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Magnetic beads for detection of COVID-19 and preparation method of magnetic beads

A technology of pneumonia and magnetic beads, which is applied in the field of nanomaterials and bacteria inspection, can solve the problems of low content of new coronavirus, unsuitable clinical detection, and high technical difficulty, so as to increase biocompatibility and chemical stability, and improve detection efficiency and positive rate, low cost effect

Inactive Publication Date: 2020-07-14
李长桂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technology is difficult, the operation is complicated, the time required is long, and the requirements for equipment are quite high, so it is not suitable for clinical testing.
In addition, the content of 2019-nCoV in samples provided by patients is often very low, especially only traces of pneumococcus in washing liquid, which poses a great challenge for diagnosis

Method used

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  • Magnetic beads for detection of COVID-19 and preparation method of magnetic beads
  • Magnetic beads for detection of COVID-19 and preparation method of magnetic beads

Examples

Experimental program
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Effect test

Embodiment 1

[0030] This embodiment provides a magnetic bead for the detection of new coronary pneumonia, including Fe 3 o 4 / SiO 2 / PMO nano magnetic beads and coated in Fe 3 o 4 / SiO 2 / Angiotensin-converting enzyme 2 (ACE2) on the surface of PMO nanomagnetic beads.

[0031] The Fe 3 o 4 / SiO 2 The preparation method of / PMO nano-magnetic beads, comprises the steps:

[0032] S1, the FeCl 2 4H 2 O, FeCl 3 ·6H 2 O was dissolved in diethylene glycol at a molar ratio of 1:2 to make Fe 2+ The concentration is 0.05mol / L, add dextran, heat and stir at 200°C for 20 minutes, then add NaOH, continue heating and stirring for 2 hours, cool to room temperature after the reaction, centrifuge and wash to obtain Fe 3 o 4 Nano magnetic beads; the dextran concentration is 3%; the NaOH concentration is 10%;

[0033] S2, with the Fe that step S1 makes 3 o 4 Nano-magnetic beads are dispersed in alcohol-water mixture, then ammonia water and tetraethoxysilane are added, and the reaction is stir...

Embodiment 2-5

[0040] Embodiments 2-5 provide a magnetic bead for detection of new coronary pneumonia, compared with Example 1, the difference is that the Fe in the preparation method of the magnetic bead for detection of new coronary pneumonia is changed. 3 o 4 / SiO 2 / PMO nano magnetic beads and angiotensin-converting enzyme 2 (ACE2) coupling solution, all the other are the same as in Example 1, and will not repeat them here.

[0041] Example Dosage ratio (mg: mL) Adsorption efficiency (%) 1 1:10 99.9 2 1:0 87 3 1:5 98 4 1:15 99 5 1:20 91

[0042] As can be seen from the results in the above table, changing the Fe in the preparation method of the magnetic beads for the detection of new coronary pneumonia 3 o 4 / SiO 2 The ratio of the amount of / PMO nano-magnetic beads to angiotensin-converting enzyme 2 (ACE2) coupling solution will have a significant impact on the adsorption performance of the prepared nano-magnetic beads, and with the inc...

Embodiment 6-9

[0044] Embodiment 6-9 provides a kind of magnetic bead for new coronary pneumonia detection, compared with embodiment 1, the difference is that the Fc 3 o 4 / SiO 2 The amount of dextran used in step S1 of the preparation method of PMO nano magnetic beads is the same as that in Example 1, and will not be repeated here.

[0045] Example Dextran dosage (%) Adsorption efficiency (%) 6 0 85 7 2 96 8 5 99 9 6 99

[0046] As can be seen from the results in the table above, changing the Fe 3 o 4 / SiO 2 The amount of dextran in the step S1 of the PMO nano-magnetic bead preparation method will have a significant impact on the adsorption performance of the prepared nano-magnetic bead. At the same time, comparing the results of Example 1 and Example 6, it can be known that the present invention uses dextran Preparation of Fe as a modifier 3 o 4 / SiO 2 / PMO nano magnetic beads can synergistically increase Fe 3 o 4 / SiO 2 The specific adsor...

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Abstract

The invention discloses magnetic beads for detection of COVID-19 and a preparation method of the magnetic beads. The preparation method comprises the steps of firstly, synthesizing dextran-modified Fe3O4 nano magnetic beads by adopting a high-temperature polyol method, and then preparing Fe3O4 / SiO2 / PMO composite nano magnetic beads with a yolk-shell structure by adopting a simple and controllablegrowth-induced corrosion method; and coupling angiotensin converting enzyme 2 (ACE2) to the dextran-modified Fe3O4 / SiO2 / PMO nano magnetic beads. The ACE2 is specifically bound with novel coronavirus in an adsorption process by utilizing the specificity of the ACE2 to novel coronavirus, thereby quickly separating and enriching novel coronavirus from a complex sample with extremely low novel coronavirus content to improve the detection efficiency and positive rate of the COVID-19. The magnetic beads have the advantages of high accuracy, high sensitivity, simple and convenient operation and strong specificity, thereby providing an effective way for early screening of the COVID-19.

Description

technical field [0001] The invention relates to the technical field of nanomaterials and bacterial detection, in particular to a magnetic bead for detection of new coronary pneumonia and a preparation method thereof. Background technique [0002] Since the outbreak of novel coronavirus disease 2019 (COVID-19), the epidemic has spread rapidly. In view of the great harm of COVID-19 to public health, governments at all levels, health and epidemic prevention, medical treatment, and scientific research departments attach great importance to pathogen identification, traceability, case screening, crowd isolation, disease diagnosis, vaccines and new drugs, and treatment plans, etc. A lot of research has been done. Since the disease is a new infectious disease, there is a lack of in-depth understanding of its pathogenesis, and there is still a lack of specific and effective drugs and vaccines. The treatment measures are mostly symptomatic and supportive care. [0003] The basic pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10G01N33/569C08G77/26C08K7/18C08K3/36
CPCC08G77/26C08K3/36C08K7/18C12N9/485C12N11/10C12N11/14C12Y304/17023G01N33/56983G01N2333/165
Inventor 李长桂
Owner 李长桂
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