A kind of anti-glioma polypeptide molecule and its application

A technology for glioma and glioma cells, which is applied in the direction of polypeptides containing localization/targeting motifs, anti-tumor drugs, peptide/protein components, etc., and can solve problems such as inability to accurately target gliomas

Active Publication Date: 2020-12-22
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Another object of the present invention is to provide an anti-glioma polypeptide molecule in the preparation of anti-glioma drugs, since the polypeptide molecule can accurately target brain glioma, it can solve the current problem of inability to accurately target glioma. tumor problem, realized the diagnosis of glioma or guided clinical medication

Method used

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  • A kind of anti-glioma polypeptide molecule and its application
  • A kind of anti-glioma polypeptide molecule and its application
  • A kind of anti-glioma polypeptide molecule and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The purity of TAT-PICK1 was analyzed by analytical high performance liquid chromatography (flow rate: 1ml / min). Specifically:

[0028] The model of the analytical high-performance liquid chromatograph: Agilent 1200, the model of the chromatographic column used: AngilentEclipse XDB-C18 Analytical, 5um, 4.6X150006Dm.

[0029] Chromatographic operating conditions: linear gradient elution, the eluent is composed of mobile phase A liquid and B liquid. Mobile phase A liquid: 0.1% by volume concentration of trifluoroacetic acid aqueous solution (water as solvent), mobile phase B liquid: 0.1% by volume concentration of trifluoroacetic acid in acetonitrile solution (acetonitrile as solvent). The elution time was 12 minutes in total, the elution flow rate was 1 mL / min, and the ultraviolet detection wavelength was 220 nm.

[0030] The detection result of analytical high performance liquid chromatography showed that the purity of the obtained TAT-PICK1 was 97.2%.

Embodiment 2

[0032] The chemical structure of TAT-PICK1 was characterized by MALDI-TOF mass spectrometry, and the mass spectrometry characterization results of TAT-PICK1 were as follows figure 2 shown. Molecular weight (Mw): 2825.30, the other three peaks can also prove that the molecular weight of TAT-PICK1 is consistent.

Embodiment 3

[0034] TAT-PICK1 polypeptide molecule inhibits the migration of glioma cell C6

[0035] Transwell plate (Corning Company, product number #3422) was used to detect the effect of TAT-PICK1 polypeptide on the C6 migration ability of glioma cells. Cell migration experiments were performed according to the instructions of the Transwell plate. The main operation steps are as follows:

[0036] 1) Dilute the digested and counted glioma cell C6 and TAT-PICK1 polypeptide to 200,000 / mL with serum-free culture medium DMEM (final concentration is 30uM), take 200uL and add it to the upper chamber of the Transwell, and add 600uL to the lower chamber of the Transwell to completely The cell culture solution was used as the experimental group; the serum-free culture solution containing the TAT leader peptide was used as the negative control group.

[0037] 2) in CO 2 37°C, 5% CO in an incubator 2 Continue to culture for 24 hours under the same conditions, scrape off the cells in the upper T...

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Abstract

The invention discloses an anti-glioma polypeptide molecule which consists of a leader peptide TAT and PICK1. The invention also discloses an application of the anti-glioma polypeptide molecule in preparing anti-glioma medicaments and an application of the anti-glioma polypeptide molecule in a nude mouse subcutaneous glioma model. The polypeptide molecule disclosed by the invention penetrates through cell membranes to be combined with the intracellular TLR4 so as to cause endocytosis, so that the inflammatory pathway of the polypeptide molecule is blocked, and generation and development of gliomas are realized. Therefore, the invention provides guidance for the fields related to drug guidance and treatment methods, and provides theoretical basis for target diagnosis of gliomas and preparation of treatment drugs.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a polypeptide molecule against glioma and its application. Background technique [0002] Glioma is the most common primary malignant tumor of the nervous system, accounting for more than 45% of central nervous system tumors and seriously threatening human health. The World Health Organization divides it into 4 grades, grades I and II are low-grade gliomas, grades III and IV are high-grade malignant gliomas (neuroblastoma), and the incidence rate of malignant gliomas is 3 / 10000. However, it has the characteristics of strong infiltration ability, easy invasion, high recurrence rate and mortality rate, and the average survival period of patients is only 12-15 months. At present, malignant glioma mainly adopts surgical resection, radiotherapy, chemotherapy and other treatment methods, but the prognosis of patients is still poor and the survival period is short. With the development of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61K38/17A61K47/64A61P35/00
CPCA61K38/00A61P35/00C07K14/47C07K2319/10
Inventor 王震张威李凯强王倩妮陈秉宇郝珂程晶晶吴玲玲
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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