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A kind of comprehensive treatment method of rhamnolipid fermentation liquid

A rhamnolipid and comprehensive treatment technology, applied in the field of fermentation engineering, can solve the problems of high production cost of rhamnolipid, high cost of three wastes treatment, complicated separation process, etc., achieve no complex conversion equipment, increase product added value, Simple effect of conversion process

Active Publication Date: 2022-04-19
WANHUA CHEM (SICHUAN) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] What the present invention is to solve is that in the prior art, the separation difficulty of rhamnolipid fermentation liquid is large, the separation process is complicated, the cost of three wastes is high, and the processing difficulty is large, thereby leading to the problem of high production cost of rhamnolipids. The purpose of the present invention is to A comprehensive treatment method for rhamnolipid fermentation liquid is provided, through which not only high-purity rhamnolipids can be produced, but also the three fermentation wastes generated in the production process can be effectively utilized to realize regeneration and utilization and turn waste into treasure, thereby Reduce the production cost of rhamnolipid and reduce the discharge of three wastes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The comprehensive processing method of the present embodiment rhamnolipid fermentation liquid comprises the following steps:

[0045] (1) Add 230g of nylon acid to adjust the pH value to 2.9 in 5L rhamnolipid fermented liquid (solid content is about 8%, after measuring rhamnolipid content is 105g / L, isoelectric point is 2.9, makes solid precipitation;

[0046] (2) Use a positive pressure filter with a 5 μm pore diameter filter membrane to filter to obtain 1.8 kg of filter cake a and 3.43 kg of filtrate a, add 3.6 kg of absolute ethanol to the filter cake a, and stir at 35°C for 60 minutes to make rhamnose The lipid is fully dissolved to obtain a rhamnolipid suspension;

[0047] (3) Filter the rhamnolipid suspension with a positive pressure filter of 2 μm pore size filter membrane to obtain filter cake b and 4.7 kg of filtrate b, and decompress filtrate b at a vacuum degree of 50 Pa and a temperature of 55° C. Concentration (rotary evaporation to dryness) until no liqu...

Embodiment 2

[0051] The comprehensive processing method of the present embodiment rhamnolipid fermentation liquid comprises the following steps:

[0052] (1) Add 320g of nylon acid to adjust the pH value to 3.7 in 10L rhamnolipid fermentation broth (solid content is about 10%, the measured rhamnolipid content is 75g / L, and the isoelectric point is 3.75), so that the solid precipitation;

[0053] (2) Use a positive pressure filter with a 5 μm pore diameter filter membrane to filter to obtain 2.5 kg of filter cake a and 10.5 kg of filtrate a, add 25 kg of absolute ethanol to the filter cake a, and stir for 30 minutes at 20° C. to make the rhamnolipid Fully dissolved to obtain a rhamnolipid suspension;

[0054] (3) Rhamnolipid suspension is filtered with a positive pressure filter of 2 μm aperture filter membrane to obtain filter cake b and 26 kg of filtrate b, and filtrate b is concentrated under reduced pressure at a vacuum of 200 Pa and a temperature of 65° C. ( Rotary evaporated to dryn...

Embodiment 3

[0058] The comprehensive processing method of the present embodiment rhamnolipid fermentation liquid comprises the following steps:

[0059] (1) in 8L rhamnolipid fermented liquid (solid content is about 10%, after measuring rhamnolipid content is 125g / L, isoelectric point is 2.75), adds 511g nylon acid and adjusts pH value to 2.7, makes solid precipitation;

[0060] (2) Use a positive pressure filter with a 5 μm pore diameter filter membrane to filter to obtain 2.8 kg of filter cake a and 5.7 kg of filtrate a. Add 14 kg of absolute ethanol to the filter cake a and stir for 40 minutes at 30° C. to make the rhamnolipid Fully dissolved to obtain a rhamnolipid suspension;

[0061] (3) Rhamnolipid suspension is filtered with a positive pressure filter of 2 μm aperture filter membrane to obtain filter cake b and 16kg filtrate b, and filtrate b is concentrated under reduced pressure at a vacuum of 100 Pa and a temperature of 60°C ( Rotary evaporated to dryness), until no liquid dr...

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Abstract

The invention relates to a comprehensive treatment method of rhamnolipid fermentation liquid. The method comprises the following steps: adding nylon acid to the rhamnolipid fermentation broth, adjusting the pH value to be lower than the isoelectric point, and causing solid precipitation; filtering to obtain filter cake a and filtrate a, and adding ethanol to filter cake a to obtain Rhamnolipid suspension; filter to obtain filter cake b and filtrate b, filtrate b is concentrated under reduced pressure to obtain purified rhamnolipid; filter cake b is dried and pulverized to obtain organic nitrogen fertilizer; filtrate a is added to glycerin, liquid Layering, the plasticizer polynylonate hydroxypropylene glycol ester is obtained from the upper organic phase, and the rhamnolipid fermentation broth is obtained from the lower aqueous phase. The method can obtain the rhamnolipid with a purity greater than 95% in a yield of greater than 92% through three-step separation, and the separation route is simple; at the same time, the waste residue and waste water are converted into nylon acid hydroxypropylene glycol ester plasticizer, organic nitrogen fertilizer and rhamnolipid The glycolipid fermentation culture fluid reduces the production cost and the discharge of three wastes, which is beneficial to its wide application.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a comprehensive treatment method for rhamnolipid fermentation liquid. Background technique [0002] Rhamnolipid is mainly metabolized by Pseudomonas aeruginosa in a certain environment. It has good surface activity and interfacial activity and can be widely used in petrochemical, environmental, pharmaceutical, food and agricultural fields; it is non-toxic , The advantage of being biodegradable is a biosurfactant with the longest research time, the best effect and the most mature application technology. [0003] In addition to rhamnolipids, the rhamnolipid fermentation broth also contains impurities such as fermentation cells, proteins, and inorganic salts. Generally, the crude rhamnolipid products are obtained through centrifugation and acid precipitation, and then undergo extraction, membrane separation, and column extraction. High-purity rhamnolipids can onl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/06
CPCC05C11/00C07H15/04C07H1/06C08G69/26
Inventor 石森王竞辉李晶黄真真王勤隆周芸孔令晓黄长荣黎源
Owner WANHUA CHEM (SICHUAN) CO LTD
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