical field [0001] The invention relates to the field of biological gene detection, in particular to a novel betacoronavirus (SARS-CoV-2) mutation detection method, probe and kit based on liquid-phase probe hybridization capture. Background technique [0002] SARS-CoV-2 and SARS coronavirus and "Middle East Respiratory Syndrome" MERS coronavirus belong to the genus βcoronavirus, and the genome is linear positive-strand RNA. As an RNA virus, the new coronavirus has a high mutation frequency, and it will mutate more during the transmission between wild animals-intermediate hosts-humans. The 2019-nCoV nucleic acid detection kit based on the real-time quantitative PCR (RT-qPCR) method only conducts qualitative detection for the specific conserved sequence region of the 2019-nCoV or the antigen of a certain strain initially released. This method has low tolerance for genetic variation. If the virus gene mutation occurs in the specific binding region of the primer and probe,...

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Problems solved by technology

However, the amount of sequencing data required by this method is huge, and the cost of sequencing is high; and this method is aimed at samples from patients in the occult period or samples with low virus abundance, and there is no upper limit on the amount of data required, so it is difficult to splice the obtained sequencing results into a full-length Sequence, difficult to detect viral gene sequence variation

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