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Method for extracting tea polysaccharide from tea residues

A tea polysaccharide and tea dregs technology, which is applied in the direction of anti-toxic agents and drug combinations, can solve the problems of low extraction rate, low extraction efficiency, and short extraction time, and achieve improved wall-breaking efficiency, extraction efficiency, and extraction speed , the effect of improving the yield and antioxidant activity

Active Publication Date: 2020-07-24
湘丰茶业集团有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tea polysaccharide extraction methods mainly include hot water extraction, ultrafiltration extraction, enzymatic extraction, ultrasonic extraction, microwave extraction, etc. Hot water extraction is the most common method, which is simple to operate, but the extraction efficiency is not high. Extraction is easy to affect the biological activity of tea polysaccharides. Enzymatic extraction has mild conditions, and the release of polysaccharides is accelerated by adding saccharification enzymes or protein enzymes. Ultrasonic extraction uses the cavitation effect of ultrasound to improve the activity of tea polysaccharides. Extraction efficiency and yield, the microwave method uses microwave energy, the extraction time is short and the efficiency is high, but it is only suitable for heat-stable extracts
Although there are a variety of extraction methods, tea residues are used as raw materials for extraction. The extraction methods currently reported still have problems such as low extraction rate and low purity, and there are few reports on comprehensive extraction methods with high yields.

Method used

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  • Method for extracting tea polysaccharide from tea residues

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Comparison scheme
Effect test

Embodiment 1

[0048] Crush the tea residue, pass through a 100-mesh sieve, degrease with absolute ethanol, wash the tea residue powder at 75°C for 30 minutes, centrifuge at 2000r / min for 10 minutes, remove the supernatant, and dry it at 40°C and 50MPa Dry under vacuum for 30 minutes to obtain fine powder of tea residue; add water to the tea residue powder according to the ratio of material to liquid of 1:35, control the water temperature to 70°C, set the microwave power to 500 W, and break the wall by microwave for 80 minutes; according to the ratio of 1:1 Add cellulase and pectinase with a mass ratio of 0.1% of the solution mass, adjust the solution temperature to 45°C, set the pH value to 6.5, set the ultrasonic frequency to 35KHz, low-frequency enzymatic hydrolysis for 50min, adjust the solution temperature to 65°C, and set the ultrasonic frequency to 60KHz, high-frequency enzymatic hydrolysis for 40 minutes; inactivate the solution at 110°C for 15 minutes, put it in a centrifuge and cent...

Embodiment 2

[0050] Crush the tea residue, pass through a 150-mesh sieve, degrease with absolute ethanol, wash the tea residue powder at 75°C for 40 minutes, centrifuge at a speed of 2000r / min for 10 minutes, remove the supernatant, and dry it at 40°C and 50MPa Dry under vacuum for 30 minutes to obtain tea residue fine powder; add water to the tea residue powder according to the ratio of material to liquid at 1:40, control the water temperature at 75°C, set the microwave power to 550 W, and microwave the wall breaking for 70 minutes; according to the ratio of 1:1.5 Add cellulase and pectinase with a mass ratio of 0.15% of the solution mass, adjust the solution temperature to 50°C, and set the pH value to 6.6. 65KHz, high-frequency enzymatic hydrolysis for 35 minutes; inactivate the solution at 115°C for 12 minutes, put it in a centrifuge and centrifuge at a speed of 3300r / min for 11 minutes, remove the residue after vacuum filtration, and place the filtrate in a rotary evaporator. Concentr...

Embodiment 3

[0052] Crush the tea residue, pass through a 200-mesh sieve, degrease with absolute ethanol, wash the tea residue powder at 75°C for 50 minutes, centrifuge at 2000r / min for 10 minutes, remove the supernatant, and dry it at 40°C and 50MPa Dry under vacuum for 30 minutes to obtain tea residue fine powder; add water to the tea residue powder according to the material-liquid ratio of 1:45, control the water temperature at 80°C, set the microwave power to 600 W, and microwave the wall breaking for 60 minutes; according to the ratio of 1:2 Add cellulase and pectinase with a mass ratio of 0.2% of the solution mass, adjust the solution temperature to 55°C, set the pH value to 6.8, set the ultrasonic frequency to 45KHz, low-frequency enzymatic hydrolysis for 40min, adjust the solution temperature to 75°C, and set the ultrasonic frequency to 70KHz, high-frequency enzymatic hydrolysis for 30min; inactivate the solution at 120°C for 10min, put it in a centrifuge and centrifuge at a speed o...

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Abstract

The invention discloses a method for extracting tea polysaccharide from tea residues. The method comprises the following steps: 1, tea residue pretreatment; 2, microwave wall breaking; 3, variable-frequency ultrasonic composite enzymolysis; 4, centrifugal concentration; 5, alcohol precipitation; and 6, washing and drying. According to the method, the advantages of various extraction methods are combined, the technical characteristics of microwave, ultrasonic wave and enzyme treatment are effectively combined, the wall breaking efficiency is effectively improved, the polysaccharide structure isnot damaged, and the yield and the antioxidant activity of the polysaccharide are remarkably improved.

Description

technical field [0001] The invention belongs to the field of tea deep processing, and in particular relates to a method for extracting tea polysaccharide from tea dregs. Background technique [0002] Large-scale tea deep-processing plants such as tea beverages, tea polyphenols, and catechins produce a large amount of tea dregs during the production process. The normal production process only uses the water-soluble components in the tea leaves, and there are still some tea residues in the tea dregs. Effective substances such as polyphenols, amino acids, crude protein, crude fiber, and tea residue have high utilization value; tea polysaccharide is a compound polysaccharide, which is composed of sugar, pectin and protein. The specific composition is: total sugar content 30.92% , protein 17.87%, moisture 8.89%, ash 16.48%, pectin 12.92%, other 12.92%. Tea polysaccharides have physiological functions such as lowering blood sugar, lowering blood pressure, resisting hypoxia, incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06
CPCA61P39/06C08B37/0003
Inventor 杨庆新刘仲华黄建安施玲胡云铃
Owner 湘丰茶业集团有限公司
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